Abstract: The present invention relates to a apparatus for quantitative continuous real-time monitoring to monitor a continuous reaction of biochemical reagent and the reaction, such as DNA. More particularly, the present invention is directed to a miniaturized apparatus for real-time monitoring of biochemical reaction, which comprises capillary tubes (100) wherein biochemical reaction mixture flow; a thermal conduction block (120) which is coiled with capillary tube several rounds in order and composed of several blocks for temperature control of which temperatures are different from each other for heating or cooling the biochemical reaction mixture which flow in capillary tube, and a temperature controller which controls the temperature of above temperature control block; a radiation part (130) to radiate the reaction mixture flowing through the capillary tube and a light receiving section (140) which receives and measures the intensity of the fluorescence generated from the capillary tubes.

Abstract: Disclosed is a conjugate for gene transfer, which is capable of being used for treatment of incurable diseases, comprising an oligonucleotide intended to be transferred into target cells and a hydrophilic polymer, wherein an end of the oligonucleotide is covalently conjugated to the hydrophilic polymer. Also, the present invention discloses polyelectrolyte complex micelles formed from such a conjugate and a cationic polymer or cationic peptide. Such polyelectrolyte complex micelles can effectively transfer oligonucleotides as therapeutic agents into target cells, making it possible to obtain desired activities of the delivered oligonucleotides in target cells even when the micelles are clinically applied at a relatively low concentration. Therefore, the conjugate and the polyelectrolyte complex micelle are very useful in basic life science research and the medical field.

Abstract: The present invention relates to a lactic acid bacterium isolated from human mother's milk, more precisely a Lactobacillus gasseri BNR17 strain that is isolated from Korean mother's milk and has excellent probiotic activity including acid resistance, bile acid resistance and antimicrobial activity and weight gaining inhibitory effect as well. Again, the Lactobacillus gasseri BNR17 of the present invention has excellent acid resistance, bile acid resistance, enteric absorption activity and antimicrobial activity against pathogenic microorganisms, in addition to the weight gaining inhibitory effect by synthesizing indigestible polysaccharides from monosaccharides included in food taken and releasing the synthesized polysaccharides out of the body.

Abstract: The present invention relates to a gold-plated stent and its preparation method. More specifically, it relates to a gold-plated stent that is coated with various chemical materials such as 2-aminoalkanethiol, epihalogenhydrin, and diamine compounds in a sequence and also oligonucleotide gold-plated stent, which is prepared by binding oligonucleotide, a biomaterial, to the gold-plated stent coated with said chemicals. The oligonucleotide gold-plated stent of the present invention has an advantages of raising the local concentration in injured parts and minimizing the toxicity overall the body, so it can be used for prevention of restenosis after angioplasty.

Abstract: Provided is a method of purifying oil by which nano particles are effectively removed from the oil.

Abstract: There is provided a sample preconcentrator. The sample preconcentrator in which a sample gas injection port is coupled to a dried gas supply source and a gas analysis system to concentrate a sample gas comprises a sample concentrating unit containing an absorbent that is composed of carbon nanotube-metal nanocomplexes; a conduit switching valve for selectively coupling the sample gas injection port to the dried gas supply source and the gas analysis system and controlling the absorption and desorption of the sample gas from the sample concentrating unit; and a plurality of conduits for connecting the sample gas injection port, the dried gas supply source, the gas analysis system, the sample concentrating unit and the conduit switching valve.

Abstract: Provided are an apparatus for integrated real-time nucleic acid analysis and a method for detecting target a nucleic acid using the same, and more particularly an integrated real-time nucleic acid analysis for simultaneously performing qualitative analysis or quantitative analysis on genes from various kinds of plural biological samples and a method for detecting target a nucleic acid using the same. The apparatus for integrated real-time nucleic acid analysis and the method for detecting target a nucleic acid using the same according to the present invention, perform tests of various targets required from various samples through a single step promptly and accurately, and thus, can be efficiently used by hospitals or the like needing to rapidly diagnose diseases.

Abstract: Provided are an RNase activity inhibitory compound to effectively control the activity of the RNase promoting degradation of extracted RNAs and, in addition, a sample storage container including the same. The RNase activity inhibitory compound and the sample storage container according to the present invention may be effectively used to store RNAs during RNA extraction or the extracted RNAs.

Abstract: Provided are an siRNA-polymer conjugate, and a method for preparing the same, and more specifically, to a hybrid conjugate formed by covalently bonding siRNA and a polymeric compound for improving the in vivo stability of siRNA, and to a preparation method of the hybrid conjugate. The conjugate of the present invention can improve the in vivo stability of siRNA, thereby achieving an efficient delivery of therapeutic siRNA into cells and exhibiting the activity of siRNA even with a small dose of a relative low concentration. Therefore, the conjugate can advantageously be used as not only an siRNA treatment tool for cancers and other infectious disease, but also a novel type siRNA delivery system.

Abstract: The present invention relates to an apparatus for real-time monitoring chemical reaction between various biomaterials. More particularly, the present invention directed to a real-time monitoring apparatus for biochemical reaction, which comprises parabolic mirror and/or an optical waveguide tube for effective irradiation of light over the whole plate with uniform intensity.

