Abstract: Removal of the nucleotide sequence encoding the signal peptide from the INGAP coding sequence allows cultured cells to express substantial amounts of INGAP activity. Previous attempts have provided only low yields of INGAP, possibly because the signal sequence of INGAP is toxic to the cells.
Type:
Grant
Filed:
August 12, 1997
Date of Patent:
September 8, 1998
Assignee:
Eastern Virginia Medical School of the Medical College fo Hampton Roads
Inventors:
Aaron I. Vinik, Gary L. Pittenger, Ronit Rafaeloff-Phail, Scott W. Barlow