Abstract: Systems, devices, and methods for localizing magnets are useful for biological tissue analysis and other applications. Tissue analysis systems for use with a tissue analysis device may include a storage medium storing computer readable instructions that cause the execution of magnet localization methods. The system may include the tissue analysis device, which includes a magnet and a magnetometer array disposed adjacent to the magnet. When executed by a processor, the instructions cause operations, including recording a magnetic field of the magnet with the magnetometer array, simulating a simulated magnetic field at each magnetometer of the magnetometer array, and estimating positional information of the magnet based upon iterating simulated positional information of the simulated magnet.

Abstract: A system and process use artificial intelligence to evaluate the toxicity of drugs on cells. In some embodiments, a convolutional neural network is trained to identify features in cells and thereafter identify when structural changes in cells are signs of damage from exposure to a drug. Some embodiments use a 2-class deep neural network, comparing drug-treated cells to controls, to learn which images may show signs of toxicity as a result of the drug. In some applications, the system may capture images from a time-lapse experiment to determine from the cell cultures how a drug affects a cell type over time.

Abstract: Methods and systems for generating three-dimensional (3D) engineered tissues (ETs), and for electrical stimulation of same, are provided. Provided is an ET assembly comprising an ET lid with first post and second post assemblies coupled thereto. Provided is a casting assembly comprising the ET assembly and a casting plate. Provided are stimulation methods and systems for stimulating tissue constructs.

Abstract: Topographically-guided cell migration devices include a substrate, an ion-permeable surface layer having a unidirectional textured first side, and an electrode pair having interdigitated electrode digits disposed between the substrate and the ion-permeable surface layer. The unidirectional texture of the ion-permeable surface layer is configured to guide migration of a cell culture in a substantially linear migration direction. Other topographically-guided cell migration devices include a substrate, a surface layer having a unidirectional textured first side, and a cell confinement structure with two walls that run substantially parallel to the unidirectional texture in order to guide migration of the cell culture in a substantially linear migration direction.