Patents Assigned to Calgene LLC
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Patent number: 8722384Abstract: The present invention relates to fatty acid desaturases able to catalyze the conversion of oleic acid to linoleic acid, linoleic acid to ?-linolenic acid, or of alpha-linolenic acid to stearidonic acid. Nucleic acid sequences encoding desaturases, nucleic acid sequences which hybridize thereto, DNA constructs comprising a desaturase gene, and recombinant host microorganism or animal expressing increased levels of a desaturase are described. Methods for desaturating a fatty acid and for producing a desaturated fatty acid by expressing increased levels of a desaturase are disclosed. Fatty acids, and oils containing them, which have been desaturated by a desaturase produced by recombinant host microorganisms or animals are provided. Pharmaceutical compositions, infant formulas or dietary supplements containing fatty acids which have been desaturated by a desaturase produced by a recombinant host microorganism or animal also are described.Type: GrantFiled: March 30, 2009Date of Patent: May 13, 2014Assignees: Calgene LLC, Abbott LaboratoriesInventors: Deborah Knutzon, Pradip Mukerji, Yung-Sheng Huang, Jennifer Thurmond, Sunita Chaudhary, Amanda Eun-Yeong Leonard
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Publication number: 20110256260Abstract: The present invention relates to fatty acid desaturases able to catalyze the conversion of oleic acid linoleic acid, linoleic acid to ?-linolenic acid, or of alpha-linolenic acid to stearidonic acid. Nucleic acid sequences encoding desaturases, nucleic acid sequences which hybridize thereto, DNA constructs comprising a desaturase gene, and recombinant host microorganism or animal expressing increased levels of a desaturase are described. Methods for desaturating a fatty acid and for producing a desaturated fatty acid by expressing increased levels of a desaturase are disclosed. Fatty acids, and oils containing them, which have been desaturated by a desaturase produced by recombinant host microorganisms or animals are provided. Pharmaceutical compositions, infant formulas or dietary supplements containing fatty acids which have been desaturated by a desaturase produced by a recombinant host microorganism or animal also are described.Type: ApplicationFiled: March 30, 2009Publication date: October 20, 2011Applicants: Calgene LLC, Abbott LaboratoriesInventors: Deborah Knutzon, Pradip Mukerji, Yung-Sheng Huang, Jennifer Thurmond, Sunita Chaudhary, Amanda Leonard
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Patent number: 7812220Abstract: Methods of altering substrate specificity of beta-ketoacyl-ACP synthase, and engineered beta-ketoacyl-ACP synthases so produced are provided. DNA sequences and constructs for expression of engineered beta-ketoacyl-ACP synthases, as well as the novel beta-ketoacyl-ACP synthases produced therefrom are also provided. Such DNA sequences may be used for expression of the engineered beta-ketoacyl-ACP synthases in host cells, particularly seed cells of oilseed crop plants, for the modification of fatty acid composition.Type: GrantFiled: October 23, 2008Date of Patent: October 12, 2010Assignee: Calgene LLCInventors: Katayoon Dehesh, Dale Val
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Patent number: 7732678Abstract: Novel DNA constructs are provided which may be used as molecular probes or inserted into a plant host to provide for modification of transcription of a DNA sequence of interest in cotton fiber, particularly in very early fiber development. The DNA constructs comprise a cotton fiber transcriptional initiation regulatory region associated with a gene which is expressed in cotton fiber.Type: GrantFiled: December 3, 1997Date of Patent: June 8, 2010Assignee: Calgene LLCInventors: Kevin McBride, David M. Stalker, Julie R. Pear, Luis Perez-Grau
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Patent number: 7531347Abstract: The present invention relates to fatty acid desaturases able to catalyze the conversion of oleic acid to linoleic acid, linoleic acid to ?-linolenic acid, or of alpha-linolenic acid to stearidonic acid. Nucleic acid sequences encoding desaturases, nucleic acid sequences which hybridize thereto, DNA constructs comprising a desaturase gene, and recombinant host microorganism or animal expressing increased levels of a desaturase are described. Methods for desaturating a fatty acid and for producing a desaturated fatty acid by expressing increased levels of a desaturase are disclosed. Fatty acids, and oils containing them, which have been desaturated by a desaturase produced by recombinant host microorganisms or animals are provided. Pharmaceutical compositions, infant formulas or dietary supplements containing fatty acids which have been desaturated by a desaturase produced by a recombinant host microorganism or animal also are described.Type: GrantFiled: April 10, 1998Date of Patent: May 12, 2009Assignees: Abbott Laboratories, Calgene, LLCInventors: Deborah Knutzon, Pradip Mukerji, Yung-Sheng Huang, Jennifer Thurmond, Sunita Chaudhary, Amanda Eun-Yeong Leonard
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Patent number: 7420101Abstract: Nucleic acid sequences and methods are provided for producing plants and seeds having altered tocopherol content and compositions. The methods find particular use in increasing the tocopherol levels in plants, and in providing desirable tocopherol compositions in a host plant cell.Type: GrantFiled: March 29, 2005Date of Patent: September 2, 2008Assignee: Calgene LLCInventors: Sai S. Subramaniam, Steven C. Slater, Katherine Karberg, Ridong Chen, Henry E. Valentin, Yun-Hua Huang Wong
