Patents Assigned to BIOTECHNOLOGY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCES
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Patent number: 11879131Abstract: A use of a ZmSBP12 gene in the regulation of drought resistance, plant height, and ear height of Zea mays L. is provided. After the ZmSBP12 gene is over-expressed in Zea mays L., the resulting Zea mays L. mutant plant exhibits increased drought resistance and decreased plant and ear heights. The overexpression of the ZmSBP12 gene leads to increased drought resistance and decreased plant and ear heights, indicating that the ZmSBP12 gene plays a crucial role in the drought resistance and plant type (plant height) of Zea mays L. The expression abundance of the ZmSBP12 gene is increased to improve the drought resistance of Zea mays L. and reduce the plant and ear heights of Zea mays L., which can be used for the assisted breeding of novel drought-resistant and lodging-resistant Zea mays L. varieties and the breeding of excellent inbred lines and hybrids of Zea mays L.Type: GrantFiled: October 21, 2020Date of Patent: January 23, 2024Assignees: SOUTH CHINA AGRICULTURAL UNIVERSITY, BIOTECHNOLOGY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Haiyang Wang, Yurong Xie, Bingbing Zhao, Baobao Wang, Yongping Zhao, Dexin Kong, Quanquan Li, Yaoyao Li
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Publication number: 20230227827Abstract: An artificial non-coding RNA (ncRNA) module constructed by a synthetic biology technique and the use of the artificial ncRNA module in the construction of an artificial nitrogen fixation system are disclosed. The RNA module can enhance the post-transcriptional stability of nifHDK mRNA by interacting with a nitrogenase coding gene nifHDK mRNA, thereby improving the nitrogen fixation ability of a chassis microorganism. A fusion expression vector carrying the artificial RNA module is constructed and transformed into different chassis nitrogen-fixing microorganisms. It is confirmed through experiments that, under nitrogen fixation conditions, the artificial RNA module of the present disclosure can significantly improve the nitrogenase activity of a recombinant engineering bacterial strain.Type: ApplicationFiled: July 6, 2020Publication date: July 20, 2023Applicant: BIOTECHNOLOGY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Min LIN, Yuhua ZHAN, Yongliang YAN, Xiubin KE
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USE OF ZmSBP12 GENE IN REGULATION OF DROUGHT RESISTANCE, PLANT HEIGHT, AND EAR HEIGHT OF ZEA MAYS L.
Publication number: 20230117599Abstract: A use of a ZmSBP12 gene in the regulation of drought resistance, plant height, and ear height of Zea mays L. is provided. After the ZmSBP12 gene is over-expressed in Zea mays L., the resulting Zea mays L. mutant plant exhibits increased drought resistance and decreased plant and ear heights. The overexpression of the ZmSBP12 gene leads to increased drought resistance and decreased plant and ear heights, indicating that the ZmSBP12 gene plays a crucial role in the drought resistance and plant type (plant height) of Zea mays L. The expression abundance of the ZmSBP12 gene is increased to improve the drought resistance of Zea mays L. and reduce the plant and ear heights of Zea mays L., which can be used for the assisted breeding of novel drought-resistant and lodging-resistant Zea mays L. varieties and the breeding of excellent inbred lines and hybrids of Zea mays L.Type: ApplicationFiled: October 21, 2020Publication date: April 20, 2023Applicants: SOUTH CHINA AGRICULTURAL UNIVERSITY, BIOTECHNOLOGY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Haiyang WANG, Yurong XIE, Bingbing ZHAO, Baobao WANG, Yongping ZHAO, Dexin KONG, Quanquan LI, Yaoyao LI -
Publication number: 20220396805Abstract: A key DNA sequence for regulating a maize leaf angle and a mutant thereof are provided, which have polynucleotide sequences shown in SEQ ID No. 1 and SEQ ID No. 2, respectively. The DNA sequence for regulating a maize leaf angle and the mutant thereof provided by present disclosure can regulate the expression of ZmNAC16 gene in a maize pulvinus, and thus can be used for the improvement of maize leaf angle and plant type and further for the cultivation of new maize varieties. The present disclosure further provides specific detection primers for detecting mutations of the DNA key sequence and the mutant, and detection primers for detecting an expression level of ZmNAC16 gene in maize. These detection primers can be used to directionally improve a maize leaf angle and also shows application potential for breeding of dense-planting-tolerant and high-yield maize.Type: ApplicationFiled: October 21, 2020Publication date: December 15, 2022Applicants: SOUTH CHINA AGRICULTURAL UNIVERSITY, BIOTECHNOLOGY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Haiyang WANG, Baobao WANG, Yurong XIE, Xing LI, Bingbing ZHAO, Yongping ZHAO
