Patents Assigned to Fluidigm Canada Inc.
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Patent number: 10705006Abstract: The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.Type: GrantFiled: February 7, 2019Date of Patent: July 7, 2020Assignee: FLUIDIGM CANADA INC.Inventor: Alexandre Loboda
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Patent number: 10669358Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).Type: GrantFiled: July 27, 2018Date of Patent: June 2, 2020Assignee: FLUIDIGM CANADA INC.Inventors: Mitchell A. Winnik, Mark Nitz, Vladimir Baranov, Xudong Lou
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Publication number: 20200140800Abstract: Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer or mass spectrometer.Type: ApplicationFiled: October 2, 2019Publication date: May 7, 2020Applicant: Fluidigm Canada Inc.Inventor: Alexander V. Loboda
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Patent number: 10622199Abstract: The invention relates to methods and devices for analysis of samples using laser ablation imaging mass cytometry and mass spectrometry. The invention provides methods and devices in which individual ablation plumes are distinctively captured and rapidly transferred to the ionization system, followed by analysis by mass spectrometry. A transfer conduit can be used to convey ablation plumes to an ionization system. The transfer conduit can include an asymmetric cone. The transfer conduit can be tapered. A flow sacrificing system can be adapted to divert a part of the sheath flow out a sacrificial outlet while the core of the sheath flow containing ablation plumes enters the ionization system.Type: GrantFiled: August 2, 2017Date of Patent: April 14, 2020Assignee: FLUIDIGM CANADA INC.Inventor: Alexander V. Loboda
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Patent number: 10598584Abstract: The inventors have improved mass cytometer to facilitate its use for the analysis of particles.Type: GrantFiled: October 19, 2018Date of Patent: March 24, 2020Assignee: FLUIDIGM CANADA INC.Inventors: Alexander V. Loboda, Dmitry R. Bandura, Vladimir I. Baranov
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Patent number: 10577648Abstract: The invention relates to the use of inductively coupled plasma mass spectroscopy for cellular sample analysis. In some embodiments a method of performing mass spectroscopy analysis using an inductively coupled plasma mass spectroscopy system is provided. The method may include introducing a cellular sample comprising one or more cells or cellular particles into an inductively coupled plasma of the inductively coupled plasma mass spectroscopy system. The method may further include using the inductively coupled plasma mass spectroscopy system to assess the cellular sample by detecting and measuring one or more element tags in the cellular sample based on the element or isotopic compositions of the one or more element tags.Type: GrantFiled: August 11, 2015Date of Patent: March 3, 2020Assignee: FLUIDIGM CANADA INC.Inventor: Olga Ornatsky
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Patent number: 10472600Abstract: Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer or mass spectrometer.Type: GrantFiled: April 30, 2018Date of Patent: November 12, 2019Assignee: FLUIDIGM CANADA INC.Inventor: Alexander V. Loboda
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Publication number: 20190317082Abstract: Analyzing samples injected into an inductively coupled plasma source can be improved by one or more of a stabilizing solution mixable with a sample prior to injection and a heated injector. The stabilizing solution can minimize the difference in osmotic pressure between the solution and the cells with a relatively low amount of dissolved solids (e.g., at or below about 0.2%). The stabilizing solution can contain a salt (e.g., ammonium nitrate) present in concentrations of at least 5 mM. The injector can be heated before and/or during injection. In some cases, heat from adjacent parts can be channeled into the injector to improve heating of the injector. An injector heated to sufficient temperatures can minimize solute buildup and can extend the usable time between cleanings. These improvements can be especially useful in elemental analysis, such as inductively coupled plasma mass spectrometry or inductively coupled plasma optical emission spectrometry.Type: ApplicationFiled: April 12, 2019Publication date: October 17, 2019Applicant: Fluidigm Canada Inc.Inventors: Vladimir Baranov, Alexander Loboda, Michael Sullivan
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Publication number: 20190180996Abstract: The invention relates to methods and devices for analysis of samples using laser ablation imaging mass cytometry and mass spectrometry. The invention provides methods and devices in which individual ablation plumes are distinctively captured and rapidly transferred to the ionization system, followed by analysis by mass spectrometry. A transfer conduit can be used to convey ablation plumes to an ionization system. The transfer conduit can include an asymmetric cone. The transfer conduit can be tapered. A flow sacrificing system can be adapted to divert a part of the sheath flow out a sacrificial outlet while the core of the sheath flow containing ablation plumes enters the ionization system.Type: ApplicationFiled: August 2, 2017Publication date: June 13, 2019Applicant: Fluidigm Canada Inc.Inventor: Alexander V. LOBODA
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Patent number: 10241023Abstract: The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.Type: GrantFiled: April 18, 2018Date of Patent: March 26, 2019Assignee: Fluidigm Canada Inc.Inventor: Alexandre Loboda
