Patents Assigned to Fermentas UAB
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Patent number: 8951957Abstract: A composition and method comprising a polycationic agent and a polyanionic agent for delivering a peptide or protein into a cell.Type: GrantFiled: October 11, 2010Date of Patent: February 10, 2015Assignee: Fermentas UABInventors: Arunas Lagunavicius, Lolita Zaliauskiene
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Patent number: 8507239Abstract: Provided is a methylation-specific restriction endonuclease for a DNA duplex substrate, which endonuclease recognizes in a strand of the duplex a 2 to 6 nucleotide recognition sequence comprising a 5-methylcytosine, and cleaves each strand of the duplex at a fixed position outside the recognition sequence.Type: GrantFiled: February 23, 2011Date of Patent: August 13, 2013Assignee: Fermentas UABInventors: Arvydas Lubys, Jolanta Vitkute, Judita Lubiene, Arvydas Janulaitis
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Publication number: 20130034879Abstract: A DNA polymerase mutant comprising a Taq DNA polymerase amino acid sequence with a mutation at one or more of the following selected amino acid positions: E189K, E230K, E507K, H28R, L30R, G38R, F73V, H75R, E76A, E76G, E76K, E90K, K206R, E315K, A348V, L351F, A439T, D452N, G504S, E507A, D551N, L552R, I553V, D578N, H676R, Q680R, D732G, E734G, E734K, F749V; wherein the polymerase mutant exhibits relative to wild-type DNA polymerase increased polymerase speed, increased affinity to DNA substrate and/or increased resistance to a DNA polymerase inhibitor; and wherein, when the mutation is E507K in combination with two or more further mutations or the mutation is Q680R in combination with four or more further mutations, at least one of the further mutations is at one of the selected amino acid positions; and when the mutation is I553V, this is not in combination with D551S.Type: ApplicationFiled: August 2, 2012Publication date: February 7, 2013Applicant: Fermentas UABInventors: Remigijus Skirgaila, Agne Tubeleviciute, Renata Rimseliéné, Sigitas Burinskas
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Publication number: 20120196322Abstract: A method for producing from host cells a heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state. The method cultured the host cells in a culture medium comprising a reducing agent; and recovered the heterologous polypeptide or protein comprising at least one cysteine residue in a reduced state from the host cells or from the culture medium, where the host cells comprise a nucleic acid encoding the heterologous protein or polypeptide, and where the reducing agent is capable of permeating the plasma membrane of the host cells.Type: ApplicationFiled: January 20, 2012Publication date: August 2, 2012Applicant: Fermentas UABInventors: Juozas Siurkus, Peter Neubauer
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Publication number: 20120156752Abstract: The present invention provides a process for the production of nucleic acid encoding a target protein, which comprises: (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: ApplicationFiled: February 29, 2012Publication date: June 21, 2012Applicant: FERMENTAS UABInventors: ARVYDAS JANULAITIS, REMIGIJUS SKIRGAILA, DANGIRA SIKSNIENE
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Publication number: 20110207139Abstract: Provided is a methylation-specific restriction endonuclease for a DNA duplex substrate, which endonuclease recognizes in a strand of the duplex a 2 to 6 nucleotide recognition sequence comprising a 5-methylcytosine, and cleaves each strand of the duplex at a fixed position outside the recognition sequence.Type: ApplicationFiled: February 23, 2011Publication date: August 25, 2011Applicant: Fermentas UABInventors: Arvydas Lubys, Jolanta Vitkute, Judita Lubiene, Arvydas Janulaitis
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Publication number: 20110086794Abstract: A composition and method for delivering a peptide or protein into a cell.Type: ApplicationFiled: October 11, 2010Publication date: April 14, 2011Applicant: FERMENTAS UABInventors: Arunas Lagunavicius, Lolita Zaliauskiene
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Publication number: 20110065606Abstract: The present invention provides a process for the production of nucleic acid encoding a target protein, which comprises: (a) providing an array of RNA or DNA molecules including one or more encoding the target protein; (b) generating a target protein from the array to form RNA-protein or DNA-protein complexes in which the RNA or DNA molecule is non-covalently or covalently bound to the complex; (c) separating the complexes into compartments wherein most or all of the compartments contain no more than one complex; (d) subjecting the complexes to reaction conditions which allow target protein activity; and (e) selecting nucleic acid encoding the target protein on the basis of the activity associated therewith, wherein when the complex is a DNA-protein complex in which the DNA is non-covalently bound, step b) is performed in the absence of separate compartments for each complex.Type: ApplicationFiled: October 7, 2010Publication date: March 17, 2011Applicant: FERMENTAS UABInventors: Arvydas Janulaitis, Remigijus Skirgaila, Dangira Siksniene
