Patents Assigned to Cellectricon AB
-
Publication number: 20130052737Abstract: Disclosed is a method for selective electrofusion of at least two fusion partners having cell-like membranes and cellular or subcellular dimensions, comprising the following steps: A) the fusion partners are brought into contact with each other and B) an electrical field of a strength sufficient to obtain fusion and highly focused on the fusion partners is applied. The fusion partners are independently selected from the group consisting of a single cell, a liposome, a proteoliposome, a synthetic vesicle, an egg cell, an enucleated egg cell, a sperm cell at any development stage and a plant protoplast.Type: ApplicationFiled: July 23, 2012Publication date: February 28, 2013Applicant: CELLECTRICON ABInventors: Peter Eriksson, Daniel T. Chiu, Alexander Moscho, Owe Orwar, Richard N. Zare
-
Patent number: 8338150Abstract: Disclosed is a method for parallel delivery of agents to and/or into a cell structure, wherein at least two electrolyte-filled tubes are provided together with a counter electrode, the tubes being connected to a voltage or current generator, said agents being introduced into the electrolyte solution contained in the tubes, which are placed close to the cell structure, whereupon the agents are transported through the tubes to said cell structure and into the said structure through pores which have been formed by application of an electric field focused on the cell structure, resulting in electroporation of the cell structure. Also different applications of the method is disclosed, e.g. use of the method in order to transfer cell-impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure.Type: GrantFiled: June 9, 2008Date of Patent: December 25, 2012Assignee: Cellectricon ABInventors: Owe Orwar, Mattias Karlsson, Kerstein Nolkrantz, Cecilia Farre
-
Patent number: 8268555Abstract: Disclosed is a method for sequential delivery of agents to and/or into a cell structure, wherein an electrolyte-filled tube is provided together with a counter electrode, said tube is connected to a voltage or current generator, at least two agents are introduced in a discrete mode into the electrolyte solution contained in the tube, which is placed close to the cell structure, one agent at the time being transported through the tube to and/or into said cell structure in which a pore has been formed by application of an electric field focused on the cell structure, resulting in electroporation of the cell structure. Also different applications of the method is disclosed, e.g. us of the method in order to transfer cell-impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure.Type: GrantFiled: January 21, 2009Date of Patent: September 18, 2012Assignee: Cellectricon ABInventors: Owe Orwar, Mattias Karlsson, Cecilia Farre, Kerstin Nolkrantz
-
Patent number: 8232074Abstract: The present invention relates to methods of measuring electrical properties of a cell using electrode devices comprising tapered nanotips having submicrometer dimensions (“nanoelectrodes”) for insertion into a cell. The devices are used to measure electrical properties of the cell and, optionally, may be used to electroporate, the cell or subcellular structures within the cell. The invention also provides arrays of electrode devices having nanotips for simultaneously or sequentially measuring the electrical properties of cells (e.g., such as surface immobilized cells). The electrodes can be used to measure properties of ion channels and in HTS assays to identify drugs which affect the properties of ion channels. The invention additionally provides microfluidic systems adapted for use with the electrode devices having nanotips. In combination with the electrodes, the microfluidic systems provide cell-based biosensors for monitoring cellular responses to conditions, such as exposure to candidate drugs.Type: GrantFiled: October 15, 2003Date of Patent: July 31, 2012Assignee: Cellectricon ABInventors: Kent Jardemark, Aldo Jesorka, Mattias Karlsson, Jessica Olofsson, Owe Orwar, Johan Pihl, Eskil Sahlin
-
Publication number: 20120040370Abstract: The invention provides microfluidic systems for altering the solution environment around a nanoscopic or microscopic object, such as a sensor, and methods for using the same. The invention also provides a system and methods for modulating, controlling, preparing, and studying receptors.Type: ApplicationFiled: October 25, 2006Publication date: February 16, 2012Applicant: CELLECTRICON ABInventors: Owe Orwar, Daniel Chiu, Mattias Karlsson, Johan Pihl, Jon Sinclair, Frederik Pettersson
-
Patent number: 8057754Abstract: Apparatuses and methods of aligning at least one tip of a tip manifold with a plurality of wells of a multiwell plate. The tip manifold includes a plate, at least one tip depending from the plate, a first tip alignment pin depending from the plate, and a second tip alignment pin depending from the plate. The second tip alignment pin opposes the first tip alignment pin. The multiwell plate includes a body defining a plurality of non-porous wells for holding biological material, a first alignment hole, and a second alignment hole. The second alignment hole opposes the first alignment hole.Type: GrantFiled: March 10, 2009Date of Patent: November 15, 2011Assignee: Cellectricon ABInventors: Johan Pihl, Mattias Karlsson
-
