Abstract: The present invention relates to a kit for separating and purifying nucleic acids or a variety of biological materials and a system for automatically performing the operations of separating and purifying a variety of biological materials using the kit. The kit of the present invention comprises a container with a plurality of chambers formed in a row for containing buffers and solid materials suitable for separating the nucleic acids or biological materials from the biological samples, and a carriage including a fiat portion with a hole formed therethrough and a projection having an inside passage of which one end is closed and the other end is open and having a predetermined length such that the projection can be dipped into the buffers. A mounting means is formed on the flat portion of the carriage such that the carriage can be detachably mounted to a transport means for transferring the carriage to the respective chambers.