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Patent number: 7371924Abstract: This invention relates to sequences encoding ?-ketoacyl-ACP synthase (KAS) and methods of use thereof. Also provided are methods for decreasing saturated fatty acid levels as a component of total triglycerides found in plant oils. The method generally comprises growing a soybean plant having integrated into its genome a DNA construct comprising, in the 5? to 3? direction of transcription, a promoter functional in a soybean plant seed cell, a DNA sequence encoding a KAS protein, and a transcription termination region functional in a plant cell. The present invention also provides a soybean seed with less than about 3.5 weight percent total saturated fatty acids.Type: GrantFiled: December 12, 2003Date of Patent: May 13, 2008Assignee: Calgene LLCInventor: Katayoon Dehesh
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Patent number: 7335815Abstract: Methods are provided for producing plants and seeds having altered isoprenoid content and compositions. The methods find particular use in increasing the isoprenoid levels in plants, and in providing desirable isoprenoid compositions in a host plant cell.Type: GrantFiled: October 15, 2004Date of Patent: February 26, 2008Assignee: Calgene LLCInventors: Albert Boronat, Narciso Campos, Ganesh M. Kishore
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Patent number: 7332311Abstract: By this invention, nucleic acid sequences encoding for fatty acyl-CoA:fatty alcohol acyltransferase (wax synthase) are provided, wherein said wax synthase is active in the formation of a wax ester from fatty alcohol and fatty acyl-CoA substrates. Of special interest are nucleic acid sequences obtainable from a jojoba embryo wax synthase having an apparent molecular mass of approximately 33 kD. Also considered are amino acid and nucleic acid sequences obtainable from wax synthase proteins and the use of such sequences to provide transgenic host cells capable of producing wax esters.Type: GrantFiled: April 18, 2003Date of Patent: February 19, 2008Assignee: Calgene LLCInventors: Kathryn D. Lardizabal, James G. Metz, Michael W. Lassner
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Patent number: 7301070Abstract: By this invention, compositions and methods of use related to ?-ketoacyl-ACP synthase Of special interest are synthases obtainable from Cuphea species. Amino acid and nucleic acid for synthase protein factors are provided, as well as methods to utilize such sequences in constructs for production of genetically engineered plants having altered fatty acid compositions. Of particular interest is the expression of synthase protein factors in conjunction with expression of plant medium-chain acyl-ACP thioesterases for production of increased levels and/or modified ratios of medium-chain fatty acids in oils of transgenic plant seeds.Type: GrantFiled: August 7, 2003Date of Patent: November 27, 2007Assignee: Calgene LLCInventor: Katayoon Dehesh
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Patent number: 7265207Abstract: Nucleic acid sequences and methods are provided for producing plants and seeds having altered tocopherol content and compositions. The methods find particular use in increasing the tocopherol levels in plants, and in providing desirable tocopherol compositions in a host plant cell.Type: GrantFiled: January 23, 2003Date of Patent: September 4, 2007Assignee: Calgene LLCInventors: Michael W. Lassner, Beth Savidge, Timothy A. Mitsky, James D. Weiss, Martha Ann Post-Beittenmiller
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Patent number: 7259293Abstract: Constructs and methods are provided for expressing peptides derived from eukaryotic organisms in plant plastids. Constructs have a promoter functional in a plant plastid, a DNA sequence encoding a peptide derived from an eukaryotic organism and a transcription termination region. Other elements include a selectable marker for selection of plant cells comprising a plastid expressing the marker and DNA regions of homology to the genome of the plastid and optionally a ribosome binding site joined to the promoter. By methods using such constructs high levels of eukaryotic peptides, such as mammalian proteins, are produced in a plant cell by growing plant cells under conditions whereby the DNA encoding sequences are expressed to produce eukaryotic peptide in said plastid.Type: GrantFiled: August 13, 2004Date of Patent: August 21, 2007Assignee: Calgene LLCInventor: Jeffrey M. Staub
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Patent number: 7256329Abstract: By this invention, novel nucleic acid sequences are provided, wherein said nucleic acid sequence is a genomic sequence of a plant desaturase encoding sequence. Also provided in the present invention are the promoter and intron sequences of the desaturase genomic sequences. Furthermore, recombinant DNA constructs employing the polynucleotide sequences are provided. The instant invention also provides methods for the modification of fatty acid compositions in host plant cells.Type: GrantFiled: June 27, 2003Date of Patent: August 14, 2007Assignee: Calgene LLCInventor: Joanne Fillatti
