Abstract: An in vivo imaging device having an illumination system that creates a virtual source within a tissue region of a subject in a non-invasive manner. The illumination system transforms a maximum amount of illumination energy from a light source into a high contrast illumination pattern. The illumination pattern is projected onto the object plane in a manner that maximizes the depth to which clear images of sub-surface features can be obtained. The high intensity portion of the illumination pattern is directed onto the object plane outside the field of view of an image capturing device that detects the image. In this configuration, scattered light from within the tissue region interacts with the object being imaged. This illumination technique provides for a high contrast image of sub-surface phenomena such as vein structure, blood flow within veins, gland structure, etc.

Abstract: The invention comprises a system and method of calibrating a reflected spectral imaging apparatus used for analysis of living tissue. In addition to the reflected spectral imaging apparatus itself, the calibration apparatus comprises an optical filter that is placed between the light source used in the imaging apparatus and the object under analysis, and a calibration module. The filter is fabricated such that when the light is passed through the filter, an image is projected onto the focal plane where imaging is to take place within the object. The image projected by the filter comprises a plurality of areas, each having a different known optical density. For each area, the calibration module measures the intensity of the light reflected from the area and maps the light intensity measurement to the optical density known to be present at the area. This correspondence of light intensity measurements and known optical densities is then used to calibrate the reflected spectral imaging apparatus.

Abstract: Method and apparatus for reflected imaging analysis. Reflected imaging is used to perform non-invasive, in vivo analysis of a subject's vascular system. A raw reflected image (110) is normalized with respect to the background to form a corrected reflected image (120). An analysis image (130) is segmented from the corrected reflected image to include a scene of interest for analysis. The method and apparatus can be used to determine such characteristics as the hemoglobin concentration per unit volume of blood, the number of white blood cells per unit volume of blood, a mean cell volume, the number of platelets per unit volume of blood, and the hematocrit. Cross-polarizers can be used to improve visualization of the reflected image.

Abstract: Reflected imaging is used to perform non-invasive, in vivo analysis of a subject's vascular system. A raw reflected image is normalized with respect to the background to form a corrected reflected image. An analysis image is segmented from the corrected reflected image to include a scene of interest for analysis. The method and apparatus can be used to determine such characteristics as the hemoglobin concentration per unit volume of blood, the number of white blood cells per unit volume of blood, a mean cell volume, the number of platelets per unit volume of blood, and the hematocrit. Cross-polarizers can be used to improve visualization of the reflected image.

Abstract: An aspiration needle apparatus for collecting cell samples by withdrawing aspirate from tissue in a living body. An aspiration needle is provided which has a flow passage extending from its proximal extremity to its distal extremity. The proximal extremity has a hub end a cell collection chamber therein in communication with the flow passage. A vacuum container is provided which has an enclosed evacuated space evacuated to a subambient pressure to provide a predetermined volume of evacuated space devoid of a solid or a liquid and devoted solely to providing the desired vacuum. The vacuum container is sealed by a penetrable diaphragm providing access to the evacuated space. An adapter mounted on the hub and making a fluid-tight connection therewith is formed to receive the vacuum container. The adapter includes a needle having a flow passage in communication with the flow passage in the aspiration needle. A spring is carried by the adapter.