Abstract: Immunologic assay for biological and pharmaceutical substances sets forth a universal method for the qualitative and quantitative determination of biologic and pharmaceutical substances, thus eliminating the need for multiple samples at multiple laboratories and minimizing the time factor required for such determinations. The assay is based on the recognition and binding of an antibody and an antigen or hapten to form a complex, and the changes which occur in the antibody conformation and chemical properties when such a complex is formed.A solid phase matrix, such as but not limited to a microtiter plate, is prepared having qualitative and/or quantitative spectrum of antibodies, or spectrum of antigens or haptens, bound in each position, which in the case of microtiter plates would be a sample well. The sample is then distributed to each of the positions or wells accordingly.