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Patent number: 10738314Abstract: Provided is an amino acid optimization of a functional motif on an IrrE protein of a Deinococcus geothermalis strain and homologous proteins thereof obtained by site mutation, wherein a second-site or fifth-site alanine in a functional domain motif 154LAELAR159 is mutated. into serine.Type: GrantFiled: May 9, 2016Date of Patent: August 11, 2020Assignee: BIOTECHNOLOGY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Wei Zhang, Zhengfu Zhou, Ming Chen, Min Lin
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Patent number: 10053682Abstract: The present invention relates to the field of genetic engineering and hereditary engineering. The present invention discloses a ?-galactosidase (?-galactoside galactohydrolase, EC 3.2.1.23) mutant with high transglycosidase activity, which is obtained by single-site-saturation mutation of amino acid sequences of ?-galactosidase from Aspergillus candidus and Aspergillus oryzae, with own signal peptides removed. The transglycosidase activity of the mutant is over 15% higher than that of wild types. Meanwhile, the present invention also discloses a DNA molecule which encodes the mutant, a recombinant expression vector containing the DNA molecule, and a host cell expressing the DNA molecule. In addition, the present invention also provides a method for preparing ?-galactosidase mutant with high transglycosidase activity by using the recombinant expression vector and applications of the mutant, the DNA molecule, the recombinant expression vector and the host cell in preparation of ?-galactosidase.Type: GrantFiled: April 7, 2015Date of Patent: August 21, 2018Assignee: BIOTECHNOLOGY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Wei Zhang, Yuhong Zhang, Bo Liu, Ning Sun, Jialin Zhang
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Publication number: 20180163215Abstract: Provided is an amino acid optimization of a functional motif on an IrrE protein of a Deinococcus geothermalis strain and homologous proteins thereof obtained by site mutation, wherein a second-site or fifth-site alanine in a functional domain motif 154LAELAR159 is mutated into serine.Type: ApplicationFiled: May 9, 2016Publication date: June 14, 2018Applicant: BIOTECHNOLOGY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Wei ZHANG, Zhengfu ZHOU, Ming CHEN, Min LIN
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Publication number: 20170204389Abstract: The present invention relates to the field of genetic engineering and hereditary engineering. The present invention discloses a ?-galactosidase (?-D-galactoside galactohydrolase, EC 3.2.1.23) mutant with high transglycosidase activity, which is obtained by single-site-saturation mutation of amino acid sequences of ?-galactosidase from Aspergillus candidus and Aspergillus oryzae, with own signal peptides removed. The transglycosidase activity of the mutant is over 15% higher than that of wild types. Meanwhile, the present invention also discloses a DNA molecule which encodes the mutant, a recombinant expression vector containing the DNA molecule, and a host cell expressing the DNA molecule. In addition, the present invention also provides a method for preparing ?-galactosidase mutant with high transglycosidase activity by using the recombinant expression vector and applications of the mutant, the DNA molecule, the recombinant expression vector and the host cell in preparation of ?-galactosidase.Type: ApplicationFiled: April 7, 2015Publication date: July 20, 2017Applicant: BIOTECHNOLOGY RESEARCH INSTITUTE, CHINESE ACADEMY OF AGRICULTURAL SCIENCESInventors: Wei ZHANG, Yuhong ZHANG, Bo LIU, Ning SUN, Jialin ZHANG
