Abstract: The invention relates to methods for capturing a polynucleotide comprising a DNA target segment from a population of polynucleotide molecules. The method may use a mixture comprising the population of the polynucleotide molecules, one or more polynucleotide primers, nucleotides, biotinylated nucleotides, a polymerase enzyme and a buffered liquid medium. The method may denature the polynucleotide molecules in the mixture so as to create single stranded portions of the polynucleotide molecules which can be accessible to the polynucleotide primers; hybridizing the polynucleotide primers to the polynucleotide comprising the DNA target segment. The method may create an extended polynucleotide primer by using the nucleotides, the biotinylated nucleotides and the polymerase enzyme as a means for labeling with biotin and increasing the extent of hybridization of the extended polynucleotide primer to the polynucleotide comprising the DNA target segment.
Type:
Application
Filed:
July 11, 2023
Publication date:
January 25, 2024
Applicant:
Generation Biotech, LLC
Inventors:
Johannes Dapprich, Karl P. Dresdner, Jr., Richard R. McKay
Abstract: The invention relates to methods for capturing a polynucleotide comprising a DNA target segment from a population of polynucleotide molecules using a rotation of a magnetic field to move streptavidin-labeled magnetic beads relative to a buffered liquid medium to increase the probability of capturing and preventing damage to a long polynucleotide comprising a DNA target segment. The methods may use a rotation of the magnetic field relative to the mixture or a rotation of the mixture relative to the magnetic field for causing magnetic bead rotation in the buffered liquid medium and increasing the binding of biotinylated nucleotides of an extended polynucleotide primer to streptavidin-labeled magnetic beads, and winding or condensation of a polynucleotide onto the streptavidin-labeled magnetic beads to prevent damage to the long polynucleotide comprising a DNA target segment.
Type:
Grant
Filed:
May 29, 2019
Date of Patent:
November 21, 2023
Assignee:
GENERATION BIOTECH, LLC
Inventors:
Johannes Dapprich, Karl P. Dresdner, Jr., Richard R. McKay
Abstract: The invention relates to methods for capturing a polynucleotide comprising a DNA target segment from a population of polynucleotide molecules. The method may use a mixture comprising the population of the polynucleotide molecules, one or more polynucleotide primers, nucleotides, biotinylated nucleotides, a polymerase enzyme and a buffered liquid medium. The method may denature the polynucleotide molecules in the mixture so as to create single stranded portions of the polynucleotide molecules which can be accessible to the polynucleotide primers; hybridizing the polynucleotide primers to the polynucleotide comprising the DNA target segment. The method may create an extended polynucleotide primer by using the nucleotides, the biotinylated nucleotides and the polymerase enzyme as a means for labeling with biotin and increasing the extent of hybridization of the extended polynucleotide primer to the polynucleotide comprising the DNA target segment.
Type:
Application
Filed:
May 29, 2019
Publication date:
March 12, 2020
Applicant:
Generation Biotech, LLC
Inventors:
Johannes Dapprich, Karl P. Dresdner, JR., Richard R. McKay
Abstract: The present invention provides methods and compositions for sequence-specific isolation of polynucleotide molecules from nucleic acid populations and subsequent amplification of isolated polynucleotide molecules or fragments thereof.
Abstract: The invention is directed to methods to identify the location in a genome of a nonfixed or multicopy genomic element using microarrays or sequencing.
Type:
Application
Filed:
May 14, 2007
Publication date:
October 22, 2009
Applicants:
Generation Biotech, LLC, Rutgers, the State University, Princeton University
Inventors:
Johannes Dapprich, Abram Gabriel, Maitreya Dunham
Abstract: The present invention provides methods and compositions for sequence-specific isolation of polynucleotide molecules from nucleic acid populations and subsequent amplification of isolated polynucleotide molecules or fragments thereof.
Abstract: The present invention provides methods and compositions for sequence-specific isolation of polynucleotide molecules from nucleic acid populations and subsequent amplification of isolated polynucleotide molecules or fragments thereof.