Abstract: A hyperspectral imaging method includes: providing time-domain synchronous mid-infrared ultrashort pulse and near-infrared ultrashort pulse as pump light and signal light, respectively; subjecting the signal light to optical time-stretching to broaden a pulse width of the signal light; directing the time-stretched signal light to a target sample to be detected; directing the pump light to a time delayer to adjust the time when the pump light reaches a silicon-based camera; spatially combining the time-stretched signal light from the target sample with the pump light from the time delayer; directing combined light to a silicon-based camera where the signal light is detected through non-degenerate two-photon absorption of the signal light under the action of the pump light to acquire hyperspectral imaging data; and obtaining an image of the target sample based on the hyperspectral imaging data.