Abstract: A process for the production of hyaluronic acid by continuous fermentation of Streptococcus in a chemostat culture gives high yields of high molecular weight hyaluronic acid uncontaminated by toxic impurities. The process is advantageous in that it solves the problem of traditional batch cultures in which degradation enzymes can begin to break down the cell walls of Streptococcus releasing cells contents into the fermenter broth complicating the purification of high molecular hyaluronic acid.
Type:
Grant
Filed:
January 24, 1995
Date of Patent:
October 8, 1996
Assignee:
Fermentech Medical Limited
Inventors:
Derek C. Ellwood, Charles Gervase T. Evans, Geoffrey M. Dunn, Neil McInnes, Richard G. Yeo, Keith J. Smith
Abstract: A process for the production of hyaluronic acid by continuous fermentation of Streptococcus equi in a chemostat culture gives high yields of high molecular weight hyaluronic acid uncontaminated by toxic impurities. The process is advantageous in that it solves the problem of traditional batch culture in which degradation enzymes can begin to break down the cell walls of Streptococcus releasing cell contents into the fermenter broth, leading to purification difficulties.
Type:
Grant
Filed:
July 6, 1993
Date of Patent:
May 2, 1995
Assignee:
Fermentech Medical Limited
Inventors:
Derek C. Ellwood, Charles G. T. Evans, Geoffrey M. Dunn, Neil McInnes, Richard G. Yeo, Keith J. Smith