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Patent number: 8207403Abstract: An EPSP synthase (5-enolpyruvylshikimate-3-phosphate synthase) with high glyphosate resistance and a nucleotide sequence encoding the synthase are disclosed. The gene encoding the EPSP synthase has low homology with the reported EPSP synthase. A transgenic plant obtained by the expression of the gene in plant has an increased resistance to glyphosate after experimental confirmation.Type: GrantFiled: November 16, 2007Date of Patent: June 26, 2012Assignee: Biotechnology Research Institute The Chinese Academy of Agricultural SciencesInventors: Min Lin, Aimin Liang, Wei Lu, Liang Li, Ming Chen, Wei Zhang, Shuzhen Ping
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Patent number: 8153861Abstract: A gene comprising the nucleic acid sequence of SEQ ID NO:1 and another gene comprising the nucleic acid sequence of SEQ ID NO:2, the latter being artificially synthesized according to plant preferred codons. Both genes encode a protein having the amino acid sequence of SEQ ID NO:3. Also provided are recombinant vectors containing each of the genes and host cells transformed with the recombinant vectors. The host cells can be prokaryotic cells [[and]] or eukaryotic cells. The transgenic plants comprising the gene having the nucleic acid sequence of SEQ ID NO:2 show improved salt and drought tolerance after the [[said]] gene is expressed in the transgenic plants.Type: GrantFiled: November 9, 2007Date of Patent: April 10, 2012Assignee: Biotechnology Research Institute, The Chinese Academy of Agricultural SciencesInventors: Min Lin, Ming Chen, Jin Wang, Jie Pan, Zhengfu Zhou, Wei Zhang, Wei Lu, Shuzhen Ping
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Publication number: 20110196142Abstract: DNA molecule for expressing hairpin RNA, the constructing method and the use thereof are provided. The DNA molecule is a closed single-strand cyclic DNA molecule which is constructed by linking four fragments A, B, C and D as following order: A-C-B-D or A-D-B-C, wherein A is a sense strand or anti-sense strand fragment of the target double-strand DNA; B is the fragment which is reversely complementary to A; C and D are DNA fragments with stem-loop structure and they meet one of the following requirements: 1) the nucleotide sequence of fragment C or D comprises at least one sequence e; 2) the nucleotide sequence of fragment C comprises at least one sequence e, the nucleotide sequence of fragment D comprises at least one sequence f, wherein sequences e and f individually are one strand of different restriction endonuclease recognition sequences. The DNA molecule for expressing hairpin RNA and its constructing method are useful in constructing lhRNA library.Type: ApplicationFiled: September 4, 2008Publication date: August 11, 2011Applicant: Biotechnology Research Institute The Chinese Academy of Agricultural SciencesInventors: Lei Wang, YunLiu Fan
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Publication number: 20110173716Abstract: An EPSP synthase (5-enolpyruvylshikimate-3-phosphate synthase) with high glyphosate resistance and a nucleotide sequence encoding the synthase are disclosed. The gene encoding the EPSP synthase has low homology with the reported EPSP synthase. A transgenic plant obtained by the expression of the gene in plant has an increased resistance to glyphosate after experimental confirmation.Type: ApplicationFiled: November 16, 2007Publication date: July 14, 2011Applicant: Biotechnology Research Institute The chinese Academy of Agricultural SciencesInventors: Min Lin, Aimin Liang, Wei Lu, Liang Li, Ming Chen, Wei Zhang, Shuzhen Ping
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Publication number: 20110088121Abstract: Provide a nucleotide coding sequence and another nucleotide coding sequence artificially synthesized according to biased codons of plant. Construct recombinant vectors containing the genes as above and transform them into host cells including prokaryotic cells and eukaryotic cells. It is confirmed that the resulting transgenic plant has improved salt and drought tolerance after the said genes are expressed in the plant.Type: ApplicationFiled: November 9, 2007Publication date: April 14, 2011Applicant: Biotechnology Research Institute The Chinese Academy of Agricultural SciencesInventors: Min Lin, Ming Chen, Jin Wang, Jie Pan, Zhengfu Zhou, Wei Zhang, Wei Lu, Shuzhen Ping