Abstract: The present invention provides a process for efficiently producing sporangia of Bacillus popilliae containing spores and parasporal bodies having controlling effects on Scarabaeidae insects, and a control agent and controlling method for Scarabaeidae insects obtained by said production process. In a process for producing sporangia of Bacillus popilliae containing spores and parasporal bodies by culturing Bacillus popilliae in a medium containing an adsorbent, the medium contains 0.2-4.0% by weight of glutamic acid.
Type:
Grant
Filed:
December 9, 2003
Date of Patent:
January 15, 2008
Assignees:
Dainippon Ink and Chemicals, Inc., Chiba Prefectural Government
Abstract: The present invention provides a process for producing sporangia of Bacillus popilliae containing spores and parasporal bodies in large numbers per unit volume of medium. In a process for producing sporangia of Bacillus popilliae containing spores and parasporal bodies by culturing Bacillus popilliae in a liquid medium containing an adsorbent, the liquid medium contains 0.1–0.7% by weight of proline.
Type:
Grant
Filed:
December 23, 2003
Date of Patent:
August 8, 2006
Assignees:
Dainippon Ink and Chemicals, Inc., Chiba Prefectural Government
Abstract: The present invention discloses an attenuated smallpox vaccine strain exhibiting antibody production similar to conventional strains but without postvaccinal side effects. The vaccine is prepared by attenuating a Lister strain of a vaccinia virus by cell culture and selecting a suitable strain therefrom showing relatively small and uniform pocks on the chorioallantoic membrane of an embryonated egg.