Abstract: A user-activated, dispenser of pre-moistened, antiseptic towelettes appropriate for hand cleansing use in such contexts as food service, health care, long-term care, child care, educational settings, industry, military, and public restrooms. The dispenser contains two compartments. One is used for towelette-dispensing and the other can be optionally used for housing hand drying means or for storing a reserve supply of towelettes. Each compartment is configured according to operant and classical conditioning strategies that motivate sustained positive changes in both the frequency and effectiveness of hand washing behavior. Optionally, the present invention dispenser can also comprise towelette warming means, a retractable handle, and a sanitary towelette or paper towel disposal assembly.