Abstract: The invention relates to the use of deglycosylated detectors, especially secondary antibodies, for the determination of sugar structures of proteins, especially recombinant proteins. It further relates to the use of deglycosylated enzymes in the determination of sugar structures with the aid of an enzyme-substrate reaction; the invention further relates to a method of determining the sugar structures of proteins, a sugar determination kit and the use of said kit for the determination of sugar structures, especially of recombinant therapeutic proteins, preferably immunoglobulins.
Type:
Grant
Filed:
September 11, 2008
Date of Patent:
April 2, 2013
Assignee:
Galab Technologies GmbH
Inventors:
Jürgen Kuballa, Kirstin Umnus, Simone Cartellieri
Abstract: The invention relates to the use of deglycosylated detectors, especially secondary antibodies, for the determination of sugar structures of proteins, especially recombinant proteins. It further relates to the use of deglycosylated enzymes in the determination of sugar structures with the aid of an enzyme-substrate reaction; the invention further relates to a method of determining the sugar structures of proteins, a sugar determination kit and the use of said kit for the determination of sugar structures, especially of recombinant therapeutic proteins, preferably immunoglobulins.
Type:
Application
Filed:
September 11, 2008
Publication date:
August 26, 2010
Applicant:
GALAB TECHNOLOGIES GMBH
Inventors:
Jürgen Kuballa, Kirstin Umnus, Simone Cartellieri