Patents Assigned to DeNovo Sciences
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Patent number: 9856535Abstract: A system and method for isolating cells, comprising: a substrate having a broad surface; an array comprising a set of wells defined at the broad surface of the substrate, each well including: a base surface, an open surface directly opposing the base surface, defined at the broad surface of the substrate, and configured to receive one of a single cell and a single cluster of cells from a direction perpendicular to the broad surface of the substrate, and a set of channels that fluidly couple each well to at least one adjacent well; wherein the set of wells includes an interior subset and an exterior subset fluidly coupled to and surrounding the interior subset by way of the set of channels; and a fluid delivery module surrounding the array and fluidly coupled to each well in the set of wells.Type: GrantFiled: May 28, 2014Date of Patent: January 2, 2018Assignee: DeNovo Sciences, Inc.Inventors: Kalyan Handique, Priyadarshini Gogoi, Yi Zhou, Saedeh Sepehri, Christopher Siemer
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Patent number: 9821311Abstract: A system for isolating cells in at least one of single-cell format and single-cluster format, comprising a reservoir, including a reservoir inlet and a reservoir outlet, configured to receive a biological sample and to receive at least one fluid, a manifold configured to receive and deliver the biological sample and the at least one fluid from the reservoir into a biological sample substrate, the manifold comprising a broad surface comprising a central region configured to receive the biological sample substrate, a set of openings configured to enable fluid flow transmission across the biological sample substrate, a manifold inlet configured to transmit flow from the reservoir the first subset of openings, a manifold outlet configured at a downstream end of the broad surface and coupled to the second subset of openings, the manifold outlet configured to transmit waste fluid from the manifold.Type: GrantFiled: February 14, 2017Date of Patent: November 21, 2017Assignee: DeNovo Sciences, Inc.Inventors: Kalyan Handique, Kyle Gleason, Austin Payne, Priyadarshini Gogoi, Christopher Siemer, Yi Zhou, Saedeh Javdani Sepehri
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Patent number: 9802193Abstract: A system and method for capturing and analyzing cells comprising: a fluid delivery module; a reservoir configured to receive a biological sample including a target cell population and at least one fluid from the fluid delivery module; a manifold configured to receive and distribute the biological sample and at least one fluid from the reservoir into a cell capture device; a waste chamber configured to couple to the manifold; and a pump configured to couple to the waste chamber. In embodiments of the system configured to promote further purification of captured cells, the system can further comprise a magnet that enables further separation of captured cells from undesired sample materials. The system can additionally further comprise a heater configured to heat at least one fluid and/or biological sample, and a cell capture device configured to couple to the manifold, in order to facilitate capture of the target cell population.Type: GrantFiled: February 14, 2017Date of Patent: October 31, 2017Assignee: DeNovo Sciences, Inc.Inventors: Kalyan Handique, Kyle Gleason, Austin Payne, Priyadarshini Gogoi, Saedeh Sepehri Javdani, Christopher Siemer, Yi Zhou
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Patent number: 9752181Abstract: A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells.Type: GrantFiled: January 24, 2014Date of Patent: September 5, 2017Assignee: DeNovo Sciences, Inc.Inventors: Kalyan Handique, Priyadarshini Gogoi, Saedeh Sepehri Javdani, Yi Zhou
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Patent number: 9746413Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.Type: GrantFiled: October 25, 2016Date of Patent: August 29, 2017Assignee: DeNovo Sciences, Inc.Inventors: Kalyan Handique, Priyadarshini Gogoi, Christopher Siemer, Saedeh Sepehri Javdani
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Patent number: 9707562Abstract: A system for isolating cells in at least one of single-cell format and single-cluster format, comprising a reservoir, including a reservoir inlet and a reservoir outlet, configured to receive a biological sample and to receive at least one fluid, a manifold configured to receive and deliver the biological sample and the at least one fluid from the reservoir into a biological sample substrate, the manifold comprising a broad surface comprising a central region configured to receive the biological sample substrate, a set of openings configured to enable fluid flow transmission across the biological sample substrate, a manifold inlet configured to transmit flow from the reservoir the first subset of openings, a manifold outlet configured at a downstream end of the broad surface and coupled to the second subset of openings, the manifold outlet configured to transmit waste fluid from the manifold.Type: GrantFiled: March 18, 2016Date of Patent: July 18, 2017Assignee: DeNovo Sciences, Inc.Inventors: Kalyan Handique, Kyle Gleason, Austin Payne, Priyadarshini Gogoi, Christopher Siemer, Yi Zhou, Saedeh Javdani Sepehri
