Abstract: The disclosure provides methods and compositions useful for labeling of target molecules with origin-specific nucleic acid identifiers (for example, barcodes), which can be used subsequently to identify, quantify, or otherwise characterize a feature or activity of target molecules originating from a particular discreet volume. Such target molecules can include polypeptides expressed by cells, in which nucleic acid molecules encoding the polypeptides are labeled with the same, or matched, origin-specific nucleic acid identifiers.
Type:
Grant
Filed:
March 11, 2016
Date of Patent:
January 16, 2024
Assignees:
The Broad Institute, Inc., ÉCOLE SUPERIEURE DE PHYSIQUE ET DE CHIMIE INDUSTRIELLES DE LA VILLE DE PARIS (ESPCI PARIS TECH)
Inventors:
Robert Nicol, Andrew David Griffiths, Baptiste Saudemont, Timothy V. Kirk