Abstract: The present invention relates to a method and composition for the cryopreservation of adipose tissue with the intention to use this tissue in the culturing of stem and/or progenitor cells. The method uses a specific cryoprotection medium to prevent damage of the original tissue during the cryopreservation while still maintaining a high viability of the stem and/or progenitor cells obtained from the cryopreserved adipose tissue. Furthermore the cryoprotection medium of the present invention does not contain any kind of xenogeneic sera, a critical factor since it is the intention of that the cryopreserved tissue is used for obtaining stem and/or progenitor cells that can be used in medicine. The cryoprotection medium is characterized in that it is a solution of physiological water comprising glycerol and sucrose and/or trehalose and optionally serum albumin.
Type:
Grant
Filed:
March 19, 2009
Date of Patent:
July 9, 2013
Assignee:
Cryo-Save AG
Inventors:
Guy Wouters, Kelly van Wemmel, Peter de Waele
Abstract: The present invention relates to a method and composition for the cryopreservation of adipose tissue with the intention to use this tissue in the culturing of stem and/or progenitor cells. The method uses a specific cryoprotection medium to prevent damage of the original tissue during the cryopreservation while still maintaining a high viability of the stem and/or progenitor cells obtained from the cryopreserved adipose tissue. Furthermore the cryoprotection medium of the present invention does not contain any kind of xenogeneic sera, a critical factor since it is the intention of that the cryopreserved tissue is used for obtaining stem and/or progenitor cells that can be used in medicine. The cryoprotection medium is characterized in that it is a solution of physiological water comprising glycerol and sucrose and/or trehalose and optionally serum albumin.
Type:
Application
Filed:
March 19, 2009
Publication date:
January 13, 2011
Applicant:
CRYO-SAVE AG
Inventors:
Guy Wouters, Kelly van Wemmel, Peter de Waele
Abstract: A large scale storage system of viable somatic stem and/or progenitor cells for use in a method of treatment of a disease or a disorder of a patient is described. The storage system includes a large number of solid supports having cryopreserved/frozen viable somatic stem and/or progenitor cells from patients, and, preoperative information of the patients from which the cells have been taken. A product which includes viable somatic stem and/or progenitor cells combined with preoperative information of the patient from which the somatic stem and/or progenitor cells have been taken is described. The viable somatic stem and/or progenitor cells stored in the described system are useful in treatment of a disease or a disorder.
Type:
Application
Filed:
February 28, 2005
Publication date:
August 9, 2007
Applicant:
CRYO-SAVE AG (FORMERLY LIFE SCIENCES AG)