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Patent number: 9610581Abstract: A system and method for capturing and analyzing cells comprising: a fluid delivery module; a reservoir configured to receive a biological sample including a target cell population and at least one fluid from the fluid delivery module; a manifold configured to receive and distribute the biological sample and at least one fluid from the reservoir into a cell capture device; a waste chamber configured to couple to the manifold; and a pump configured to couple to the waste chamber. In embodiments of the system configured to promote further purification of captured cells, the system can further comprise a magnet that enables further separation of captured cells from undesired sample materials. The system can additionally further comprise a heater configured to heat at least one fluid and/or biological sample, and a cell capture device configured to couple to the manifold, in order to facilitate capture of the target cell population.Type: GrantFiled: September 23, 2015Date of Patent: April 4, 2017Assignee: DeNovo Sciences, Inc.Inventors: Kalyan Handique, Kyle Gleason, Austin Payne, Priyadarshini Gogoi, Saedeh Sepehri Javdani, Christopher Siemer, Yi Zhou
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Patent number: 9612199Abstract: A system for imaging captured cells comprising: an illumination module configured to illuminate a target object; a platform configured to position the target object in relation to the illumination module; a filter module configured to filter light transmitted to the target object and/or to filter light received from the target object, an optical sensor configured to receive light from the target object and to generate image data; and a focusing and optics module configured to manipulate light transmitted to the optical sensor. The system can further comprise one or more of: a control system configured to control at least one of the illumination module, the platform, the focusing and optics module, the filter module, and the optical sensor; a tag identifying system configured to identify and communicate tag information from system elements; a thermal control module configured to adjust temperature parameters of the system; and an image stabilization module.Type: GrantFiled: June 30, 2016Date of Patent: April 4, 2017Assignee: DeNovo Sciences, Inc.Inventors: Kalyan Handique, Kyle Gleason, Christopher Siemer, Priyadarshini Gogoi, Saedeh Sepehri Javdani, Gene Parunak, Aaron Kehrer, Jon Meines
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Patent number: 9606102Abstract: A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells.Type: GrantFiled: January 24, 2014Date of Patent: March 28, 2017Assignee: DeNovo Sciences, Inc.Inventors: Kalyan Handique, Priyadarshini Gogoi, Yi Zhou, Saedeh Sepehri Javdani
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Publication number: 20170073745Abstract: A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells.Type: ApplicationFiled: November 28, 2016Publication date: March 16, 2017Applicant: DeNovo Sciences, Inc.Inventors: Kalyan Handique, Priyadarshini Gogoi, Saedeh Sepehri Javdani, Yi Zhou
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Publication number: 20170038292Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.Type: ApplicationFiled: October 25, 2016Publication date: February 9, 2017Applicant: DeNovo Sciences, Inc.Inventors: Kalyan Handique, Priyadarshini Gogoi, Christopher Siemer, Saedeh Sepehri Javdani
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Patent number: 9513195Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.Type: GrantFiled: January 28, 2015Date of Patent: December 6, 2016Assignee: DeNovo Sciences, Inc.Inventors: Kalyan Handique, Priyadarshini Gogoi, Christopher Siemer, Saedeh Sepehri Javdani
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Patent number: 9404864Abstract: A system for imaging captured cells comprising: an illumination module configured to illuminate a target object; a platform configured to position the target object in relation to the illumination module; a filter module configured to filter light transmitted to the target object and/or to filter light received from the target object, an optical sensor configured to receive light from the target object and to generate image data; and a focusing and optics module configured to manipulate light transmitted to the optical sensor. The system can further comprise one or more of: a control system configured to control at least one of the illumination module, the platform, the focusing and optics module, the filter module, and the optical sensor; a tag identifying system configured to identify and communicate tag information from system elements; a thermal control module configured to adjust temperature parameters of the system; and an image stabilization module.Type: GrantFiled: March 13, 2014Date of Patent: August 2, 2016Assignee: DeNovo Sciences, Inc.Inventors: Kalyan Handique, Kyle Gleason, Christopher Siemer, Priyadarshini Gogoi, Saedeh Sepehri Javdani, Gene Parunak, Aaron Kehrer, Jon Meines
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Patent number: 9174216Abstract: A system and method for capturing and analyzing cells comprising: a fluid delivery module; a reservoir configured to receive a biological sample including a target cell population and at least one fluid from the fluid delivery module; a manifold configured to receive and distribute the biological sample and at least one fluid from the reservoir into a cell capture device; a waste chamber configured to couple to the manifold; and a pump configured to couple to the waste chamber. In embodiments of the system configured to promote further purification of captured cells, the system can further comprise a magnet that enables further separation of captured cells from undesired sample materials. The system can additionally further comprise a heater configured to heat at least one fluid and/or biological sample, and a cell capture device configured to couple to the manifold, in order to facilitate capture of the target cell population.Type: GrantFiled: March 13, 2014Date of Patent: November 3, 2015Assignee: DeNovo Science, Inc.Inventors: Kalyan Handique, Kyle Gleason, Austin Payne, Priyadarshini Gogoi, Saedeh Sepehri Javdani, Christopher Siemer, Yi Zhou
