Abstract: A holding device for body fluid, tissue parts and tissue culture media includes a cylindrical holding container having a container wall with an inner surface defining an inner space, a first open end face having a diameter which is at least equal to the diameter of the inner space, and a second open end face having a conical sealing surface tapering inwardly in the direction of the inner space. A first closure device closes the first open end face and a second closure device closes the second end face. The second closure device includes a solid conical seal body 44 having a sealing surface conforming to the conical sealing surface of the second open end face and an outer end face of a larger diameter than that of an inner end face that faces the inner space. A retaining member 45 abuts the outer end face and is coupled to the outer container wall surface for holding the seal body in a sealing position in the second open end face.