Patents Assigned to Colifast AS
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Patent number: 7811783Abstract: A rapid method for detecting spoilage of a food sample, particularly a fish sample, by detecting and enumerating sulfide-producing bacteria (SPB). A growth medium containing iron and sulfur is combined with the food sample forming an incubation mixture which is incubated for a period of time. In one embodiment, a plurality of fluorescence measurements are taken during an incubation period of about 3 hours to 17 hours at 30° C. SPB are determined to be present in the sample if the fluorescence measurement initially increases and then decreases to form a fluorescence maximum (peak). The time to detection of the fluorescence peak can be used with a correlation schedule to enumerate the SPB in the food sample. In another embodiment, a visual test can also be used to identify color changes in the incubation mixture to provide a semi-quantitative enumeration of SPB effective after about 3 hours to 17 hours of incubation.Type: GrantFiled: October 29, 2007Date of Patent: October 12, 2010Assignee: Colifast ASInventors: Grete Lorentzen, Olaug Taran Skjerdal, James D. Berg
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Patent number: 6908746Abstract: A rapid method for detecting spoilage of a food sample, particularly a fish sample, by detecting and enumerating sulfide-producing bacteria (SPB). A growth medium containing iron and sulfur is combined with the food sample forming an incubation mixture which is incubated for a period of time. In one embodiment, a plurality of fluorescence measurements are taken during an incubation period of about 3 hours to 17 hours at 30° C. SPB are determined to be present in the sample if the fluorescence measurement initially increases and then decreases to form a fluorescence maximum (peak). The time to detection of the fluorescence peak can be used with a correlation schedule to enumerate the SPB in the food sample. In another embodiment, a visual test can also be used to identify color changes in the incubation mixture to provide a semi-quantitative enumeration of SPB effective after about 3 hours to 17 hours of incubation.Type: GrantFiled: October 10, 2003Date of Patent: June 21, 2005Assignee: Colifast ASInventors: Grete Lorentzen, Olaug Taran Skjerdal, James D. Berg
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Patent number: 6723505Abstract: A method for the identification of the presence of E. coli, and Enterococcus faecalis and/or Enterococcus faecium in liquid or liquified samples. The method employs novel oligonucleotide primers based on sequences of the E. coli LamB gene and the Enterococcus faecalis/faecium transposase gene Tn1546. The method involves enrichment of the liquid or liquified sample, preferably using a selective medium, obtaining DNA from bacteria in the sample, and PCR amplification of the DNA using the novel oligonucleotide primers. Kits with the primers are also contemplated.Type: GrantFiled: August 8, 2000Date of Patent: April 20, 2004Assignee: Nye Colifast ASInventor: Frank Karlsen
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Patent number: 6632632Abstract: A rapid method for detecting spoilage of a food sample, particularly a fish sample, by detecting and enumerating sulfide-producing bacteria (SPB). A growth medium containing iron and sulfur is combined with the food sample forming an incubation mixture which is incubated for a period of time. A plurality of fluorescence measurements are taken during an incubation period of about 4 hours to 17 hours at 30° C. SPB are determined to be present in the sample if the fluorescence measurement initially increases and then decreases to form a fluorescence maximum (peak). The time to detection of the fluorescence peak can be used with a correlation schedule to enumerate the SPB in the food sample. A visual test can also be used to identify color changes in the incubation mixture to provide a semi-quantitative enumeration of SPB effective in about 4 hours to 17 hours at 30° C.Type: GrantFiled: December 14, 2001Date of Patent: October 14, 2003Assignee: Colifast ASInventors: Grete Lorentzen, Olaug Taran Skjerdal, James D. Berg
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Patent number: 6511819Abstract: A rapid method for detecting the presence or absence of coliform bacteria in a liquid or liquified dairy sample, for example, skimmed milk, lowfat milk, or whole milk. A growth medium containing a fluorogenic substrate is combined with the dairy sample and is incubated for a brief period of time, for example for about 4 hours, after which a first fluorescence value of 4-methylumbelliferone is measured. The dairy sample is incubated again for about 2-8 hours after which a second fluorescence value of 4-methylumbelliferone is measured. Total, fecal, or thermotolerant coliform bacteria are determined to be present in the sample if the second fluorescent value exceeds the first fluorescent value by a predetermined 4-methylumbelliferone concentration threshold.Type: GrantFiled: March 29, 2001Date of Patent: January 28, 2003Assignee: Nye Colifast ASInventors: Ingun Tryland, James D. Berg, Kari Skjanes
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Patent number: 6165742Abstract: A rapid method for detecting the presence or absence of coliform bacteria in a liquid or liquified dairy sample, for example skimmed milk. A growth medium containing a fluorogenic substrate is combined with the sample and is incubated for a brief period of about 7-9 hours after which a single fluorescence value is measured. Total or thermotolerant coliform bacteria are determined to be present in the sample if the single fluorescent measurement exceeds a predetermined threshold value.Type: GrantFiled: August 12, 1999Date of Patent: December 26, 2000Assignee: NYE Colifast, ASInventors: Gro .O slashed.fjord, Kari Skj.ang.nes, Nina Aalen, James D. Berg
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Patent number: 5972641Abstract: A rapid method for detecting the presence or absence of coliform bacteria in a liquid or liquified dairy sample, for example skimmed milk. A growth medium containing a fluorogenic substrate is combined with the sample and is incubated for a brief period of about 7-9 hours after which a single fluorescence value is measured. Total or thermotolerant coliform bacteria are determined to be present in the sample if the single fluorescent measurement exceeds a predetermined threshold value.Type: GrantFiled: August 28, 1998Date of Patent: October 26, 1999Assignee: Colifast Systems ASAInventors: Gro .O slashed.fjord, Kari Skj.ang.nes, Nina Aalen, James D. Berg