Abstract: A system and method of applied radial technology chromatography using a plurality of beads is disclosed, with each bead comprising one or more pores therein having a diameter of about 250 ? to about 5000 ?, and each bead having an average radius between about 100 ?m to about 250 ?m. Also disclosed are processes for selecting beads for use in a radial flow chromatography column, and for purifying an unclarified feed stream using a radial flow chromatography column.

Abstract: A system and method of applied radial technology chromatography using a plurality of beads is disclosed, with each bead comprising one or more pores therein having a diameter of about 250 ? to about 5000 ?, and each bead having an average radius between about 100 ?m to about 250 ?m. Also disclosed are processes for selecting beads for use in a radial flow chromatography column, and for purifying an unclarified feed stream using a radial flow chromatography column.

Abstract: The invention provides methods for detecting an analyte in a sample including the steps of: (a) exciting a sensitizer label on an analyte; (b) permitting energy from the excited sensitizer label to be transferred to and excite an acceptor molecule, whereby the sensitizer label returns to an unexcited state; (c) reacting the excited acceptor molecule with a chemiluminescent precursor to form a chemiluminescent compound which emits light in response to an activation source; (d) exposing the chemiluminescent compound to the activating source to produce a detectable signal; (e) detecting the signal; and (f) correlating the signal with the presence or absence of the analyte. The chemiluminescent precursor is desirably an olefin capable of being converted to a 1,2-dioxetane. Target amplification techniques, such as PCR, may be used to directly label a target analyte with a sensitizer.

Abstract: The invention provides chemiluminescent assays that incorporate a film including at least one chemiluminescent precursor immobilized therewith which produces a triggerable chemiluminescent compound, the film being free of compounds which generate singlet oxygen and being adapted for use with a sensitizer-labeled agent or agent probative of the analyte.

Abstract: The invention provides chemiluminescent assays that incorporate a film including at least one chemiluminescent precursor immobilized therewith which produces a triggerable chemiluminescent compound, the film being free of compounds which generate singlet oxygen and being adapted for use with a sensitizer-labeled agent or agent probative of the analyte.

Abstract: The invention provides chemiluminescent assays that incorporate a film including at least one chemiluminescent precursor immobilized therewith which produces a triggerable chemiluminescent compound, the film being free of compounds which generate singlet oxygen and being adapted for use with a sensitizer-labeled agent or agent probative of the analyte.

Abstract: The invention provides methods for detecting an analyte in a sample including the steps of: (a) exciting a sensitizer label on an analyte; (b) permitting energy from the excited sensitizer label to be transferred to and excite an acceptor molecule, whereby the sensitizer label returns to an unexcited state; (c) reacting the excited acceptor molecule with a chemiluminescent precursor to form a chemiluminescent compound which emits light in response to an activation source; (d) exposing the chemiluminescent compound to the activating source to produce a detectable signal; (e) detecting the signal; and (f) correlating the signal with the presence or absence of the analyte. The chemiluminescent precursor is desirably an olefin capable of being converted to a 1,2-dioxetane. Target amplification techniques, such as PCR, may be used to directly label a target analyte with a sensitizer.

Abstract: The invention provides chemiluminescent assays that incorporate a film including at least one chemiluminescent precursor immobilized therewith which produces a triggerable chemiluminescent compound, the film being free of compounds which generate singlet oxygen and being adapted for use with a sensitizer-labeled agent or agent probative of the analyte.

Abstract: The present invention relates to chemiluminescent assays which incorporate a second film or membrane which includes a solid chemical component for activation of a stable dioxetane. Decomposition of the stable dioxetane can be accomplished using a combination of heat and chemical treatment.