Publication number: 20110183407Abstract: Apparatuses and methods of aligning at least one tip of a tip manifold with a plurality of wells of a multiwell plate. The tip manifold includes a plate, at least one tip depending from the plate, a first tip alignment pin depending from the plate, and a second tip alignment pin depending from the plate. The second tip alignment pin opposes the first tip alignment pin. The multiwell plate includes a body defining a plurality of non-porous wells for holding biological material, a first alignment hole, and a second alignment hole. The second alignment hole opposes the first alignment hole.Type: ApplicationFiled: March 10, 2009Publication date: July 28, 2011Applicant: CELLECTRICON ABInventors: Johan Pihl, Mattias Karlsson
-
Publication number: 20100159439Abstract: Disclosed is a method for sequential delivery of agents to and/or into a cell structure, wherein an electrolyte-filled tube is provided together with a counter electrode, said tube is connected to a voltage or current generator, at least two agents are introduced in a discrete mode into the electrolyte solution contained in the tube, which is placed close to the cell structure, one agent at the time being transported through the tube to and/or into said cell structure in which a pore has been formed by application of an electric field focused on the cell structure, resulting in electroporation of the cell structure. Also different applications of the method is disclosed, e.g. us of the method in order to transfer cell-impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure.Type: ApplicationFiled: January 21, 2009Publication date: June 24, 2010Applicant: Cellectricon ABInventors: Owe Orwar, Mattias Karlsson, Cecilia Farre, Kerstin Nolkrantz
-
Publication number: 20100143944Abstract: The invention provides microfluidic systems for altering the solution environment around a nanoscopic or microscopic object, such as a sensor, and methods for using the same. The invention can be applied in any sensor technology in which the sensing element needs to be exposed rapidly, sequentially, and controllably, to a large number of different solution environments whose characteristics may be known or unknown.Type: ApplicationFiled: February 25, 2009Publication date: June 10, 2010Applicant: Cellectricon ABInventors: Owe Orwar, Daniel Chiu, Johan Pihl, Jon Sinclair, Jessica Olofsson, Mattias Karlsson, Kent Jardemark
-
Publication number: 20090286297Abstract: Apparatuses and methods of aligning at least one tip of a tip manifold with a plurality of wells of a multiwell plate. The tip manifold includes a plate, at least one tip depending from the plate, a first tip alignment pin depending from the plate, and a second tip alignment pin depending from the plate. The second tip alignment pin opposes the first tip alignment pin. The multiwell plate includes a body defining a plurality of non-porous wells for holding biological material, a first alignment hole, and a second alignment hole. The second alignment hole opposes the first alignment hole.Type: ApplicationFiled: March 10, 2009Publication date: November 19, 2009Applicant: CELLECTRICON ABInventors: Johan Pihl, Mattias Karlsson
-
Patent number: 7563614Abstract: The invention provides microfluidic systems for altering the solution environment around a nanoscopic or microscopic object, such as a sensor, and methods for using the same. The invention can be applied in any sensor technology in which the sensing element needs to be exposed rapidly, sequentially, and controllably, to a large number of different solution environments whose characteristics may be known or unknown.Type: GrantFiled: January 15, 2003Date of Patent: July 21, 2009Assignee: Cellectricon ABInventors: Owe Orwar, Daniel Chiu, Johan Pihl, Jon Sinclair, Jessica Olofsson, Mattias Karlsson, Kent Jardemark
-
Publication number: 20090092963Abstract: Disclosed is a method for parallel delivery of agents to and/or into a cell structure, wherein at least two electrolyte-filled tubes are provided together with a counter electrode, the tubes being connected to a voltage or current generator, said agents being introduced into the electrolyte solution contained in the tubes, which are placed close to the cell structure, whereupon the agents are transported through the tubes to said cell structure and into the said structure through pores which have been formed by application of an electric field focused on the cell structure, resulting in electroporation of the cell structure. Also different applications of the method is disclosed, e.g. use of the method in order to transfer cell-impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure.Type: ApplicationFiled: June 9, 2008Publication date: April 9, 2009Applicant: Cellectricon ABInventors: Owe Orwar, Mattias Karlsson, Kerstein Nolkrantz, Cecilia Farre
-
Publication number: 20090047676Abstract: The invention provides a system, system components, and a method for rapidly obtaining and stably maintaining a cell in optimal contact with the cell-contacting surface of a sensor in a cell-based biosensor. In one aspect, the system maximizes the seal between a whole cell and the cell-contact surface of a patch clamp micropipette, maximizing the efficiency of a whole cell patch clamp recording.Type: ApplicationFiled: June 6, 2008Publication date: February 19, 2009Applicant: Cellectricon ABInventors: Mattias Karlsson, Owe Orwar, Daniel T. Chiu, Jon Sinclair, Kent Jardemark, Jessica Olofsson, Johan Pihl, Cecilia Farre
-
