Abstract: Azide-free blood diluents and cyanide ion-free erythrocyte lysing reagents hich are particularly advantageous for use with Coulter scanning devices. The lysing reagent comprises (1) a water-soluble quaternary ammonium salt of the formula R.sub.1 N(R.sub.2).sub.3 X wherein R.sub.1 is a 12-20 C alkyl, each R.sub.2 is the same or different and is lower alkyl or lower hydroxyalkyl and X is an anion, and (2) a minor amount of a water-soluble polycarboxylic acid.
Type:
Grant
Filed:
February 8, 1977
Date of Patent:
January 29, 1980
Assignee:
Central Laboratories of Associated Maryland Pathologists, Ltd.