Abstract: Detecting a definitive presence or absence of an analyte from a borderline test is shown and described. In one embodiment, a method of generating a definitive test result includes continuously incubating the assay concurrently with continuously reading a diagnostic test on the assay. In particular examples, reading the diagnostic test on the assay includes performing a one minute diagnostic reading. Typically, when a borderline test result is detected, further development of the assay is performed. In most examples, detecting a definitive presence or absence test result deactivates the testing sequence.