Abstract: An apparatus for microfluidic flow cytometry analysis of a particulate containing fluid An apparatus for microfluidic flow cytometry analysis of a particulate containing fluid comprises a hydrodynamic focussing apparatus for providing a focused stream of particulate containing fluid; and a microfluidic chip. The chip has a plurality of layers and comprises a microfluidic channel that extends through the chip substantially orthogonal to a plane of the layers of the chip, and is in fluid communication with the hydrodynamic focusing apparatus for receipt of a focused steam of particulate containing fluid. The chip also comprises a detection zone comprising at least one pair of electrodes in electrical communication with the microfluidic channel.

Abstract: This invention relates to the field of microfluidic flow cytometry and more generally microfluidic techniques for analysis of particulate-containing fluids. It deals with the improvements to such technologies in order to identify subsets of particles or sub-populations of cells that differ in their properties, and, if necessary, separate the said identified sub-populations of cells, e.g. sex of semen cells, alive cells from the dead ones, cancerous cells from the healthy ones, subsets of viruses, bacteria or subsets of particles. This invention disclosure deals with the apparatus and the method for detection of cells or particles based on measurements of complex AC impedance between electrodes across the flow of fluid containing such cells or particles.

Abstract: A microfluidic chip for focussing a stream of particulate containing fluid comprises a sample microfluidic channel configured to receive the stream of particulate containing fluid, a guidance microfluidic channel having a polygonal cross-sectional area and configured to receive a stream of guidance fluid, and a common microfluidic channel having a polygonal cross sectional area formed by the merging of the sample microfluidic channel and the guidance 10 microfluidic channel at an oblique angle along only part of one or more sides of the guidance microfluidic channel, and a detection zone disposed in the common microfluidic channel having one or more sensors.

Abstract: A system for continuous high-throughput treatment, in particular electroporation or transfection, of a population of cells or selected cells in a population of cells, is described. The system comprises a fluidic device comprising a microfluidic channel, a pump fluidically coupled to the fluidic device and configured to pump the population of cells in a carrier liquid unidirectionally along the microfluidic channel, and a processor. The microfluidic channel has an upstream detection zone comprising a detection electrode module configured to detect a change in electrical impedance across the microfluidic channel at the upstream detection zone corresponding to a cell passing the upstream detection zone and a cell treatment zone located downstream of the upstream detection zone and comprising a cell treatment module configured to treat the cell passing the cell treatment zone.

Abstract: An apparatus for microfluidic flow cytometry analysis of a particulate containing fluid An apparatus for microfluidic flow cytometry analysis of a particulate containing fluid comprises a hydrodynamic focussing apparatus for providing a focused stream of particulate containing fluid; and a microfluidic chip. The chip has a plurality of layers and comprises a microfluidic channel that extends through the chip substantially orthogonal to a plane of the layers of the chip, and is in fluid communication with the hydrodynamic focusing apparatus for receipt of a focused steam of particulate containing fluid. The chip also comprises a detection zone comprising at least one pair of electrodes in electrical communication with the microfluidic channel.

Abstract: The present invention is directed to a method for measuring the migration of cells in a channel under the influence of an analyte wherein said cells are separated from said analyte by a semi-permeable membrane and said cells are subjected to controlled flow conditions while the analyte is static and in the form of a viscous substance or gel.

Abstract: An apparatus (1) for culturing live cells comprising a cell culture vessel (5), a fresh culture medium storage vessel (3), a used culture medium collection vessel (4), a supply conduit (9, 10) adapted to provide fluid communication between the fresh culture medium storage vessel and a first end of the cell culture vessel, an inlet of the supply conduit being located towards a base of the fresh culture medium storage vessel, a drainage conduit (12) adapted to provide fluid communication between a second end of the cell culture vessel and the used culture medium collection vessel, an inlet of the drainage conduit being disposed within the cell culture vessel such that in use it is it located in the culture medium, and a pump (2) adapted to pump fresh culture medium from the fresh culture medium storage vessel (3) to one end of the cell culture vessel (5) and pump used culture medium from the second end of the cell culture vessel to the used culture medium collection vessel (4).

Abstract: The present invention is directed to a method for measuring the migration of cells in a channel under the influence of an analyte wherein said cells are separated from said analyte by a semi-permeable membrane and said cells are subjected to controlled flow conditions while the analyte is static and in the form of a viscous substance or gel.