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Patent number: 7202085Abstract: DNA constructs and other compositions and methods for controlling gene expression in eukaryotic cells and organisms are derived from bacterial quorum sensing systems. One or more cis elements from the luxI promoter (“lux box”) or a functionally similar sequence are incorporated in a eukaryotic promoter. A receptor protein from the LuxR family of transcriptional regulators, upon binding an acylated homoserine lactone (AHL) compound, interacts with the lux box, modulating the activity of the promoter.Type: GrantFiled: June 3, 2005Date of Patent: April 10, 2007Assignee: Calgene LLCInventors: Tim N. Oulmassov, Kevin E. McBride, Paula C. Miller, John C. Anderson, Lyle D. Crossland, Thomas H. Adams, Barbara A. Qurollo, Victoria Gavrias
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Patent number: 7148336Abstract: By this invention, novel nucleic acid sequences are provided, wherein said nucleic acid sequence is a genomic sequence of a plant desaturase encoding sequence. Also provided in the present invention are the promoter and intron sequences of the desaturase genomic sequences. Furthermore, recombinant DNA constructs employing the polynucleotide sequences are provided. The instant invention also provides methods for the modification of fatty acid compositions in host plant cells.Type: GrantFiled: October 11, 2002Date of Patent: December 12, 2006Assignee: Calgene LLCInventor: Joanne Fillatti
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Patent number: 7141718Abstract: Nucleic acid sequences and methods are provided for producing plants and seeds having altered tocopherol content and compositions. The methods find particular use in increasing the tocopherol levels in plants, and in providing desirable tocopherol compositions in a host plant cell.Type: GrantFiled: May 13, 2003Date of Patent: November 28, 2006Assignee: Calgene LLCInventors: Michael W. Lassner, Timothy Mitsky, James Weiss, Martha Ann Post-Beittenmiller
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Patent number: 7135617Abstract: The invention provides diacylglycerol acyltransferase (DAGAT) proteins, wherein said proteins are active in the formation of triacylglycerol from fatty acyl and diacylglycerol substrates. In one aspect, Mortierella ramanniana DAGAT proteins have been isolated and have molecular weights of between approximately 36 and 37 kDa as measured by SDS-PAGE. The invention also provides novel DAGAT polynucleotide and polypeptide sequences and to methods of producing such polypeptides using recombinannt techniques. In addition, methods are provided for using such sequences to alter triacylglycerol levels in plants and to treat diseases associated with altered DAGAT activity or expression.Type: GrantFiled: July 31, 2002Date of Patent: November 14, 2006Assignee: Calgene LLCInventors: Kathryn Dennis Lardizabal, Gregory A. Thompson, Deborah Hawkins
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Patent number: 7067647Abstract: Methods are provided for producing plants and seeds having altered isoprenoid content and compositions. The methods find particular use in increasing the isoprenoid levels in plants, and in providing desirable isoprenoid compositions in a host plant cell.Type: GrantFiled: November 13, 2001Date of Patent: June 27, 2006Assignee: Calgene LLCInventors: Albert Boronat, Narciso Campos, Ganesh M. Kishore
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Patent number: 7053267Abstract: By this invention, modification of the fatty acid composition of a plant seed may be achieved as a result of the activity of a DNA sequence foreign to the plant species to be modified. In particular, it has been found that a plant oil having a modified fatty acid composition can be obtained upon the expression of genes derived from plants of different species than the host plant, upon the expression of genes derived from bacteria, and from the transcription of anti-sense sequences which are complementary to endogenous genes of the plant host cell. In a preferred embodiment, transcription of the fatty acid modifying foreign DNA sequence is restricted to the developing seed tissues.Type: GrantFiled: March 18, 2002Date of Patent: May 30, 2006Assignee: Calgene LLCInventors: Vic C. Knauf, Gregory Thompson
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Patent number: RE41139Abstract: The present invention relates to compositions and methods for preparing polyunsaturated long chain fatty acids in plants, plant parts and plant cells, such as leaves, roots, fruits and seeds. Nucleic acid sequences and constructs encoding fatty acid desaturases, including ?5-desaturases, ?6-desaturases and ?12-desaturases, are used to generate transgenic plants, plant parts and cells which contain and express one or more transgenes encoding one or more desaturases. Expression of the desaturases with different substrate specificities in the plant system permit the large scale production of polyunsaturated long chain fatty acids such as docosahexaenoic acid, eicosapentaenoic acid, ?-linolenic acid, gamma-linolenic acid, arachidonic acid and the like for modification of the fatty acid profile of plants, plant parts and tissues. Manipulation of the fatty acid profiles allows for the production of commercial quantities of novel plant oils and products.Type: GrantFiled: May 31, 2006Date of Patent: February 16, 2010Assignee: Calgene LLCInventor: Debbie Knutzon