Abstract: Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.
Type:
Application
Filed:
July 3, 2012
Publication date:
November 1, 2012
Applicant:
E. I. DU PONT DE NEMOURS AND COMPANY & ALLIANCE FOR SUSTAINABLE ENERGY LLC
Inventors:
PERRY G. CAIMI, MARK EMPTAGE, XU LI, PAUL V. VIITANEN, YAT-CHEN CHOU, MARY ANN FRANDEN, MIN ZHANG
Abstract: Identified are mutants of the promoter of the Z. mobilis glyceraldehyde-3-phosphate dehydrogenase gene, which direct improved expression levels of operably linked heterologous nucleic acids. These are high expression promoters useful for expression of chimeric genes in Zymomonas, Zymobacter, and other related bacteria.
Type:
Grant
Filed:
March 25, 2009
Date of Patent:
August 2, 2011
Assignee:
E.I. du Pont de Nemours and Company Alliance for Sustainable Energy LLC
Inventors:
Paul V. Viitanen, Luan Tao, Yuying Zhang, Perry G. Caimi, Laura McCole, Min Zhang, Yat-Chen Chou, Carol M. McCutchen, Mary Ann Franden
Abstract: Strains of xylose utilizing Zymomonas with improved xylose utilization and ethanol production during fermentation in stress conditions were obtained using an adaptation method. The adaptation involved continuously growing xylose utilizing Zymomonas in media containing high sugars, acetic acid, ammonia, and ethanol.
Type:
Application
Filed:
December 18, 2009
Publication date:
January 20, 2011
Applicant:
E.I. DU PONT DE NEMOURS AND COMPANY & ALLIANCE FOR SUSTAINABLE ENERGY LLC
Inventors:
PERRY G. CAIMI, Mark Emptage, Xu Li, Paul V. Viitanen, Yat-Chen Chou, Mary Ann Franden, Min Zhang