Abstract: The invention is related to the process of fermentation with production strain Bacillus polymyxa for industrial production of antibiotic polymyxin B with yield from 1.8 to 2.8 g/l of filtrate of fermentation broth at temperature 28° C., pressure 40?70 kPa, aeration from 0.4 to 1 vvm, concentration of dissolved oxygen minimal 60%, maintenance of pH and optimal concentration of glucose and ammonium ions.
Type:
Grant
Filed:
May 21, 2007
Date of Patent:
February 21, 2012
Assignee:
Biotika A.S.
Inventors:
Ivan Varga, Katarina Vrúblová, Ludmila Kovácová, Jana Kluková
Abstract: The invention is related to the method of polymyxine B recovery from fermentation broth for the purpose of pure substance recovery. The invention mentioned above is obtained by using the method of polymyxine B recovery from fermentation broth according invention, which abstract is characterized by, that the filtrate is obtained from fermentation broth, its pH and color is adjusted and purified on non-ionogenic synthetic adsorbents of polystyrene type with specific surface 500 till 1000 m2·g?1 and fabric size 3 till 30 nm. The polymyxine B is eluated from adsorbent by aqueous solution of organic solvent. The polymyxine B base is coagulated from eluate after adjustment of pH to alcaline area. The polymyxine B base is converted by mineral acid to polymyxine B salt solution, from which is obtained crystal substance by drying.
Type:
Grant
Filed:
May 11, 2007
Date of Patent:
May 31, 2011
Assignee:
Biotika A.S.
Inventors:
Ivan Varga, Mária Bobálová, Eva Michalková, Mária Jakubcová