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Publication number: 20190077890Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).Type: ApplicationFiled: July 27, 2018Publication date: March 14, 2019Applicant: FLUIDIGM CANADA INC.Inventors: MITCHELL A. WINNIK, MARK NITZ, VLADIMIR BARANOV, XUDONG LOU
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Patent number: 10139332Abstract: The inventors have improved mass cytometer to facilitate its use for the analysis of particles.Type: GrantFiled: December 29, 2015Date of Patent: November 27, 2018Assignee: Fluidigm Canada Inc.Inventors: Alexander V. Loboda, Dmitry R. Bandura, Vladimir I. Baranov
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Publication number: 20180312794Abstract: Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer or mass spectrometer.Type: ApplicationFiled: April 30, 2018Publication date: November 1, 2018Applicant: Fluidigm Canada Inc.Inventor: Alexander V. Loboda
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Patent number: 10107734Abstract: A combination of mutually exclusive cell-based analytical techniques can be applied to the same group of cells for analysis. The same group of cells can be prepared for analysis by each technique resulting with candidate cells targeted for mass cytometry analysis. This configuration allows for the correlation of the information between each technique to produce a matrix of multi dimension of cellular information with the same group of cells.Type: GrantFiled: August 8, 2016Date of Patent: October 23, 2018Assignee: Fluidigm Canada Inc.Inventor: Vladimir Baranov
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Patent number: 10072104Abstract: Element tags based on novel metal-polymer conjugates are provided for elemental analysis of analytes, including ICP-MS. A polymer backbone is functionalized to irreversibly bind metals that are selected prior to use by the user. The polymer is further functionalized to attach a linker which allows for attachment to antibodies or other affinity reagents. The polymer format allows attachment of many copies of a given isotope, which linearly improves sensitivity. The metal-polymer conjugate tags enable multiplexed assay in two formats: bulk assay, where the average biomarker distribution in the sample is diagnostic, and single cell format to distinguish a rare (for example a diseased) cell in a complex sample (for example, blood).Type: GrantFiled: October 5, 2015Date of Patent: September 11, 2018Assignee: Fluidigm Canada Inc.Inventors: Mitchell A. Winnik, Mark Nitz, Vladimir Baranov, Xudong Lou
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Patent number: 9963667Abstract: Apparatus and methods for delivering biological samples to an ICP source of a mass cytometer are disclosed. Biological material is disposed on a plurality of discrete sites on a carrier. The plurality of discrete sites are configured to retain biological material and to release the biological material upon application of energy. The carrier is positioned in proximity to a gas conduit and upon release from the discrete sites, the biological material becomes entrained in a gas flow, which delivers discrete portions of biological material through the conduit to the ICP source for analysis by mass cytometry. The apparatus and methods can provide a continuous stream of discrete portions of biological material to a mass cytometer or mass spectrometer.Type: GrantFiled: December 31, 2015Date of Patent: May 8, 2018Assignee: Fluidigm Canada Inc.Inventor: Alexander V. Loboda
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Patent number: 9952132Abstract: The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.Type: GrantFiled: January 18, 2017Date of Patent: April 24, 2018Assignee: Fluidigm Canada Inc.Inventor: Alexandre Loboda
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Patent number: 9874567Abstract: Methods and kits for enzymes involved in post-translational modifications are provided. The methods employ elemental analysis, including ICP-MS. The methods allow for the convenient and accurate analysis of post-translation modifications of substrates by enzymes involved in post-translational modifications, including kinase and phosphatase enzymes. Kits may include an element tag for directly tagging an affinity product that recognizes phosphorylated substrates, as well as a non-phosphorylated substrate or an expression plasmid.Type: GrantFiled: February 2, 2016Date of Patent: January 23, 2018Assignee: Fluidigm Canada Inc.Inventor: Olga Ornatsky
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Patent number: 9632079Abstract: The invention relates to methods and devices for molecular cytometry. The molecular cytometer is for use in the analysis of molecular tag labeled particles. In the molecular cytometer, volatile molecular tags attached to the particles, such as cells, are released as groups of molecular tags and ionized by the method of soft ionization to produce corresponding groups of molecular ions. The molecular cytometer has two detectors, one to detect the presence of the groups of molecular ions and the other to detect mobility separated molecular ions. The mobility separated molecular ions are synchronized to correspond with the groups of molecular ions.Type: GrantFiled: May 22, 2014Date of Patent: April 25, 2017Assignee: Fluidigm Canada Inc.Inventors: Dmitry R. Bandura, Vladimir I. Baranov, Alexander V. Loboda, Olga Ornatsky, Scott D. Tanner
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Patent number: 9589779Abstract: The invention relates to methods and devices for analysis of samples using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The invention provides methods and devices in which individual ablation plumes are distinctively captured and transferred to the ICP, followed by analysis by mass cytometry.Type: GrantFiled: April 17, 2014Date of Patent: March 7, 2017Assignee: Fluidigm Canada Inc.Inventor: Alexandre Loboda