Abstract: Disclosed are various embodiments for methods and systems for three-dimensional imaging of subject particles in media through use of dark-field microscopy. Some examples, among others, include a method for obtaining a three-dimensional (3D) volume image of a sample, a method for determining a 3D location of at least one subject particle within a sample, a method for determining at least one spatial correlation between a location of at least one subject particle and a location of at least one cell structure within a cell and/or other similar biological or nonbiological structure, a method of displaying a location of at least one subject particle, method for increasing the dynamic range of a 3D image acquired from samples containing weak and strong sources of light, and method for sharpening a 3D image in a vertical direction.
Type:
Grant
Filed:
November 28, 2018
Date of Patent:
February 23, 2021
Assignee:
CYTOVIVA, INC.
Inventors:
James M. Beach, Samuel M. Lawrence, Byron J. Cheatham, James L. Uertz, Robert P. Dougherty
Abstract: Disclosed are various embodiments for methods and systems for three-dimensional imaging of subject particles in media through use of dark-field microscopy. Some examples, among others, include a method for obtaining a three-dimensional (3D) volume image of a sample, a method for determining a 3D location of at least one subject particle within a sample, a method for determining at least one spatial correlation between a location of at least one subject particle and a location of at least one cell structure within a cell and/or other similar biological or nonbiological structure, a method of displaying a location of at least one subject particle, method for increasing the dynamic range of a 3D image acquired from samples containing weak and strong sources of light, and method for sharpening a 3D image in a vertical direction.
Type:
Grant
Filed:
March 12, 2014
Date of Patent:
December 11, 2018
Assignee:
CYTOVIVA, INC.
Inventors:
James M. Beach, Samuel M. Lawrence, Byron J. Cheatham, James L. Uertz, Robert P. Dougherty