Abstract: Provided is a carbon nanostructure-metal composite nanoporous film in which a carbon nanostructure-metal composite is coated on one surface or both surfaces of a membrane support having micro- or nano-sized pores. A method for manufacturing a carbon nanostructure-metal composite nanoporous film, includes: dispersing a carbon nanostructure-metal composite in a solvent at the presence of a surfactant and coating the carbon nanostructure-metal composite on one surface or both surfaces of a membrane support; and fusing the metal on the membrane support by heating the coated membrane support. The metal in carbon nanostructure-metal composite nanoporous film melts at a low temperature since a size of a metal of the carbon nanostructure-metal composite is several nm to several-hundred nm.

Abstract: Disclosed is a cyclic reverse transcription method, which comprises performing reverse transcription reaction in one or more cycles, each cycle comprising the steps of: (i) performing reaction with a reverse transcription reaction solution comprising template RNA, RNA-dependent DNA polymerase, a reaction buffer, primers for reverse transcription and dNTPs, and optionally, a stabilizer, at 10° C. to 40° C. and, (ii) performing reaction with the resultant reaction solution at 42° to 55° C.

Abstract: A high-throughput device is structured to perform a continuous-flow reaction, e.g., a polymerase chain reaction (PCR) requiring repetitive temperature control in a timely fashion.

Abstract: Disclosed is real-time monitoring apparatus comprising a thermal cycling reaction block having heating block which is formed of a hollow part and divided by an insulating layer, and a capillary tube through which a sample is flowed in and/or out and which is wound on the heating block so that the different temperatures are transferred and thus reaction cycle is repeatedly performed; a light source; a band pass filter; a condensing lens; a beam splitter; a reflecting mirror which is rotatably connected with a motor so as to transfer the excitation light reflected from the beam splitter to the capillary tube and reflect the fluorescence generated from the sample in the capillary tube; and a fluorescence detecting part.

Abstract: The present invention relates to a method for preparing a nano-composite comprising carbon nanotube and metal, more precisely a method for preparing a carbon nanotube-metal composite comprising the steps of preparing a dispersion solution by dispersing carbon nanotube in a reductive solvent; preparing a mixed solution by adding a stabilizer and a metal precursor; and reducing the metal precursor by heating the mixed solution, and a carbon nanotube-metal composite prepared by the same. The method of the present invention favors the production of a carbon nanotube-metal composite which is characterized by even metal particles from some nm to hundreds nm in size and even dispersion of those particles to be bound to carbon nanotube.

Abstract: The present invention relates to silica magnetic particles having a spherical form and a process for preparing the same. The silica magnetic particles prepared according to the present invention, which are silica particles that includes the magnetic particles and additionally have the functional group on the surfaces, has an advantage that the particle size distribution is uniform. Further, the silica magnetic particles prepared according to the present invention can be used as a reagent for separating biomaterials and a reagent for detecting biomaterials.

Abstract: The present invention relates to an automatic refining apparatus for separating target materials from a plurality of biological sample solutions by using magnetic particles to which the magnetic particles are to be reversibly coupled, and to a multi-well plate kit for use in the automatic refining apparatus. Further, the present invention relates to a method for extracting nucleic acids from biological samples by using the above-described automatic refining apparatus. The present invention can be used in the automatic separation of nucleic acid, protein, and the like from biological samples.

Abstract: The present invention relates to primers for PCR amplification comprising abasic parts within the primer sequences and a method for PCR amplification using the same. More precisely, the present invention relates to primers capable of amplifying different templates and having abasic parts complementary to mutated site or polymorphic site of template DNA and a method for PCR amplification comprising the steps of mixing the composition for PCR amplification comprising the primers with nucleic acid template; and performing PCR with the mixture. The primers for PCR amplification of the present invention contain abasic parts not having specific coding information in their nucleotide sequences, so that they can amplify different templates having mutated sites at the same time.

Abstract: The present invention relates to a micro-chamber plate and a manufacturing method of the same, more precisely a micro-chamber plate facilitating real-time measurement and analysis of fluorescence obtained from the reaction of multiple reaction solutions containing primers or probes selectively binding to each corresponding gene without cross-contamination in order to analyze biological samples containing numbers of genes and a manufacturing method of the same.

Abstract: The present invention relates to a dried composition for hot-start PCR, more precisely a dried composition for hot-start PCR with improved stability and long-term storagability which is characteristically prepared by the steps of preparing a reaction mixture by mixing an aqueous solution containing reaction buffer, MgCl2, 4 types of dNTPs, DNA polymerase with pyrophosphate and pyrophosphatase in a reaction tube; and drying the reaction mixture prepared above, a preparation method of the same and a method for amplifying nucleic acid using the same. The dried composition for hot-start PCR is added with pyrophosphate and pyrophosphatase together before drying, so that it can have improved stability and long-term storagability as well as convenience in use, compared with the conventional compositions for hot-start PCR. Therefore, this composition can be effectively used for hot-start PCR, multiplex PCR or real-time quantitative PCR.