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Patent number: 9103754Abstract: A cell capture system including an array, an inlet manifold, and an outlet manifold. The array includes a plurality of parallel pores, each pore including a chamber and a pore channel, an inlet channel fluidly connected to the chambers of the pores; an outlet channel fluidly connected to the pore channels of the pores. The inlet manifold is fluidly connected to the inlet channel, and the outlet channel is fluidly connected to the outlet channel. A cell removal tool is also disclosed, wherein the cell removal tool is configured to remove a captured cell from a pore chamber.Type: GrantFiled: July 25, 2012Date of Patent: August 11, 2015Assignee: DeNovo Sciences, Inc.Inventors: Kalyan Handique, Priyadarshini Gogoi, Christopher P Siemer, Saedeh Sepehri Javdani
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Publication number: 20140357511Abstract: A system and method for isolating cells, comprising: a substrate having a broad surface; an array comprising a set of wells defined at the broad surface of the substrate, each well including: a base surface, an open surface directly opposing the base surface, defined at the broad surface of the substrate, and configured to receive one of a single cell and a single cluster of cells from a direction perpendicular to the broad surface of the substrate, and a set of channels that fluidly couple each well to at least one adjacent well; wherein the set of wells includes an interior subset and an exterior subset fluidly coupled to and surrounding the interior subset by way of the set of channels; and a fluid delivery module surrounding the array and fluidly coupled to each well in the set of wells.Type: ApplicationFiled: May 28, 2014Publication date: December 4, 2014Applicant: DeNovo SciencesInventors: Kalyan Handique, Priyadarshini Gogoi, Yi Zhou, Saedeh Sepehri, Christopher Siemer
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Publication number: 20140349867Abstract: A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells.Type: ApplicationFiled: January 24, 2014Publication date: November 27, 2014Applicant: DeNovo Sciences, Inc.Inventors: Kalyan Handique, Priyadarshini Gogoi, Yi Zhou, Saedeh Sepehri Javdani
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Publication number: 20140272965Abstract: A system and method for capturing and analyzing cells comprising: a fluid delivery module; a reservoir configured to receive a biological sample including a target cell population and at least one fluid from the fluid delivery module; a manifold configured to receive and distribute the biological sample and at least one fluid from the reservoir into a cell capture device; a waste chamber configured to couple to the manifold; and a pump configured to couple to the waste chamber. In embodiments of the system configured to promote further purification of captured cells, the system can further comprise a magnet that enables further separation of captured cells from undesired sample materials. The system can additionally further comprise a heater configured to heat at least one fluid and/or biological sample, and a cell capture device configured to couple to the manifold, in order to facilitate capture of the target cell population.Type: ApplicationFiled: March 13, 2014Publication date: September 18, 2014Applicant: DeNovo Sciences, Inc.Inventors: Kalyan Handique, Kyle Gleason, Austin Payne, Priyadarshini Gogoi, Saedeh Sepehri Javdani, Christopher Siemer, Yi Zhou
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Publication number: 20140273194Abstract: A system for imaging captured cells comprising: an illumination module configured to illuminate a target object; a platform configured to position the target object in relation to the illumination module; a filter module configured to filter light transmitted to the target object and/or to filter light received from the target object, an optical sensor configured to receive light from the target object and to generate image data; and a focusing and optics module configured to manipulate light transmitted to the optical sensor. The system can further comprise one or more of: a control system configured to control at least one of the illumination module, the platform, the focusing and optics module, the filter module, and the optical sensor; a tag identifying system configured to identify and communicate tag information from system elements; a thermal control module configured to adjust temperature parameters of the system; and an image stabilization module.Type: ApplicationFiled: March 13, 2014Publication date: September 18, 2014Applicant: DeNovo Sciences, Inc.Inventors: Kalyan Handique, Kyle Gleason, Christopher Siemer, Priyadarshini Gogoi, Saedeh Sepehri Javdani
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Publication number: 20140212881Abstract: A system and method for capturing and analyzing a set of cells, comprising: an array including a set of parallel pores, each pore including a chamber including a chamber inlet and a chamber outlet, and configured to hold a single cell, and a pore channel fluidly connected to the chamber outlet; an inlet channel fluidly connected to each chamber inlet of the set of parallel pores; an outlet channel fluidly connected to each pore channel of the set of parallel pores; a set of electrophoresis channels fluidly coupled to the outlet channel, configured to receive a sieving matrix for electrophoretic separation; and a set of electrodes including a first electrode and a second electrode, wherein the set of electrodes is configured to provide an electric field that facilitates electrophoretic analysis of the set of cells.Type: ApplicationFiled: January 24, 2014Publication date: July 31, 2014Applicant: DeNovo Sciences, Inc.Inventors: Kalyan Handique, Priyadarshini Gogoi, Saedeh Sepehri Javdani, Yi Zhou