Abstract: A system for transferring liquid to a multi-well culture plate which includes a machine having a frame upon which is mounted a platform for supporting the culture plate, a transfer plate above the platform and a piston plate above the transfer plate. The transfer plate includes a plurality of chambers closed at the top by a diaphragm. The open bottoms of the chambers may be immersed in a source of the liquid to be transferred on the platform such as a culture plate or reservoir, and the piston plate may be selectively inserted into the chambers of the transfer plate against the diaphragm so that the liquid may be drawn into the chambers from the source. The liquid thereafter may be discharged from the chambers into a multi-well culture plate placed on the platform.