Abstract: A rapid method for generating a set of discrete DNA amplification products characteristic of a genome as a “fingerprint” comprises the steps of: priming target nucleic acid of a genome or from a cellular RNA preparation with an single-stranded primer to form primed nucleic acid such that a substantial degree of internal-mismatching occurs between the primer and the target nucleic acid; amplifying the primed nucleic acid by performing at least one cycle of polymerase chain reaction amplification; and amplifying the product of step (2) by performing at least about 10 cycles of polymerase chain reaction amplification. The method is known as the arbitrarily primed polymerase chain reaction (AP-PCR) method and is suitable for the identification of bacterial species and strains, mammals and plants.
Type:
Grant
Filed:
November 16, 1993
Date of Patent:
March 27, 2001
Assignee:
Stratagene and California Institute of Biological
Research
Abstract: A rapid method for generating a set of discrete DNA amplification products characteristic of a genome as a "fingerprint" comprises the steps of: priming target nucleic acid of a genome with an single-stranded primer to form primed nucleic acid such that a substantial degree of internal-mismatching occurs between the primer and the target nucleic acid; amplifying the primed nucleic acid by performing at least one cycle of polymerase chain reaction amplification; and amplifying the product of step (2) by performing at least about 10 cycles of polymerase chain reaction amplification. The method is known as the arbitrarily primed polymerase chain reaction (AP-PCR) method and is suitable for the identification of bacterial species and strains, including Staphylococcus and Streptococcus species, mammals and plants.
Type:
Grant
Filed:
June 6, 1995
Date of Patent:
January 19, 1999
Assignee:
Stratagene & California Institute Of Biological Research
Inventors:
Michael McClelland, John Thomas Welsh, Joseph A. Sorge
Abstract: A rapid method for generating a set of discrete DNA amplification products characteristic of a genome as a "fingerprint" for typing the genome comprises the steps of: forming a polymerase chain reaction (PCR) admixture by combining, in a PCR buffer, genomic DNA and at least one structural RNA consensus primer, and subjecting the PCR admixture to a plurality of PCR thermocycles to produce a plurality of DNA segments, thereby forming a set of discrete DNA amplification products. The method is known as the consensus sequence primed polymerase chain reaction (CP-PCR) method and is suitable for the identification of bacterial species and strains, including Staphylococcus and Streptococcus species, mammals and plants. The method of the present invention can identify species rapidly, using only a small amount of biological material, and does not require knowledge of the nucleotide sequence or other molecular biology of the nucleic acids of the organisms to be identified.
Type:
Grant
Filed:
February 25, 1991
Date of Patent:
August 1, 1995
Assignee:
California Institute of Biological Research
Abstract: Synthetic polypeptides corresponding to the B lymphocyte CR2 receptor binding site present on a CR2 ligand are disclosed together with polypeptide aggregates, compositions, anti-polypeptide antibodies and methods of preparing and using the polypeptides and antibodies.
Type:
Grant
Filed:
September 8, 1989
Date of Patent:
July 19, 1994
Assignee:
California Institute of Biological Research
Abstract: Synthetic polypeptides corresponding to the B lymphocyte CR2 receptor binding site present on an interferon alpha-related CR2 ligand are disclosed together with polypeptide aggregates, compositions, anti-polypeptide antibodies and methods of preparing and using the polypeptides and antibodies.
Type:
Grant
Filed:
April 20, 1990
Date of Patent:
May 10, 1994
Assignee:
California Institute of Biological Research
Abstract: Hybrid proteins containing repressor proteins and substituted receptor binding sites, amino acid and DNA sequences encoding the hybrid proteins are provided. Methods for preparing the hybrid proteins are also described.
Type:
Grant
Filed:
June 21, 1991
Date of Patent:
March 2, 1993
Assignee:
California Institute of Biological Research
Inventors:
Waldemar Lernhardt, Mario Bourdon, Phil Youderian