Patents Assigned to Cepheid
  • Methods for DNA and RNA extraction from fixed paraffin-embedded tissue samples
    Patent number: 10233440
    Abstract: Methods and reagents are provided for the rapid extraction of nucleic acids from a fixed paraffin embedded sample (e.g., a FFPET sample). In some embodiments, the methods comprise incubating one or more sections of said tissue sample in a lysis solution comprising a buffer sufficient to maintain the pH of said solution at a pH ranging from about pH 4 to about pH 9; a chaotropic agent; a chelating agent; and a detergent; where the incubating is at a temperature ranging from about 50 C to about 100 C; and recovering the nucleic acid from said lysis solution.
    Type: Grant
    Filed: September 26, 2013
    Date of Patent: March 19, 2019
    Assignee: CEPHEID
    Inventors: Edwin Wei-Lung Lai, Reuel Van Atta, Kenneth E. Ho
  • Compositions and methods for detecting markers associated with bladder cancer
    Patent number: 10202653
    Abstract: Compositions and methods for detecting bladder cancer are provided. In some embodiments, methods of monitoring recurrence of bladder cancer are provided. In some embodiments, the methods comprise detecting a set of markers consisting of CRH, IGF2, KRT20, and ANXA10.
    Type: Grant
    Filed: April 19, 2013
    Date of Patent: February 12, 2019
    Assignee: Cepheid
    Inventors: Russell Higuchi, Stacey Ellen Wallace, Edwin Wei-Lung Lai
  • Honeycomb tube
    Patent number: 10190165
    Abstract: A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate.
    Type: Grant
    Filed: September 26, 2013
    Date of Patent: January 29, 2019
    Assignee: Cepheid
    Inventors: Yuh-Min Chiang, Doug Dority, Dustin Dickens, Jennifer Glass, Reuel Van Atta
  • Apparatus with heterogeneous processing modules
    Patent number: 10132728
    Abstract: A biological sample processing apparatus having an enclosure. A plurality of sample processing modules are held by the enclosure. Each sample processing module is configured to hold a removable sample cartridge and to only perform sample processing on a sample within the corresponding removable sample cartridge. Each sample processing module is configured to perform at least one of a plurality of testing processes on the sample within the removable sample cartridge. At least one module in the apparatus is configured to perform nucleic acid amplification and detection.
    Type: Grant
    Filed: April 25, 2013
    Date of Patent: November 20, 2018
    Assignee: Cepheid
    Inventor: John L. Bishop
  • Methods of detecting cervical cancer
    Patent number: 10000808
    Abstract: Methods of detecting cervical dysplasia, such as cervical dysplasia likely to progress to carcinoma in a sample of human cervical cells, are provided. Methods of detecting changes in expression of one or more microRNAs or mRNAs associated with cervical dysplasia or cervical cancer are also provided. Compositions and kits are also provided.
    Type: Grant
    Filed: July 29, 2014
    Date of Patent: June 19, 2018
    Assignee: Cepheid
    Inventors: Cecilia Svanholm Barrie, Olivier Delfour, David H. Persing
  • Apparatus and method for cell disruption
    Patent number: 9943848
    Abstract: An apparatus for disrupting cells or viruses comprises a container having a chamber for holding the cells or viruses. The container includes at least one flexible wall defining the chamber. The apparatus also includes a transducer for impacting an external surface of the flexible wall to generate pressure waves in the chamber. The apparatus also includes a pressure source for increasing the pressure in the chamber. The pressurization of the chamber ensures effective coupling between the transducer and the flexible wall. The apparatus may also include beads in the chamber for rupturing the cells or viruses.
