Abstract: Embodiments of the present application relate to functionalized N-acetylgalactosamine-analogs, methods of making, and uses of the same. In particular, mono or trivalent N-acetylgalactosamine analogs may be prepared by utilizing a wide variety of linkers containing functional groups. These functionalized N-acetylgalactosamine-analogs may be used in the preparation of targeted delivery of oligonucleotide-based therapeutics.

Abstract: The invention relates to a surface gradient cross-linked method of a ultra-high molecular weight polyethylene and the application thereof. The surface gradient crosslinked method of ultrahigh molecular weight polyethylene includes a n ultra-high molecular weight polyethylene surface photoinitiator diffusion step and an ultraviolet light irradiation crosslinking step. In this method, the photoinitiator is diffused deeply into the surface of ultra-high molecular weight polyethylene, the body material shows a gradient cross-linking from the surface to the inside after ultraviolet irradiation so that the whole bulk has a high toughness and the surface there of has a high wear resistance.

Abstract: Embodiments of the present application relate to the preparation of 2?-O-protected nucleoside phosphoramidites and conjugation of the 2?-O-protected nucleoside to a solid support. More specifically, the present application relates to nucleosides having a hydroxy protecting group at the 2? position that reduces migration to the 3? position during RNA (e.g., mRNA) synthesis and can be readily removed under mild conditions without the use of toxic metal-containing reagents. Methods of using the nucleosides described herein in RNA synthesis are also disclosed.

Abstract: Embodiments of the present application relate to functionalized N-acetylgalactosamine-analogs, methods of making, and uses of the same. In particular, mono or trivalent N-acetylgalactosamine analogs may be prepared by utilizing a wide variety of linkers containing functional groups. These functionalized N-acetylgalactosamine-analogs may be used in the preparation of targeted delivery of oligonucleotide-based therapeutics.

Abstract: A reloadable medicine injector and methods are described in which a barrel with a receiving cavity is adapted to slidably receive a syringe subassembly for axial movement therein. Upon removal of a safety and release of a syringe driver, the syringe driver moves forward and injects the syringe needle. A plurality of penetration controls are shown for controlling injection needle penetration depth. The penetration controls have an abutment and various lengths to provide different needle penetration depth positions. In one form of penetration control a sleeve is used against which the syringe or related parts contact. In another form the front return spring is used as a penetration control. A cushioning ring may be used to reduce syringe breakage. A load distribution and guide ring may be used to distribute loading applied to the syringe and help guide the moving syringe.

Abstract: A reloadable medicine injector and methods are described in which a barrel with a receiving cavity is adapted to slidably receive a syringe subassembly for axial movement therein. Upon removal of a safety and release of a syringe driver, the syringe driver moves forward and injects the syringe needle. A plurality of penetration controls are shown for controlling injection needle penetration depth. The penetration controls have an abutment and various lengths to provide different needle penetration depth positions. In one form of penetration control a sleeve is used against which the syringe or related parts contact. In another form the front return spring is used as a penetration control. A cushioning ring may be used to reduce syringe breakage. A load distribution and guide ring may be used to distribute loading applied to the syringe and help guide the moving syringe.

Abstract: Embodiments of the present application relate to polymers used as polymeric polyvalent hub for liquid phase oligonucleotide synthesis. Methods for making an oligonucleotide by liquid phase oligonucleotide synthesis using the polyvalent hub are also provided.

Abstract: Embodiments of the present application relate to porous polymeric beads solid support for use in oligonucleotide synthesis and the method of producing and using the same. Further embodiments relate to porous polymeric beads and method for preparing and using the same.

Abstract: A method of making an implant having a porous portion is disclosed. The method comprises the following steps: obtaining an artificial foam containing porous portion; scanning the artificial foam to obtain a digital porous model; editing the digital porous model; assembling the digital porous model to form a digital porous block; editing the digital porous block to obtain a digital implant model; forming the implant by printing the digital implant model through a 3D printer. An implant and a method of calculating porosity a porosity of a porous material are also disclosed.

Abstract: Embodiments of the present application relate to polymers for liquid phase oligonucleotide synthesis. Methods for making an oligonucleotide by liquid phase oligonucleotide synthesis, comprising dissolving a polyvalent hub having a plurality of functional groups in a solvent and contacting the polyvalent hub with one or more nucleoside analogs to form a first bioconjugate are also provided.

Abstract: Embodiments of the present application relate to functionalized N-acetylgalactosamine-analogs, methods of making, and uses of the same. In particular, mono or trivalent N-acetylgalactosamine analogs may be prepared by utilizing a wide variety of linkers containing functional groups. These functionalized N-acetylgalactosamine-analogs may be used in the preparation of targeted delivery of oligonucleotide-based therapeutics.

Abstract: Embodiments of the present application relate to N-acetylgalactosamine-conjugated nucleosides. In particular, the N-acetylgalactosamine is installed on the nucleobase of the nucleosides through a wide variety of linkers. Methods of making N-acetylgalactosamine-conjugated nucleosides are also disclosed herein. N-acetylgalactosamine is a well-defined liver-targeted moiety and N-acetylgalactosamine-conjugated nucleosides may be used in the preparation of targeted delivery of oligonucleotide-based therapeutics.