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Publication number: 20110059490Abstract: A composition having nucleic acid polymerase activity, which comprises an active nucleic acid polymerase and an excess amount of a non-functional mutant nucleic acid polymerase protein, wherein the non-functional mutant nucleic acid polymerase protein stabilizes the active nucleic acid polymerase against loss of polymerase activity.Type: ApplicationFiled: September 9, 2010Publication date: March 10, 2011Applicant: Fermentas UABInventors: Arunas Lagunavicius, Arvydas Janulaitis
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Patent number: 7811807Abstract: A process for purifying plasmid DNA from a nucleic acid containing sample comprising plasmid DNA and contaminants, which process comprises a step of contaminant removal, comprising: (a) treating the sample to form a nucleic acid solution having a concentration of monovalent cations; (b) contacting the nucleic acid solution with an ultrafiltration membrane having a molecular weight exclusion limit of at least 30 kDa under conditions in which substantially no gel-layer forms and in which the concentration of monovalent cations is sufficiently high for a time sufficient to remove substantially all RNA and form a retentate containing plasmid DNA; and (c) collecting the retentate.Type: GrantFiled: March 27, 2002Date of Patent: October 12, 2010Assignee: Fermentas UABInventors: Rimantas Kvederas, Asta Siksniute, Alyimantas Markauskas
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Patent number: 7799194Abstract: The invention relates to a method of preparing a gel for use in electrophoresis, which method comprises: (a) providing a mixture comprising a solvent, agarose or a derivative thereof, one or more vinyl monomers, and a polymerisation initiator, the initiator forming a redox system with agarose or the derivative thereof; (b) exposing the mixture to polymerisation conditions to graft-polymerise the one or more monomers onto the agarose or derivative thereof; and (c) allowing the mixture to form a gel.Type: GrantFiled: July 16, 2004Date of Patent: September 21, 2010Assignee: Fermentas UABInventor: Ricardas Makuska
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Publication number: 20100041739Abstract: The present invention relates to a polyalkyleneimine polymer having a repeat unit comprising a hydrophilic group, agents containing the same and methods for their use.Type: ApplicationFiled: December 12, 2008Publication date: February 18, 2010Applicant: FERMENTAS UABInventors: Arunas Lagunavicius, Sarunas Zigmantas, Laurynas Riauba, Lolita Zaliauskiene, Richard Makuska, Ausvydas Vareikis, Ula Bernadisiute
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Publication number: 20090202993Abstract: Use of a DNA polymerase enzyme as a single stranded RNA exoribonuclease.Type: ApplicationFiled: October 26, 2007Publication date: August 13, 2009Applicant: Fermentas UABInventors: Arunas Lagunavicius, Arvydas Janulaitis, Zivile Kiveryte, Vilma Zimbaite-Ruskuliene, Agne Savaneviciute
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Publication number: 20090042249Abstract: A vector for transformation into a host cell is described comprising a toxic gene encoding a product that is lethal to the host cell, wherein the toxic gene comprises: an essential sequence region whose integrity is necessary in order for the encoded toxic gene product to be lethal to the host cell; an inessential sequence region whose integrity is not essential in order for the encoded toxic gene product to be lethal to the host cell; a regulatory sequence inserted in-frame into the inessential sequence region; and a cloning site within the essential sequence region for insertion of a nucleic acid sequence, wherein the regulatory sequence and the cloning site are positioned so as to allow the regulatory sequence to be operably linked to a nucleic acid sequence when the nucleic acid sequence is inserted into the cloning site.Type: ApplicationFiled: July 3, 2008Publication date: February 12, 2009Applicant: Fermentas UABInventor: Arvydas Lubys
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Patent number: 6893854Abstract: A process for producing a polynucleotide encoding a restriction endonuclease with an altered specificity, which process comprises: (a) mutagenising a polynucleotide encoding a restriction endonuclease with specificity for a recognition sequence so as to produce one or more mutated polynucleotides; and (b) isolating therefrom a polynucleotide encoding a mutated restriction endonuclease with specificity for an altered recognition sequence by selecting a polynucleotide which expresses a restriction endonuclease with methylase specificity for the altered recognition sequence.Type: GrantFiled: July 18, 2001Date of Patent: May 17, 2005Assignee: Fermentas UABInventors: Arvydas Janulaitis, Renata Rimseliene, Arvydas Lubys