    Type: Grant
    Filed: June 12, 2015
    Date of Patent: April 17, 2018
    Assignee: Cepheid
    Inventors: Michael T. Taylor, Phillip Belgrader, Farzad Pourahmadi, William A. McMillan, Ronald Chang, Stanley H. Sakai, Jesus Ching
  • Honeycomb tube
    Patent number: 9914968
    Abstract: A honeycomb tube with a planar frame defining a fluidic path between a first planar surface and a second planar surface. A fluidic interface is located at one end of the planar frame. The fluidic interface has a fluidic inlet and fluidic outlet. The fluidic path further includes a well chamber having an well-substrate with a plurality of wells. The well chamber is arranged in the planar frame between the first or second surface and the well-substrate. The well chamber is in fluidic communication between the pre-amplification chamber and the fluidic outlet.
    Type: Grant
    Filed: March 15, 2013
    Date of Patent: March 13, 2018
    Assignee: Cepheid
    Inventors: Yuh-Min Chiang, Doug Dority, Dustin Dickens, Jennifer Glass, Reuel Van Atta
  • Thermal cycling apparatus and method
    Patent number: 9908119
    Abstract: A thermal cycling apparatus having a sample interfacing wall extending from a mounting wall. The sample interfacing wall can accept and apply thermal cycles to samples. An air source can direct an air stream to cool the sample. Another source can direct heated air away from the sample.
    Type: Grant
    Filed: May 15, 2013
    Date of Patent: March 6, 2018
    Assignee: Cepheid
    Inventor: Doug Dority
  • Methods and apparatus for sequential amplification reactions
    Patent number: 9873909
    Abstract: The invention provides methods and apparatus for carrying out multiple amplification reactions in a single reaction chamber by successive cycles of loading reaction mixture, amplifying, and removing spent reaction mixture in a fluidly closed reaction system. In particular, the present invention allows amplification of a plurality of target polynucleotides from a single sample by carrying out under closed-loop control successive amplifications of different target polynucleotides from different portions of the sample.
    Type: Grant
    Filed: November 25, 2014
    Date of Patent: January 23, 2018
    Assignee: Cepheid
    Inventors: Joseph H. Smith, David H. Persing, Alan Wortman, Ronald Chang, David Swenson
  • Device for extracting nucleic acid from a sample
    Patent number: 9789481
    Abstract: The present invention provides a cartridge for analyzing a fluid sample. The cartridge provides for the efficient separation of cells or viruses in the sample from the remaining sample fluid, lysis of the cells or viruses to release the analyte (e.g., nucleic acid) therefrom, and optionally chemical reaction and/or detection of the analyte. The cartridge is useful in a variety of diagnostic, life science research, environmental, or forensic applications for determining the presence or absence of one or more analytes in a sample.
    Type: Grant
    Filed: October 24, 2007
    Date of Patent: October 17, 2017
    Assignee: Cepheid
    Inventors: Kurt E. Petersen, Michael T. Taylor, Farzad Pourahmadi, William A. McMillan, Ronald Chang, Stanley H. Sakai, Jesus Ching, Douglas B. Dority, Phillip Belgrader, M. Allen Northrup
  • METHODS OF DETECTING INFLUENZA
    Publication number: 20170275712
    Abstract: Compositions and methods for detecting influenza are provided.
    Type: Application
    Filed: August 22, 2014
    Publication date: September 28, 2017
    Applicant: CEPHEID
    Inventors: Anupama MOKKAPATI, Bradley BROWN, Robert JONES
  • METHODS OF DETECTING TRICHOMONAS VAGINALIS
    Publication number: 20170183746
    Abstract: Compositions and methods for detecting Trichomonas vaginalis are provided.
    Type: Application
    Filed: July 15, 2014
    Publication date: June 29, 2017
    Applicant: Cepheid
    Inventors: James Wang, Sudhir Alugupally, Rosa Yu, Sally Yousif
  • Fluid processing and control
    Patent number: 9669409
    Abstract: A fluid control and processing system for controlling fluid flow among a plurality of chambers comprises a body including a fluid processing region continuously coupled fluidicly with a fluid displacement region. The fluid displacement region is depressurizable to draw fluid into the fluid displacement region and pressurizable to expel fluid from the fluid displacement region. The body includes at least one external port. The fluid processing region is fluidicly coupled with the at least one external port. The fluid displacement region is fluidicly coupled with at least one external port of the body. The body is adjustable with respect to the plurality of chambers to place the at least one external port selectively in fluidic communication with the plurality of chambers.
