Patents Assigned to Gene Stream Pty Ltd.
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Patent number: 11078519Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.Type: GrantFiled: June 13, 2019Date of Patent: August 3, 2021Assignee: GENE STREAM PTY LTD.Inventors: Marco Peter Leu, John Michael Daly
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Patent number: 10428317Abstract: Luciferase polypeptides with improved light-emitting activity and their encoding nucleic acids are disclosed. These molecules are useful in a range of assays including luciferase-based gene reporter assays, bioluminescence resonance energy transfer assays, protein complementation assays and other applications in which luciferase enzymes are utilized as detectable and/or quantifiable labels. Methods and compositions for increasing the sensitivity and/or improving the kinetics of luciferase-catalyzed reactions as well as decreasing the impact of undesirable variables are also disclosed.Type: GrantFiled: December 5, 2011Date of Patent: October 1, 2019Assignee: Gene Stream Pty LtdInventors: John Daly, Leon Michael Brownrigg, Jim Yu-Hsiang Tiao
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Patent number: 10364454Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analyzed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.Type: GrantFiled: December 30, 2016Date of Patent: July 30, 2019Assignee: GENE STREAM PTY LTD.Inventors: Marco Peter Leu, John Michael Daly
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Patent number: 9994858Abstract: The present invention relates generally to constructs and their use in gene expression or gene regulation assays. More particularly, the present invention provides expression vectors and/or reporter vectors providing kinetics of protein expression with improved temporal correlation to promoter activity. Even more particularly, the invention provides expression vectors comprising a transcribable polynucleotide which comprises a sequence of nucleotides encoding a RNA element that modulates the stability of a transcript corresponding to the transcribable polynucleotide. The present invention provides, inter alia, novel vectors, useful for identifying and analysing cis- and trans-acting regulatory sequences/factors as well as vectors and genetically modified cell lines or organisms that are particularly useful for drug screening and drug discovery.Type: GrantFiled: October 4, 2006Date of Patent: June 12, 2018Assignee: Gene Stream PTY LTDInventor: John Daly
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Publication number: 20170211126Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.Type: ApplicationFiled: December 30, 2016Publication date: July 27, 2017Applicant: GENE STREAM PTY LTD.Inventors: Marco Peter Leu, John Michael Daly
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Patent number: 9567624Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analyzed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.Type: GrantFiled: March 17, 2015Date of Patent: February 14, 2017Assignee: Gene Stream Pty Ltd.Inventors: Marco Peter Leu, John Michael Daly
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Patent number: 8399212Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.Type: GrantFiled: December 21, 2007Date of Patent: March 19, 2013Assignee: Gene Stream Pty LtdInventors: Marco Peter Leu, John Michael Daly
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Patent number: 8232047Abstract: Reagents and compositions for use in reactions catalysed by luciferase enzymes, and in particular for use in luciferase-based gene reporter assays are described. The invention also provides methods and compositions for, inter alia, increasing the sensitivity and/or improving the kinetics of luciferase-catalysed reactions.Type: GrantFiled: October 24, 2007Date of Patent: July 31, 2012Assignee: Gene Stream Pty Ltd.Inventors: Marco Peter Leu, John Michael Daly
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Publication number: 20100092967Abstract: Disclosed herein are methods for determining the amount or activity of one or more luciferases and methods for measuring the luminescent signal generated by one or more luciferases in a sample, the methods comprising incubating the sample with a reactive substrate(s) of the luciferase(s) to be analysed and a reducing agent to inactivate a first luciferase, wherein the first luciferase, in its native form, is a secreted luciferase.Type: ApplicationFiled: December 21, 2007Publication date: April 15, 2010Applicant: GENE STREAM PTY LTDInventors: Marco Peter Leu, John Michael Daly
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Publication number: 20100055693Abstract: Reagents and compositions for use in reactions catalysed by luciferase enzymes, and in particular for use in luciferase-based gene reporter assays are described. The invention also provides methods and compositions for, inter alia, increasing the sensitivity and/or improving the kinetics of luciferase-catalysed reactions.Type: ApplicationFiled: October 24, 2007Publication date: March 4, 2010Applicant: GENE STREAM PTY LTD.Inventors: Marco Peter Leu, John Michael Daly
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Patent number: 7157272Abstract: The present invention relates generally to constructs and their use in gene expression or gene regulation assays. More particularly, the present invention provides expression vectors and/or reporter vectors providing kinetics of protein expression with improved temporal correlation to promoter activity. Even more particularly, the invention provides expression vectors comprising a transcribable polynucleotide which comprises a sequence of nucleotides encoding a RNA element that modulates the stability of a transcript corresponding to the transcribable polynucleotide. The present invention provides, inter alia, novel vectors, useful for identifying and analysing cis- and trans-acting regulatory sequences/factors as well as vectors and genetically modified cell lines or organisms that are particularly useful for drug screening and drug discovery.Type: GrantFiled: September 9, 2003Date of Patent: January 2, 2007Assignee: Gene Stream Pty Ltd.Inventor: John Daly
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Publication number: 20040209274Abstract: The present invention relates generally to constructs and their use in gene expression or gene regulation assays. More particularly, the present invention provides expression vectors and/or reporter vectors providing kinetics of protein expression with improved temporal correlation to promoter activity. Even more particularly, the invention provides expression vectors comprising a transcribable polynucleotide which comprises a sequence of nucleotides encoding a RNA element that modulates the stability of a transcript corresponding to the transcribable polynucleotide. The present invention provides, inter alia, novel vectors, useful for identifying and analysing cis- and trans-acting regulatory sequences/factors as well as vectors and genetically modified cell lines or organisms that are particularly useful for drug screening and drug discovery.Type: ApplicationFiled: September 9, 2003Publication date: October 21, 2004Applicant: Gene Stream Pty LtdInventor: John Daly
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Patent number: RE46199Abstract: Reagents and compositions for use in reactions catalysed by luciferase enzymes, and in particular for use in luciferase-based gene reporter assays are described. The invention also provides methods and compositions for, inter alia, increasing the sensitivity and/or improving the kinetics of luciferase-catalysed reactions.Type: GrantFiled: July 31, 2014Date of Patent: November 8, 2016Assignee: GENE STREAM PTY LTD.Inventors: Marco Peter Leu, John Michael Daly
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Patent number: RE47607Abstract: Reagents and compositions for use in reactions catalysed by luciferase enzymes, and in particular for use in luciferase-based gene reporter assays are described. The invention also provides methods and compositions for, inter alia, increasing the sensitivity and/or improving the kinetics of luciferase-catalysed reactions.Type: GrantFiled: October 13, 2016Date of Patent: September 17, 2019Assignee: GENE STREAM PTY LTD.Inventors: Marco Peter Leu, John Michael Daly