Abstract: Methods and apparatus for processing eggs based upon a characteristic such as gender are provided. Material is extracted from each of a plurality of live eggs, the extracted material is assayed to identify eggs having the characteristic, and then eggs identified as having the characteristic are processed accordingly.

Abstract: Methods and apparatus for producing vaccine within a plurality of eggs are provided. Each of a plurality of eggs is illuminated with light from one or more LEDs. A detector is positioned adjacent each egg and detects light passing therethrough. Each egg is then identified as containing a live embryo or as being a non-live egg. Each egg that is determined not to contain a live embryo may be removed, either automatically or by hand. A seed virus is injected into each egg identified as containing a live embryo. After a predetermined period of incubation each live embryo is euthanized and amniotic fluid containing a vaccine produced as a result of the presence of a seed virus is harvested from each euthanized egg.

Abstract: A method of determining the gender of a bird in ovo comprises detecting the presence or absence of an elevated level of a sex-related hormone in the extra-embryonic fluid of the bird egg, and then determining the gender of the bird within the egg from the presence of an elevated level of a sex-related hormone therein. In particular embodiments, the sex-related hormone is an estrogen. In other representative embodiments, the invention provides methods in which the extra-embryonic fluid is allantoic fluid. In other illustrative embodiments, the method can be carried out on chicken eggs prior to or during transfer of the eggs from incubator to hatcher.

Abstract: In ovo injection apparatus include an egg carrier configured to hold a plurality of eggs and to provide external access to the eggs, a plurality of injection devices positioned above the carrier, and an egg support assembly positioned beneath the carrier that is configured to support each egg in the carrier during contact therewith by a respective injection device. The egg support assembly includes a frame, a plate having an array of openings attached to the frame, and a plurality of pedestals removably secured within a respective one of the openings. The egg support assembly is operatively associated with the plurality of injection devices such that each pedestal moves upwardly through a respective opening in the carrier to support an egg as a respective injection device makes contact with the egg.

Abstract: Methods and apparatus for identifying live eggs utilize a cup having a photodetector therewithin that overlies a portion of an egg. The egg is illuminated via a light source positioned adjacent the egg. Variations in the intensity of light passing through the egg is detected via the photodetector which generates an output signal corresponding to detected light intensity. The output signal is processed to identify cyclical and non-cyclical variations in light intensity. The egg is designated as a live egg in response to identifying cyclical and/or non-cyclical variations in light intensity.

Abstract: Methods and apparatus for producing vaccine within a plurality of eggs are provided. Each of a plurality of eggs is illuminated with light from one or more LEDs. A detector is positioned adjacent each egg and detects light passing therethrough. Each egg is then identified as containing a live embryo or as being a non-live egg. Each egg that is determined not to contain a live embryo may be removed, either automatically or by hand. A seed virus is injected into each egg identified as containing a live embryo. After a predetermined period of incubation each live embryo is euthanized and amniotic fluid containing a vaccine produced as a result of the presence of a seed virus is harvested from each euthanized egg.

Abstract: Methods of pooling or localizing the allantoic fluid present in avian shell eggs, so that the fluid may be sampled, assessed or injected, are described. The methods involve tilting the avian shell egg so that the major axis of the egg is from about 10 to 180 degrees from the vertically upright position, so that the allantois pools under the uppermost portion of the egg shell. The allantoic fluid may then be sampled, or a substance injected into the allantois, by a vertical needle or probe traveling in a downward direction.

Abstract: An egg transfer apparatus is configured to distribute eggs substantially evenly with reduced breakage on a moving conveyor. The egg transfer apparatus includes a conveyor having opposite first and second ends, a pair of elongated members that extend along the direction of travel of the conveyor between the conveyor first and second ends, and a plurality of spaced-apart, diverging guides positioned downstream from the elongated members. Each elongated member includes opposite first and second end portions. The first end portions of the elongated members are in generally parallel, spaced-apart relationship, and the second end portions have an arcuate configuration and converge to define an opening therebetween through which eggs on the conveyor are conveyed. The diverging guides are positioned downstream from the opening and cause the eggs passing through the opening to be generally evenly distributed at the conveyor second end.

Abstract: An egg transfer apparatus is configured to distribute eggs substantially evenly with reduced breakage on a moving conveyor. The egg transfer apparatus includes a conveyor having opposite first and second ends and a plurality of elongated, flexible guides extending between the conveyor first and second ends in adjacent, spaced apart relationship with each other. The guides define a plurality of channels that are configured to evenly distribute eggs placed on the conveyor. The guides are elevated above the conveyor by an amount that decreases in a direction towards the conveyor second end. In addition, the guides converge toward the conveyor second end.

Abstract: Systems and methods for rapidly identifying live eggs within a stream of eggs with high accuracy, are provided. A stream of eggs are candled via a candling apparatus and each candled egg is designated as being either live, non-live, or “uncertain”. Eggs designated as non-live and uncertain are removed from the stream. Each egg designated as uncertain is “recandled” at a separate station via an additional candling procedure in order to definitively determine if the “uncertain” egg is live or non-live. Uncertain eggs identified as being live via recandling are returned to the egg stream such that the stream contains only live eggs. The uncertain eggs determined to be non-live via recandling are removed and are either discarded or used for other purposes.

Abstract: Methods and apparatus for delivering predetermined dosages of fluid to egg injection devices are provided and include drawing fluid from a fluid source into a syringe barrel via inlet tubing in fluid communication with the fluid source and with the syringe barrel, and dispensing a predetermined dosage of fluid from the syringe barrel to an egg injection device via outlet tubing in fluid communication with the egg injection device and with the syringe barrel. The outlet tubing is closed (e.g., pinched closed) during the drawing step, and the inlet tubing is closed (e.g., pinched closed) during the dispensing step.

