Abstract: The embodiments herein provide methods to analyze the in vitro stability of recalcitrant or membrane-bound proteins in simulated gastric fluid (SGF) comprising the proteolytic enzyme, pepsin, and in combination with a novel pepsin-trypsin assay employing state-of-the-art mass spectrometric approaches, such as LC-MS/MS, to monitor the precise degradation products. The extent of protein digestion can be evaluated by the appearance of peptic products and the disappearance of tryptic peptide products (as a proxy for intact protein). The embodiments herein also provide methods for protein quantitation using high-sensitivity LC-MRM-MS quantification. The methods embodied herein are particularly useful in charactering proteins produced in transgenic plants, such as canola genetically engineered to produce long chain omega-3 polyunsaturated fatty acids.

Abstract: The present invention relates to polypeptides, and polynucleotides encoding therefor, with cation transporter activity. In particular, the present invention relates to methods for producing, identifying, and/or breeding transgenic or non-transgenic plants, especially wheat or barley plants, with enhanced tolerance to saline and/or sodic soils, and/or reduced sodium accumulation in an aerial part of the plant. Also provided are plants produced using these methods.

Abstract: Provided are protein and coding sequences for the ecdysone receptor polypeptide isoforms and ultraspiracle polypeptide from Nezara viridula and methods of employing them.

Abstract: The invention provides excretory/secretory antigens derived from parasitic nematode species which are capable of inducing protective immunity against infection by parasitic nematode species, and related antigenic molecules. The invention also provides nucleotide sequences encoding the antigens and related molecules of the invention, recombinant DNA molecules comprising the nucleotide sequences, and transformed hosts carrying the recombinant DNA molecules. The invention further provides antibodies against the antigens and related molecules, and antibody compositions comprising the antibodies, vaccines comprising the antigens and/or related molecules and methods of treating or preventing nematode infections using the antigens and related molecules, vaccines, antibodies and/or antibody compositions of the invention.

Abstract: An isolated DNA molecule comprising a nucleotide sequence encoding an inducible soft fruit promoter, particularly the alcohol dehydrogenase 2 promoter from tomato, is described. Isolated DNA molecules encoding the alcohol dehydrogenase 2 enzyme are also disclosed.

Abstract: A synthetic double-layered permanganate material including a synthetic double-layered hydroxide material modified such that permanganate anion is included to a level greater than approximately 20% of the theoretical anion exchange capacity of the hydroxide material within its interstices.

Abstract: Packaging apparatus and method, for use in controlled atmosphere packaging of horticultural produce in controlled permeability film. A valve apparatus mounted on or in a packaging film including a temperature sensitive sensor formed from a bimetallic or shape-memory material (2), or a chemical sensitive sensor formed from a swellable polymer, to actuate the valve member (pin or plug) (1) on, or in communication with the sensor. In use, after sealing the produce within the packaging film, the valve apparatus opens the packaging film in response to a temperature change of predetermined magnitude to allow oxygen flow for extension of storage life or fumigation of the produce.

Abstract: A method for protecting proteinaceous fibres, either dyed or in their natural form, against photodegradation and thermal degradation. The method comprises treating the fibres with a sulfonated s-triazine derivative of formula (I), wherein R.sup.1 is hydrogen, alkyl, hydroxyl, O-alky, OOC-alkyl or OOCNH-alkyl; R.sup.2 is hydrogen, alkyl or SO.sub.3 X; R.sup.3 is aryl, substituted aryl or O-alkyl; and X is hydrogen, NH.sub.4 or alkali metal, or formula (II), wherein R.sup.1 and R.sub.4 are hydrogen, alkyl, hydroxyl, O-alkyl, OCC-alkyl or OOCNH-alkyl; R.sup.2 and R.sup.5 are hydrogen, alkyl or --SO.sub.3 X; R.sup.3 is hydrogen or --SO.sub.3 X; R.sup.6 is aryl, substituted aryl, O-alkyl or O-aryl; and X is hydrogen, NH.sub.4 or alkali metal; under acidic conditions. The method is particularly suitable for treating dyed and undyed wool, silk, mohair and cashmere fibers, including blends thereof.