Abstract: An endophytic fungus from gingko, that is, Fusarium proliferatum DZHQ1 having antitumor activity and antibacterial activity, is isolated from the ginkgo bark. The species of the strain is determined by the combination of colonial morphology and 18 sRNA sequencing, and then the anti-cervical cancer activity of a crude extract of the strain is detected by MTT. Finally, a secondary metabolite of the strain with an inhibition rate of more than 50% is isolated by semi-preparative HPLC, which promotes the further screening of individual compounds with anti-tumor activity. Moreover, the metabolite of the endophytic fungus from gingko shows a more pronounced inhibition in the detection of activity against E. coli and/or S. aureus, and has potential use in the preparation of new antibacterial products.
Abstract: An endophytic fungus from gingko, that is, Fusarium proliferatum DZHQ1 having antitumor activity and antibacterial activity, is isolated from the ginkgo bark. The species of the strain is determined by the combination of colonial morphology and 18 sRNA sequencing, and then the anti-cervical cancer activity of a crude extract of the strain is detected by MTT. Finally, a secondary metabolite of the strain with an inhibition rate of more than 50% is isolated by semi-preparative HPLC, which promotes the further screening of individual compounds with anti-tumor activity. Moreover, the metabolite of the endophytic fungus from gingko shows a more pronounced inhibition in the detection of activity against E. coli and/or S. aureus, and has potential use in the preparation of new antibacterial products.
Abstract: A sensing device and method in detecting binding energy and binding kinetics between molecules; the sensing device has a sensor, a microfluidic chip and a measurement circuit. The sensor has multiple field effect transistors, and each field effect transistor adopts single-layer single crystal graphene as a conductive channel, thereby having extremely high sensitivity and stability; and the field effect transistors are arranged in an array and are provided with multichannel measurement circuits to detect the binding dynamics processes of different molecules or different copies of the same molecule in parallel so as to meet the high-throughput detection requirements. A compound that is non-covalently bound with the single-layer single crystal graphene as a medium to fix probe molecules on the surface of the conductive channel, thereby retaining the intrinsic structure of the graphene, and improving the signal-to-noise ratio and the sensitivity of the graphene field effect transistor.