Abstract: Surgical simulation devices and their methods of use are described. In some embodiments, a surgical simulation device includes a base, a first surgical model configured to simulate a first cleft lip condition, and a second surgical model configured to simulate a second cleft lip condition. The device includes a model support configured to be releasably engaged with the base. The first surgical model is disposed on a first surface of the model support and the second surgical model is disposed on a second surface of the model support. The base includes an internal volume sized and shaped to receive one of the first surgical model and the second surgical model. The other of the first surgical model and the second surgical model is at least partially exposed when the model support is engaged with the base.

Abstract: Described herein are methods and compositions for treating cancer. Aspects of the invention relate to administering to a subject having cancer an asparaginase and an agent that inhibits G6PD. Another aspect of the invention relates to administering an asparaginase to a subject having cancer that comprises a G6PD deficiency.

Abstract: Provided are methods and compositions for treating cerebrospinal fluid disorders. In embodiments, the methods comprise administering an agent to a subject altering activity, expression, or level of NKCC1 in a cell.

Abstract: Described herein are engineered ligand that binds a cell surface receptor (e.g., GPCR), with improved affinity, potency, and specificity. By conjugating a sub-optimal ligand for a cell surface receptor (e.g., GPCR) to a targeting molecule that binds an epitope (natural or exogenous epitope) in the extracellular portion of the cell surface receptor (e.g., GPCR), the affinity, potency, and/or specificity of the sub-optimal ligand is enhanced.

Abstract: Described herein are methods and compositions for producing modified, PD-L1 expressing hematopoietic stem cells, and uses thereof. Aspects of the invention relate to modulating the expression of micro RNA that controls the expression of PD-L1 in the hematopoietic stem cell. Methods for modulating the expression of micro RNA include, e.g., introducing to the cell a nucleic acid encoding a given micro RNA, or an agent that inhibits a given micro RNA.

Abstract: The invention features a prospective respiratory motion compensation technique for cardiovascular magnetic resonance imaging of the whole-heart of a free-breathing subject.

Abstract: Provided herein are products and methods for detecting analytes using polymers that bind to such analytes and thereby undergo a conformational change or contribute to a newly formed complex.

Abstract: Methods and systems for cardiovascular structure reconstruction are provided. A method of reconstructing a cardiovascular structure may include imaging the cardiovascular structure, producing a three-dimensional model of the cardiovascular structure based on the imaging, and creating a three-dimensional model of a patch corresponding to the three-dimensional model of the cardiovascular structure, where the patch is configured to reconstruct the cardiovascular structure to a normal geometry. A patch for a cardiovascular structure may include at least one layer of anisotropic material including an outermost perimeter and one or more notches extending inward from the outermost perimeter, where each of the one or more notches creates a discontinuity in the outermost perimeter. In some cases, each of the one or more notches includes a first edge and a second opposing edge, and the patch further includes one or more sutures configured to secure the first edge to the second opposing edge.

Abstract: The invention provides methods for activating a repressed allele within an imprinting control region, thereby treating an imprinting associated disorder.

Abstract: Disclosed herein are methods for generating mature cardiomyocytes and compositions including mature cardiomyocytes. Also disclosed herein are methods for enhancing maturation of quiescent cardiomyocytes and compositions including mature quiescent cardiomyocytes.

Abstract: This disclosure provides new and improved systems and methods for analyzing binding interactions and for identifying and measuring agents that modulate such binding interactions, including weak binding interactions. The methods may be used in high throughput screening assays.

Abstract: Systems and methods of the present disclosure enable improved seizure detection and/or prediction using a seizure monitoring system. The system receives a data stream including wearable sensor data associated with a user, where the data stream includes electrodermal activity data and where the electrodermal activity data includes circadian rhythm-dependent amplitudes. The system receives a time associated with a seizure of the user. The system trains seizure machine learning model to identify a pre-ictal period associated with a time segment based on the circadian rhythm dependent amplitudes and the time associated with the seizure. The system deploys the seizure machine learning model to ingest a new data stream. Based on the new data stream, the seizure machine learning model predicts a seizure likelihood in a prediction period.

Abstract: Provided herein are agents that inhibit the function (e.g., the ability to repress Tsp-1) of Protease, Serine 2 (PRSS2) by inhibiting the binding of PRSS2 to LRP1. Further provided herein are agents that bind to binding domain I of LRP1 and mimic the activity of prosaposin in stimulating Tsp-1 Methods of using these agents in treating cancer are also provided.

Abstract: Methods for preparing and using collagen extracts and collagen scaffolds are provided. Additionally methods and related kits for the repair of articular tissue using the collagen material are provided.

Abstract: Surgical simulation devices including surgical models of various anatomical malformations as well as their methods of use and manufacture are described. In some embodiments, a surgical simulation device includes a base, a surgical model that simulates an anorectal malformation, and a model support. The model support is an internal volume sized and shaped to receive at least a portion of the surgical model such that an operating surface of the surgical model is exposed. The model support is releasably engaged with the base. In other embodiments, surgical models with simulated tissues including tissue reinforcement are described.

Abstract: Surgical simulation devices including surgical models of various anatomical malformations as well as their methods of use and manufacture are described. In some embodiments, a surgical simulation device includes a base, a surgical model that simulates an anorectal malformation, and a model support. The model support is an internal volume sized and shaped to receive at least a portion of the surgical model such that an operating surface of the surgical model is exposed. The model support is releasably engaged with the base. In other embodiments, surgical models with simulated tissues including tissue reinforcement are described.

Abstract: Systems and methods of the present disclosure determine whether the patient experiences a grand tonic clonic seizures using video recordings or sensor data or both. The systems and methods receive data from a device that continuously records video and/or sensor data, continuously analyzes the data with a processing unit utilizing machine learning to classify segments of the data as seizure or no seizure, and to classify seizure types. An alarm is produced using an output unit when an epileptic data segment is detected. The processing unit thus provides continuous and in real-time monitoring of an epilepsy patient in the home or hospital setting, e.g. while the patient is sleeping in bed. In case a seizure is detected, an alarm may inform caregivers or clinicians to help them intervene and limit the complications of this seizure for the patient.

Abstract: Methods for preparing and using collagen extracts and collagen scaffolds are provided. Additionally, methods and related kits for the repair of articular tissue using the collagen material are provided.

Abstract: An engineering workflow for prospective design of heart valve leaflet grafts for surgical heart valve reconstruction is disclosed. The engineering workflow includes utilizing a quantitative description of one or more mechanical properties of one or more materials to be used for the heart valve reconstruction to prescribe a size and/or a shape of the materials to achieve a predetermined configuration of a final reconstructed heart valve in its physiological working state for a given patient.

Abstract: Provided herein are anti-Notch4 antibodies or antigen binding fragments thereof and their use in the treatment of a disease or disorder associated with increased levels of Notch4.