    Type: Grant
    Filed: November 6, 2015
    Date of Patent: June 6, 2017
    Assignee: Cepheid
    Inventors: Douglas B. Dority, Ronald Chang
  • DNA recombination junction detection
    Patent number: 9605322
    Abstract: The present invention provides methods, compositions and kits for detecting the presence or absence of an integrated insertion polynucleotide.
    Type: Grant
    Filed: January 7, 2014
    Date of Patent: March 28, 2017
    Assignee: Cepheid
    Inventors: Jian Ping Zhang, Ernest Jay Friedlander, Robert Ruhfel, David Douglas Swenson, Alan Thomas Wortman
  • Modified thymine polynucleotide oligomers and methods
    Patent number: 9598455
    Abstract: Disclosed are modified thymine bases that provide enhanced base-pairing affinity for adenine or 2,6-diaminopurine bases in polynucleotide hybridization complexes. Also disclosed are polynucleotide oligomers, polynucleotide hybridization complexes that comprise such modified thymine bases. Also disclosed are various methods of use. For example, in some embodiments, modified polynucleotide oligomers disclosed herein can be used as primers and probes for nucleic acid amplification and/or detection.
    Type: Grant
    Filed: March 30, 2015
    Date of Patent: March 21, 2017
    Assignee: Cepheid
    Inventors: Alexander A. Gall, Sergey G. Lokhov, Mikhail A. Podyminogin, Ekaterina V. Viazovkina, Kevin Patrick Lund
  • Modified cytosine polynucleotide oligomers and methods
    Patent number: 9598456
    Abstract: Disclosed are modified cytosine bases that provide enhanced base-pairing affinity for guanine bases in polynucleotide hybridization complexes. Also disclosed are polynucleotide oligomers, polynucleotide hybridization complexes that comprise such modified cytosine bases. Also disclosed are various methods of use. For example, in some embodiments, modified polynucleotide oligomers disclosed herein can be used as primers and probes for nucleic acid amplification and/or detection.
    Type: Grant
    Filed: March 30, 2015
    Date of Patent: March 21, 2017
    Assignee: Cepheid
    Inventors: Alexander A. Gall, Sergey G. Lokhov, Mikhail A. Podyminogin, Ekaterina V. Viazovkina, Kevin Patrick Lund
  • Methods of detecting sepsis
    Patent number: 9493832
    Abstract: Methods of detecting sepsis in a sample from a patient are provided. Methods of detecting changes in expression of one or more microRNAs associated with sepsis are also provided. Compositions and kits are also provided.
    Type: Grant
    Filed: February 2, 2010
    Date of Patent: November 15, 2016
    Assignee: Cepheid
    Inventors: David Vilanova, David H. Persing, Olivier Delfour, Bernard Michot
  • Molecular diagnostic system
    Patent number: D775363
    Type: Grant
    Filed: July 24, 2015
    Date of Patent: December 27, 2016
    Assignee: CEPHEID
    Inventors: Ronald Chang, Steven M. Montgomery, Gregory E. Mote
  • Molecular diagnostic system
    Patent number: D802786
    Type: Grant
    Filed: November 14, 2016
    Date of Patent: November 14, 2017
    Assignee: Cepheid
    Inventors: Ronald Chang, Steven M. Montgomery, Gregory E. Mote
  • Molecular diagnostic system
    Patent number: D804049
    Type: Grant
    Filed: November 14, 2016
    Date of Patent: November 28, 2017
    Assignee: Cepheid
    Inventors: Ronald Chang, Steven M. Montgomery, Gregory E. Mote