Abstract: Microorganisms are chemically treated with alkali at a pH of 10.5 - 12.9 and a temperature of 0.degree. - 30.degree. C., washed with water and mechanically ruptured at a pH of 7 - 10.2.
Abstract: A method for treating a microorganism which comprises chemically treating the microorganism with at least one agent selected from the group consisting of an acid, an alkali and a hydrophilic solvent and mechanically rupturing the cell membrane of the resulting chemically treated microorganism using a sand grinder or a three roll mill is disclosed.