Abstract: The present teachings relate to methods, kits and devices for performing automated sequential nucleic acid isolation and conversion/purification in a single closed system. In various embodiments, the present teaching enable a user to (i) load a device with test samples, reagents and consumables; (ii) select or program the device for the desired nucleic acid isolation and subsequent chemical treatment and/or conversion reaction(s) without further user intervention; and recovering the isolated and treated and/or converted nucleic acid at the conclusion of the program once the device is activated.

Abstract: The present invention provides a composition, a kit and the use thereof, as well as the method for detecting the cell proliferative abnormality in individuals or grading the disease degree in the individuals. The composition comprises nucleic acids for detecting the methylation level within at least one target region of a gene and the fragment thereof.

Abstract: The invention relates to a method for detecting cancer or precancerous condition in a subject. It uses rhPCR based target enrichment of nucleic acid for better sensitivity and specificity for sequencing and analysis. The method provides use of cfDNA form plasma, serum or other bodily fluids of the subject.

Abstract: The present teaching generally discloses a method of measuring the concentration of an analyte in a solution. The method includes selecting a binding compound; selecting an analog to an analyte and conjugating the analog to a polynucleotide substrate to construct a reagent for measuring the amount of the analyte in an assay solution using a desired polynucleotide reaction that comprises a polymerization reaction, a cleavage reaction, or a recombination reaction. The binding of the reagent to the binding compound in the assay solution competes with the binding of the analyte to the binding compound in the assay solution. The method also includes selecting a desired enzyme for catalyzing the desired polynucleotide reaction. Reagents and kits are also provided.

Abstract: This invention presents the high throughput (HT) analysis technology for molecular fractions employing micro-array format. Molecules are fractionated according to the properties of molecules and are assigned primary designated orders while their tissue origins are assigned secondary designated orders such that each fractionated molecules become addressable and traceable. Fractionated molecules are recovered, arrayed, and analyzed according to the same primary designated orders and secondary designated order in a high throughput manner. The above properties of molecules can be measured and predicted.

Abstract: This invention presents a set of methods to extract DNA, RNA and protein simultaneously from biomaterials by reagents with high pH. DNA and RNA can be extracted from a upper aqueous phase simultaneously either together as a DNA and RNA mixture or separated DNA and RNA. Protein can be extracted from lower organic phase. The DNA and RNA mixture can be used either as DNA or RNA directly depending on applications without further separation, or as resource for the separated DNA and RNA that can be selected from the DNA and RNA mixture by selective precipitation and/or by selective enzyme digestions. This invention provides the choice of extraction either of DNA and RNA mixture or of separated DNA and RNA simultaneously, as well as extraction of protein from the same piece of biomaterials in high quality, which is very critical for biomaterials with limited resource, such as clinical specimens.

Abstract: The invention provides methods of separating and isolating protein from at least three subcellular compartments of a single biological sample in a single container. Methods are also provided for determining the subcellular compartmentalization of proteins of interest in a biological sample. Furthermore, methods are provided for fingerprinting a biological sample, as well as identifying a sample type, a cancerous tissue or a developmental stage of a tissue sample. Kits for separating and isolating subcellularly compartmentalized proteins in a biological sample are also provided.

Abstract: This invention presents the high throughput (HT) analysis technology for molecular fractions employing micro-array format. Molecules are fractionated according to the properties of molecules and are assigned primary designated orders while their tissue origins are assigned secondary designated orders such that each fractionated molecules become addressable and traceable. Fractionated molecules are recovered, arrayed, and analyzed according to the same primary designated orders and secondary designated order in a high throughput manner. The above properties of molecules can be measured and predicted. This invention provides advantages such as higher sensitivity, lower consume of materials for molecules, high throughput platform and capability to create database directly when compared to existing analysis methods for molecular fractions.

Abstract: This invention concerns novel methods and compositions useful for directly and rapidly analyzing the biochemical components of microorganisms. The compositions and methods of the present invention obviate the need for extracting and purifying the biochemicals prior to analysis.