Patent number: 7470518Abstract: The invention provides microfluidic systems for altering the solution environment around a nanoscopic or microscopic object, such as a sensor, and methods for using the same. The invention also provides a system and methods for modulating, controlling, preparing, and studying receptors.Type: GrantFiled: January 6, 2005Date of Patent: December 30, 2008Assignee: Cellectricon ABInventors: Daniel Chiu, Owe Orwar, Kent Jardemark, Mattias Karlsson, Jessica Olofsson, Johan Pihl, Jon Sinclair
-
Patent number: 7456012Abstract: The invention provides hollow-tip-electrodes for spatially localized delivery of substances to one or more biological targets present in a population comprising target and non-target molecules, macromolecules, and/or cells. The invention also provides electrode plates for receiving one or more of such tips, tip-electrode plates comprising electrode plates comprising one or more electrode tips, and systems comprising tip-electrodes and containers for containing one or more biological targets, e.g., such as molecules, macromolecules, and/or cells. The invention further provides methods for using such systems and components thereof. In one preferred aspect, the systems are used for spatially confined electroporation of cells and cell structures. The invention facilitates high throughput screening of agents (e.g., such as drugs) that act on intracellular targets.Type: GrantFiled: December 2, 2003Date of Patent: November 25, 2008Assignee: Cellectricon ABInventors: Frida Ryttsén, Owe Orwar, Mikael Levin, Eskil Sahlin, Joakim Wigström
-
Patent number: 7393680Abstract: Disclosed is a method for penetration of lipid bilayer membranes in order to insert at the tip of a hollow needle-shaped object, such as a micropipet-, into a container formed of a lipid bilayer membrane, wherein said container is placed between said needle-shaped object, with the tip of said needle-shaped object placed in contact with said conainer in such a way that it applies a mechanical force to the lipid membrane of said container, thus mechanically straining it, and a second electrode, whereupon a transient electric pulse of 1-to-103 V/cm is applied between the electrodes, resulting in a focused electrical field over said container C which induces a dielectric breakdown of the lipid bilayer causing the needle-shaped object to penetrate the container. Disclosed is also an electroinjection method based on the above method, wherein substances are introduced through the needle-shaped object and into the container after penetration of the needle-shaped object.Type: GrantFiled: October 19, 2001Date of Patent: July 1, 2008Assignee: Cellectricon ABInventors: Owe Orwar, Mattias Karlsson, Daniel Chiu
-
Patent number: 7390650Abstract: The invention provides a system, system components, and a method for rapidly obtaining and stably maintaining a cell in optimal contact with the cell-contacting surface of a sensor in a cell-based biosensor. In one aspect, the system maximizes the seal between a whole cell and the cell-contact surface of a patch clamp micropipette, maximizing the efficiency of a whole cell patch clamp recording.Type: GrantFiled: August 20, 2003Date of Patent: June 24, 2008Assignee: Cellectricon ABInventors: Mattias Karlsson, Owe Orwar, Daniel T. Chiu, Jon Sinclair, Kent Jardemark, Jessica Olofsson, Johan Pihl, Cecilia Farre
-
Method for electro-permeabilization of individual cellular and organellar structures and use thereof
Patent number: 7109034Abstract: The invention relates to a method for permeabilization of a cell structure consisting of a single cell, an intracellular structure or an organelle comprising the following steps: (a) microelectrodes, preferably two carbon fiber electrodes or hollow fiber electrodes, are provided, (b) the microelectrodes are connected to a power supply, (c) the electrodes, individually controlled by high-graduation micromanipulators, are placed close to the cell structure at an appropriate inter-electrode distance, and (d) a highly focused electric field of a strength sufficient to obtain electroporation is applied between the electrodes. The method may be used in order to transfer cell impermeant solutes, such as drugs or genes, into the cell structure or out of the cell structure, in biosensors, in the treatment of tumours and neurodegenerative diseases and in the study of biophysical processes.Type: GrantFiled: December 19, 2002Date of Patent: September 19, 2006Assignee: Cellectricon ABInventors: Owe Orwar, Anders Lundqvist, Peter Eriksson, Daniel Chiu -
Patent number: 7018819Abstract: An apparatus and method are disclosed for electromanipulation of at least one cell or cell-like structure having cell-like membranes, the method comprising the steps: (a) at least one cell or cell-like structure is transported from one or more sample containers located on a chip through microchannel(s) located on said chip into a chamber located on said chip, wherein said chamber contains electrode(s) connected to a voltage generator, wherein said microchannel provides a fluid contact between the sample containers, (b) said cell or cell-like structure(s) is placed close to said at least one electrode, and (c) an electrical field is applied and focused on said cell or cell-like structure(s), said electrical field being of a strength sufficient to obtain pore-formation or fusion of said at least one cell or cell-like structure with another cell or cell-like structure(s) present in said chamber.Type: GrantFiled: November 30, 2001Date of Patent: March 28, 2006Assignee: Cellectricon ABInventors: Owe Orwar, Mattias Karlsson, Daniel Chiu, Anette Stromberg, Anders Karlsson
-
Patent number: D598128Type: GrantFiled: March 12, 2008Date of Patent: August 11, 2009Assignee: Cellectricon ABInventors: Johan Pihl, Mattias Karlsson