Abstract: A process for producing a fibrinogen concentrate for use as a tissue adhesive from mammalian plasma includes freezing the plasma to a temperature of at most about -20.degree. C. for less than four hours in a container having a surface to volume ratio of from about 4.38:1 to about 1.65:1 reciprocal centimeters, thereby producing frozen plasma; thawing the frozen plasma at a temperature of at least about 4.degree. C. for a time sufficient to attain about 5% to about 95% residual icing, thereby producing thawed plasma; and centrifuging the thawed plasma to produce a fibrinogen concentrate having a solids content of about 6% to about 44% solids by weight of the concentrate.

Abstract: Cryoprecipitated mammalian plasma proteins with associated glycoproteins, polysaccharides, and numerous other macromolecular entities are transferred directly in the course of controlled thawing and centrifuging from native plasma phase across the boundary layer into a pre-prepared substrate transfer medium at sustained solidus--liquidus equilibrium regulated to residual icing from about 5 weight percent to about 95 weight percent to produce cryoprecipitates with enhanced productivity and enhanced qualifications for in vivo tissue bonding applications.