Abstract: The present invention provides a process for isolating vascular endothelial cells from embryoid bodies differentiated from embryonic stem cells, which comprises: (a) treating embryoid bodies differentiated from embryonic stem cells with 0.005-0.015% trypsin and 0.05-0.15 mM ethylenediaminetetraacetate (EDTA) to obtain vascular endothelial cell clusters; and (b) treating the vascular endothelial cell clusters with 0.1-0.5% trypsin and 0.5-2 mM EDTA so as to separate the vascular endothelial cell clusters into single cells.
Type:
Application
Filed:
June 13, 2007
Publication date:
June 10, 2010
Applicant:
CHABIOTECH CO., LTD.
Inventors:
Hyung-Min Chung, Sung-Hwan Moon, Ju-Mi Kim, Soo-Hong Lee
Abstract: The present invention relates to a method for culturing human embryonic stem cells (hESCs) in a hESC culture medium comprising a porous membrane, feeder cells being attached to a bottom of the porous membrane and a method for recovering human embryonic stem cells using the same.
Type:
Application
Filed:
May 31, 2007
Publication date:
May 21, 2009
Applicants:
CHABIOTECH CO., LTD., COLLEGE OF MEDICINE POCHON CHA UNIVERSITY INDUSTRY-ACADEMIC COOPERATION FOUNDATION
Inventors:
Hyung-Min Chung, Soo-Hong Lee, Si-Nae Kim, Min-Jeong Kim
Abstract: Provided are methods for the vitrification of human oocytes, which comprises: (a) placing human oocytes on a transfer instrument; and (b) placing the transfer instrument and the human oocytes directly into a slushed nitrogen (N2 slush), wherein the human oocytes are directly exposed to the N2 slush thereby undergoing vitrification, and wherein the human oocytes are able to live for a period of time after the human oocytes are devitrified.
Type:
Application
Filed:
April 17, 2006
Publication date:
March 26, 2009
Applicant:
CHABIOTECH CO., LTD.
Inventors:
Tae Ki Yoon, Dong Ryul Lee, Hyung Min Chung, Kwang Yul Cha
Abstract: Provided is a PHB block copolymer having orientation-induced rubber-elasticity and temperature-sensitive shape memory effects, comprising a plurality of 3-hydroxybutyrate (3HB) blocks as a repeating unit and a plurality of 3-hydroxyvalerate (3HV) blocks as a repeating unit, and optionally a hydroxy acid repeating group containing 6 or more carbon atoms. The PHA block copolymer exhibits orientation-induced rubber-elasticity and shape memory effects with a fast shape-recovery rate, and therefore such characteristics in conjunction with physical properties such as biodegradability and biocompatibility unique to PHA enable application thereof to a variety of uses.
Type:
Application
Filed:
December 22, 2005
Publication date:
October 2, 2008
Applicant:
CHABIOTECH CO., LTD
Inventors:
Young Ha Rhee, Young baek Kim, Chungwook Chung, Jooseog Yoon
Abstract: A children's shoe (1) for the stimulation of the plantar arches to the ends of the prevention or cure of flat foot, comprising a cushion-shaped element (6) having a sandwich-shaped construction, extending in correspondence of the internal portion (4) of the sole (3) of the shoe (1) and exhibiting a substantial elastic deformability during a subject's walking cycle.
Abstract: The present invention discloses methods for isolating, proliferating and differentiating germ-line stem cell in vitro. More specifically, the present invention discloses a method for the in vitro isolation and proliferation of mammalian germ-line stem cells, characterized in culturing cells isolated from mammalian testis in an embryonic stem cell culture medium, and for the in vitro differentiation, characterized in encapsulating mammalian germ-line stem cells and Sertoli cells with calcium alginate and co-culturing with peritubular cells. Also, the present invention discloses germ-line stem cells obtained by above methods, a composition for the treatment of male infertility, comprising the germ-line stem cells, and a method for the treatment of male infertility by the use of the germ-line stem cells.