Patents Assigned to CELLFE, INC.
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METHODS AND SYSTEMS FOR MECHANOPORATION-BASED HIGH-THROUGHPUT PAYLOAD DELIVERY INTO BIOLOGICAL CELLS
Publication number: 20230416781Abstract: Described herein are methods and systems for mechanoporation-based high-throughput payload delivery into biological cells. For example, one system can process at least 1 billion cells per minute or at least 25 billion cells per minute, which is substantially greater than conventional methods. A cell processing apparatus comprises a processing assembly formed by stacking multiple processing components. Each processing component comprises channels, which may be used for filtration, mechanoporation, and/or separation of cells in the cell media. This functionality depends on the configuration of each channel. For example, each channel comprises one or more ridges such that each ridge forms a processing gap with an adjacent one of the processing components. The ridges may extend to the side walls or form a bypass gap with the wall. The processing gaps can be specially configured to compress cells as the cells pass through these gaps thereby initiating the mechanoporation process.Type: ApplicationFiled: August 25, 2023Publication date: December 28, 2023Applicant: CellFe, Inc.Inventors: Sewoon HAN, Ian SICHER, Alexander ALEXEEV, Ockchul KIM -
Methods and systems for mechanoporation-based high-throughput payload delivery into biological cells
Patent number: 11788050Abstract: Described herein are methods and systems for mechanoporation-based high-throughput payload delivery into biological cells. For example, one system can process at least 1 billion cells per minute or at least 25 billion cells per minute, which is substantially greater than conventional methods. A cell processing apparatus comprises a processing assembly formed by stacking multiple processing components. Each processing component comprises channels, which may be used for filtration, mechanoporation, and/or separation of cells in the cell media. This functionality depends on the configuration of each channel. For example, each channel comprises one or more ridges such that each ridge forms a processing gap with an adjacent one of the processing components. The ridges may extend to the side walls or form a bypass gap with the wall. The processing gaps can be specially configured to compress cells as the cells pass through these gaps thereby initiating the mechanoporation process.Type: GrantFiled: January 31, 2023Date of Patent: October 17, 2023Assignee: CellFe, Inc.Inventors: Sewoon Han, Ian Sicher, Alexander Alexeev, Ockchul Kim -
METHODS AND SYSTEMS FOR MECHANOPORATION-BASED HIGH-THROUGHPUT PAYLOAD DELIVERY INTO BIOLOGICAL CELLS
Publication number: 20230174918Abstract: Described herein are methods and systems for mechanoporation-based high-throughput payload delivery into biological cells. For example, one system can process at least 1 billion cells per minute or at least 25 billion cells per minute, which is substantially greater than conventional methods. A cell processing apparatus comprises a processing assembly formed by stacking multiple processing components. Each processing component comprises channels, which may be used for filtration, mechanoporation, and/or separation of cells in the cell media. This functionality depends on the configuration of each channel. For example, each channel comprises one or more ridges such that each ridge forms a processing gap with an adjacent one of the processing components. The ridges may extend to the side walls or form a bypass gap with the wall. The processing gaps can be specially configured to compress cells as the cells pass through these gaps thereby initiating the mechanoporation process.Type: ApplicationFiled: January 31, 2023Publication date: June 8, 2023Applicant: CellFe, Inc.Inventors: Sewoon HAN, Ian SICHER, Alexander ALEXEEV, Ockchul KIM -
Publication number: 20220298461Abstract: The present disclosure provides methods and systems for cell processing, including delivery of imaging agents into cells. The methods and systems may comprise the use of a microfluidic device. The microfluidic device may comprise a channel comprising a compressive element. The compressive element may be configured to reduce a volume of the cell and facilitate the formation of one or more transient pores in a cell membrane of the cell. The one or more pores may permit one or more imaging agents to enter the cell. Also provided are modified cells produced using the disclosed methods and systems and methods of imaging the modified cells in a subject.Type: ApplicationFiled: June 11, 2020Publication date: September 22, 2022Applicant: CELLFE, INC.Inventors: Alla ZAMARAYEVA, Alexander ALEXEEV, Todd SULCHEK, Sewoon HAN, Miguel CALERO-GARCIA, Ian SICHER
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Publication number: 20220213422Abstract: The present disclosure provides methods and systems for cell processing, including delivery of substances into cells. The methods and systems may comprise the use of a microfluidic device. The microfluidic device may comprise a channel comprising a compressive element. The compressive element may be configured to reduce a volume of the cell and facilitate the formation of one or more transient pores in a cell membrane of the cell. The one or more pores may permit one or more substances such as therapeutic or gene-editing reagents to enter the cell. Also provided are modified cells produced using the disclosed methods and systems.Type: ApplicationFiled: May 15, 2020Publication date: July 7, 2022Applicant: CELLFE, INC.Inventors: Alla ZAMARAYEVA, Alexander ALEXEEV, Todd SULCHEK, Miguel CALERO-GARCIA, Ian SICHER, Jocelyn LOO, Sewoon HAN
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Publication number: 20220056393Abstract: The present disclosure provides systems and methods for intracellular delivery. The systems and methods may comprise the use of a cell processing apparatus which may comprise a plurality of compression elements such as ridges. The intracellular delivery may be caused by rapid compression of cells, which may result in a reduction of a cell volume. The compression may occur while the cells pass through gaps formed by at least a subset of the ridges. The cell processing apparatus may further comprise one or more recovery spaces which are positioned between adjacent ridges. The cells may recover at least a portion of the reduced cell volume by absorbing media and/or reagents surrounding the cells while flowing through the recovery spaces. The ridges may also divert less compressible cells into a diversion channel, thereby sorting the cells based on various cell properties and/or preventing clogging within the apparatus.Type: ApplicationFiled: November 8, 2021Publication date: February 24, 2022Applicant: CELLFE, INC.Inventors: Sewoon HAN, Alexander ALEXEEV, Alla ZAMARAYEVA, Todd SULCHEK
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Publication number: 20210292700Abstract: The present disclosure provides systems and methods for intracellular delivery. The systems and methods may comprise the use of a cell processing apparatus which may comprise a plurality of compression elements such as ridges. The intracellular delivery may be caused by rapid compression of cells, which may result in a reduction of a cell volume. The compression may occur while the cells pass through gaps formed by at least a subset of the ridges. The cell processing apparatus may further comprise one or more recovery spaces which are positioned between adjacent ridges. The cells may recover at least a portion of the reduced cell volume by absorbing media and/or reagents surrounding the cells while flowing through the recovery spaces. The ridges may also divert less compressible cells into a diversion channel, thereby sorting the cells based on various cell properties and/or preventing clogging within the apparatus.Type: ApplicationFiled: June 4, 2021Publication date: September 23, 2021Applicant: CELLFE, INC.Inventors: Sewoon HAN, Alexander ALEXEEV, Alla ZAMARAYEVA, Todd SULCHEK