Abstract: Provided herein are devices, methods and compositions useful in obtaining aptamers for biosensor probes. Such methods, devices and compositions are useful for novel clinical or companion drug diagnostic and personalized theranostic assays.

Abstract: A method for detecting a SNP site on a SMA gene is disclosed, and includes steps of: (S10) performing a PCR for amplifying a nucleic a nucleic acid fragment containing a SNP site; (S20) performing a dephosphorylation reaction on the nucleic acid fragment; (S30) performing an extension reaction on the nucleic acid fragment, wherein the SNP site is identified by using an extension primer, a 3?-end of the extension primer is extended by a single nucleotide which is complementary to a base of the SNP site, and thus an extended extension primer is obtained; (S40) performing a purification reaction; and (S50) measuring a molecular weight of the extended extension primer, and determining a type of a base of the single nucleotide based upon the molecular weight, thereby determining whether deletion occurs to the SNP site.

Abstract: Provided are a screw driver system and a screw driving bit. The screw driving bit includes a body having a first end and a second end with a longitudinal axis defined therebetween and an outer surface between the first and second ends. The screw driving bit includes a first circumferential groove and a second circumferential groove that are affixed in coaxially positioned arrangement about the longitudinal axis on the outer surface of the body each having a bottom surface. The bottom surface of the first circumferential groove defines a first outer diameter that is larger than a second outer diameter defined by the second circumferential groove. The guide clamp includes a jaw for holding the screw driving bit. When engaged with the first circumferential groove, the guide clamp prevents rotation of the screw driving bit. When engaged with the second circumferential groove, the guide clamp does not prevent rotation of the screw driving bit.

Abstract: Provided are a screw driver system and a screw driving bit. The screw driving bit includes a body having a first end and a second end with a longitudinal axis defined therebetween and an outer surface between the first and second ends. The screw driving bit includes a first circumferential groove and a second circumferential groove that are affixed in coaxially positioned arrangement about the longitudinal axis on the outer surface of the body each having a bottom surface. The bottom surface of the first circumferential groove defines a first outer diameter that is larger than a second outer diameter defined by the second circumferential groove. The guide clamp includes a jaw for holding the screw driving bit. When engaged with the first circumferential groove, the guide clamp prevents rotation of the screw driving bit. When engaged with the second circumferential groove, the guide clamp does not prevent rotation of the screw driving bit.

Abstract: A method for preparing a tea leaf extract is used for preparing the tea leaf extract with improved anti-oxidation activity. The method includes smoldering a rice husk sample at 200-400° C. in a low oxygen environment for 2-4 hours, followed by burning at 400-600° C. in an atmospheric environment for 2-4 hours to obtain a rice husk silica. An oxygen concentration in the low oxygen environment is below 5%. The rice husk silica is dissolved in an alkaline solution to obtain a rice husk silica solution. A tea leaf sample is then extracted by the rice husk silica solution.

Abstract: A screw driver system includes a screw driving bit and a guide clamp. The screw driving bit includes a unitary body having a first end and a second end with a longitudinal axis defined therebetween and an outer surface having a cylindrical portion between the first and second ends. The screw driving bit includes a first circumferential groove and a second circumferential groove that are affixed in coaxially positioned arrangement about the longitudinal axis on the outer surface of the cylindrical portion each having a bottom surface. The bottom surface of the first circumferential groove defines a first outer diameter that is larger than a second outer diameter defined by the second circumferential groove. The guide clamp includes a jaw for holding the screw driving bit. When engaged with the first circumferential groove, the guide clamp prevents rotation of the screw driving bit. When engaged with the second circumferential groove, the guide clamp does not prevent rotation of the screw driving bit.

Abstract: A screw driver system includes a screw driver and a guide clamp. The screw driver includes a driving bit. The driving bit includes a body having a first end and a second end and an outer surface comprising a cylindrical portion between the first and second ends. The screw driving bit includes a first circumferential groove and a second circumferential groove on the outer surface of the cylindrical portion each having a bottom surface. The bottom surface of the first circumferential groove defines a first outer diameter that is larger than a second outer diameter defined by the second circumferential groove. The guide clamp includes a jaw for holding the screw driving bit. When engaged with the first circumferential groove, the guide clamp prevents rotation of the screw driving bit. When engaged with the second circumferential groove, the guide clamp does not prevent rotation of the screw driving bit.

Abstract: A patellar implant includes a body and at least one oblique anchoring pin. The body includes a bone-facing surface that is substantially planar and has a lateral end and a medial end. The body also includes an articulating surface opposite the bone-facing surface. The at least one oblique anchoring pin extends from the bone-facing surface at an acute angle toward the lateral end of the bone-facing surface. The at least one oblique anchoring pin includes a first end attached to the bone-facing surface and a second end spaced from the first end. A longitudinal axis extends from the first end to the second end. The longitudinal axis defines the acute angle with respect to the bone-facing surface. The at least one oblique anchoring pin extends into a surgically-prepared patella when the patellar implant is such that the surgically-prepared patella abuts the bone-facing surface.