Abstract: The invention provides a genetically modified bacterium for production of thiamine; where the bacterium is characterized by a transgene encoding a thiamine monophosphate phosphatase (TMP phosphatase having EC 3.1.3.-) as well as transgenes encoding polypeptides that catalyze steps in the thiamine pathway. The genetically modified bacterium is characterized by enhanced synthesis and release of thiamine into the extracellular environment. The invention further provides a method for producing thiamine using the genetically modified bacterium of the invention; as well as the use of the genetically modified bacterium for extracellular thiamine production.
Type:
Grant
Filed:
December 16, 2016
Date of Patent:
June 30, 2020
Assignee:
BIOSYNTHIA APS
Inventors:
Luisa Gronenberg, Bo Salomonsen, Matteo Ferla, Hans Jasper Genee