Abstract: Methods and apparatus for forming an opening in a shell of an avian egg such that the potential for cracking is greatly reduced are provided and comprise moving a punch through an egg shell at speeds equal to or greater than thirty (30) inches per second. Punches may be driven via various devices including gravity-assisted devices, spring-assisted devices, solenoid-assisted devices, and fluid-assisted (e.g., compressed air, compressed nitrogen, liquid, etc.) devices. The shell of an egg may be pierced without causing cracking by hurling one or more small projectiles at the egg shell at speeds in excess of 30 inches per second. A punch may be mounted on a piezoelectric actuator configured to vibrate at frequencies in a range of between about 1,000 Hz and about 100,000 Hz. These frequencies effectively cause a punch to move at speeds in excess of 30 inches per second.

Abstract: Methods and apparatus that can non-invasively identify the present condition of eggs, namely, whether an egg is a live egg, a clear egg, an early dead egg, a middle dead egg, a late dead egg, a rotted egg, a cracked egg, and/or an inverted egg, are provided. An egg is illuminated with both visible and invisible light at wavelengths of between about three hundred nanometers and about eleven hundred nanometers (300 nm-1,100 nm). Light passing through the egg is received at a detector positioned such that a path of the received light does not pass through an aircell within the egg, except in the case of an inverted egg. The intensity of the light is determined at a plurality of the visible and infrared wavelengths, and a spectrum that represents light intensity at selected ones of the visible and infrared wavelengths is generated. The generated spectrum is compared with one or more spectra associated with a respective known egg condition to identify a present condition of the egg.

Abstract: Apparatus for repositioning eggs from a generally vertical position to a generally horizontal position such that injection/removal of material into/from the side of the egg can occur. Each apparatus may include a cradle, an alignment member, and an orientation member. The cradle has an inclined, arcuate surface with an upper portion, a lower portion, and opposite side portions. The alignment member is operably positioned adjacent the cradle and is configured to engage an egg positioned on the arcuate surface lower portion in an inclined orientation and to releasably secure the egg in a predetermined alignment relative to the cradle. The orientation member is operably positioned relative to the cradle, and is configured to urge an egg positioned on the lower portion in an inclined orientation to a generally vertical orientation after injection/removal of material into/from the egg has occurred.

Abstract: Methods of pooling or localizing the allantoic fluid present in avian shell eggs, so that the fluid may be sampled, assessed or injected, are described. The methods involve tilting the avian shell egg so that the major axis of the egg is from about 10 to 180 degrees from the vertically upright position, so that the allantois pools under the uppermost portion of the egg shell. The allantoic fluid may then be sampled, or a substance injected into the allantois, by a vertical needle or probe traveling in a downward direction.

Abstract: A method of determining the gender of a bird in ovo comprises detecting the presence or absence of an elevated level of a sex-related hormone in the extra-embryonic fluid of the bird egg, and then determining the gender of the bird within the egg from the presence of an elevated level of a sex-related hormone therein. Preferably, the sex-related hormone is an estrogen. Further preferred are methods in which the extra-embryonic fluid is allantoic fluid. The method is preferably carried out on chicken eggs prior to or during transfer of the eggs from incubator to hatcher.

Abstract: Disclosed herein are methods and pharmaceutical formulations for administering vaccines to birds. In preferred embodiments, the invention provides methods of administering live pathogenic virus vaccines to birds in ovo, more preferably, during the last quarter of in ovo incubation. Interferon, more preferably, Type I interferon, may be advantageously administered in conjunction with live virus vaccines to decrease the pathogenicity thereof. Interferon must be provided at doses sufficient to protect against pathogenicity of the live vaccine, but not at doses so high as to prevent the host from mounting an active immune response. Further provided are pharmaceutical formulations comprising effective doses of live vaccine and interferon. Finally, the present invention provides methods of administering interferon together with live vaccines to young avians to effectively overcome the interfering effects of maternal antibodies.

Abstract: An apparatus for classifying a plurality of poultry eggs includes means for detecting the opacities of the eggs, means for detecting the temperatures of the eggs, and means for classifying the eggs using the opacities and the temperatures of the eggs. A method for classifying poultry eggs includes measuring the opacities of the eggs, measuring the temperatures of the eggs, and classifying the eggs as a function of the opacities and the temperatures of the eggs.

Abstract: A multi-site in ovo injection apparatus and related methods for treating live eggs is disclosed. The multi-site injection apparatus includes one or more injection delivery devices which are configured to deliver multiple treatment substances to predetermined areas of eggs. The treatment substances can be provided so that they are spatially and/or temporally separate. The devices and methods enable the effective use of a plurality of treatment substances even those that are effective when used alone but can be noxious if mixed.

Abstract: A method of injecting a plurality of bird eggs comprises: (a) orienting a plurality of avian eggs in a predetermined position; (b) forming an opening in the shell of each of the eggs; (c) extending an elongate delivery device through each of the openings and into the eggs, each of the delivery devices comprising a detector and an injection needle, with the injection needle having a lumen formed therein; (d) detecting with the detector information from the interior of each of the plurality of eggs; and (e) injecting a substance into each of the plurality of eggs through the lumen of said injection needle. The detected information can be used for a variety of purposes, including adjusting the depth of penetration of the injection needle to more precisely control the location of the injection, identifying the gender of the eggs for subsequent sorting of the eggs, distinguishing viable from non-viable eggs, etc.