Abstract: A method for detecting in vitro the presence of a genome of a DNA virus or a viral derived DNA in an infected eukaryotic cell, tissue or biological fluid using Molecular Combing or other nucleic acid stretching methods together with probes, especially nucleic acid probes, having a special design. A method for monitoring in vitro the effects of anti-viral treatment by following the presence of genomic viral or viral derived DNA polynucleotides in a virus-infected cell, tissue or biological fluid. Detection of an infectious form of a virus using Molecular Combing and DNA hybridization. A kit comprising probes used to carry out these methods and a composition comprising the probes.

Abstract: The invention involves methods for identification of biomarkers of the severity of an HPV infection.

Abstract: The present invention, called Physical Characterization of Telomere (PCT), provides and advantageous, accurate and convenient new methods for the visualization, characterization and measurements of telomere sequences. It employs probes and dyes to create a pattern of physical images, classifies the images, and determines the lengths of telomere sequences. PCT brings to a deeper understanding of telomere modifications that occur either genome wide manner or in a chromosome specific way.

Abstract: The invention concerns the detection and the quantification of integrated nucleic acids of viruses and thus the detection and follow-up of reservoir cells harbouring such integrated viral genomes. One aspect of the invention is directed to a method for detecting a level of integrated viral DNA that includes removing episomal viral or vector nucleic acids from genomic DNA in a cell sample, and quantifying a number of integrations of viral DNA into the genomic DNA of the cells by a method of amplification of an integration region in the DNA sample; thereby detecting a level of integrated viral DNA in the genomic DNA from a cell sample, such as a biological sample containing HPV virus and DNA.

Abstract: Methods for detecting and characterizing large genomic rearrangements induced by modified nucleases at high resolution and for quantifying the frequency of the large genomic or gene rearrangements induced by modified nucleases using Molecular Combing.

Abstract: The invention involves methods for identification of biomarkers of the severity of an HPV infection.

Abstract: A method for detecting in vitro the presence of a genome of a DNA virus or a viral derived DNA in an infected eukaryotic cell, tissue or biological fluid using Molecular Combing or other nucleic acid stretching methods together with probes, especially nucleic acid probes, having a special design. A method for monitoring in vitro the effects of anti-viral treatment by following the presence of genomic viral or viral derived DNA polynucleotides in a virus-infected cell, tissue or biological fluid. Detection of an infectious form of a virus using Molecular Combing and DNA hybridization. A kit comprising probes used to carry out these methods and a composition comprising the probes.

Abstract: An automated, computer-implemented method for detecting and measuring large amounts of data obtained from molecular combing procedures including the identification and characterization of seven different DNA replication initiation and termination patterns.

Abstract: The invention involves methods for identification of biomarkers of the severity of an HPV infection.

Abstract: The present invention is related to a method of identifying at least one sequence of target regions on a plurality of macromolecules to test, each target region being associated with a tag and said macromolecules having underwent linearization according to a predetermined direction, wherein said method comprises performing by a processor (11) of equipment (10) the following steps: (a) receiving from a scanner (2) being sensitive to said tags, at least one sample image depicting said macromolecules as curvilinear objects sensibly extending according to said predetermined direction; (b) Generating a binary image from the sample age; (c) For at least one template image, and for each sub-area of the binary image having the same size as the template image, calculating a correlation score between the sub-area and the template image; (d) For each sub-area of the binary image for which the correlation score with a template image is above a first given threshold, selecting the corresponding sub-area of the sample imag

Abstract: A molecular-combing, Genetic-Morse Code based method enabling the detection and high-resolution characterization of complex regions of genomic DNA, such as the SMA locus, with molecular combing. A method for the identification of biomarkers associated to the cis-duplication of SMN1 gene or segments of other complex parts of the genome. Biomarkers identified by this method which are composed of a sets of different colored probes, such as those disclosed for the SMA region.

Abstract: The present invention is related to a method of analyzing a set of sequences of target regions on a plurality of macromolecules to test so as to detect anomalies therein, each target region being associated with a tag and said macromolecules having underwent linearization according to a predetermined direction, wherein said method comprises performing by a processor (11) of equipment (10) the following steps: (a) Identifying said sequences of target regions from at least one sample image received from a scanner (2), said sample image depicting said macromolecules as curvilinear objects sensibly extending according to said predetermined direction; (b) Determining if there is at least one target region presenting a bimodal distribution of the lengths of said target region; (c) Determining if there is at least one recurrent breakpoint position in said sequences of target regions; If at least one target region presenting a bimodal distribution of length and/or at least one recurrent breakpoint position has been d

Abstract: Methods for detecting genomic rearrangements in BRCA1 and BRCA2 genes at high resolution using Molecular Combing and for determining a predisposition to a disease or disorder associated with these rearrangements including predisposition to ovarian cancer or breast cancer. Primers useful for producing probes for this method and kits for practicing the methods.

Abstract: Methods for detecting the amplifications of sequences in the BRCA1 locus, which sequences have ends consisting of or are framed with sequence stretches present at least twice in the BRCA1 locus, and which amplification results in at least two or at least three, especially three, tandem copies of the amplified sequence; methods for determining a predisposition to diseases or disorders associated with these amplifications, including predisposition to ovarian cancer or breast cancer and methods for detecting amplifications with similar features in other loci.

Abstract: Methods including in-silico steps for design and synthesis of Genomic Morse Code (“GMC”) probes including design of combinations of polynucleotide sequences and labelling colors for analysis of large rearrangements in targeted genetic regions as well as allele characterization of complex regions and localization of events such as replication, DNA reparation or epigenetics in particular regions. Color-encoded sets of probes that produce characteristic or unique color patterns when painted on a target nucleic acid sequence. Methods for using color-encoded sets of probes.

Abstract: Methods for detecting and characterizing large genomic rearrangements induced by modified nucleases at high resolution and for quantifying the frequency of the large genomic or gene rearrangements induced by modified nucleases using Molecular Combing.

Abstract: A method for detection, visualization and/or comparison of polynucleotide sequences of interest using specially designed sets of long and short probes that enhance resolution and simplify visualization and detection. Probe compositions useful for practicing this method and procedures for identifying useful probes and probe combinations. These methods are useful for the detection of genomic rearrangements, especially those associated with various diseases, disorders and conditions including cancer or for assessment of genomic rearrangements associated with therapy. The probe compositions may be used in kits for detection of genetic rearrangements or in companion diagnostic products or kits, such as kits for the diagnosis or assessment of predisposition to cancer such as colorectal cancer.

Abstract: Methods and products for detecting in vitro the presence of damage on DNA or the presence of a biological response to damage on DNA at the molecular level. Molecular Combing or other nucleic acid stretching methods are employed together with compounds reacting with DNA, probes binding DNA, or nucleic acid monomers, especially labeled nucleic acid monomers.

Abstract: The present invention relates to a method for analyzing in vitro D4Z4 tandem repeat arrays of nucleic acid contained on nucleic acid representative of chromosomes, in particular on nucleic acid representative of Human chromosomes 4 and 10, and to a kit therefore. Said method is in particular suitable for determining the number of D4Z4 repeat units in said D4Z4 repeat arrays. Said method is based on stretching of nucleic acid and in particular on Molecular Combing and relies on the use of probes, especially nucleic acid probes, with a particular design. The invention also relates to a method for providing tools for the diagnosis of facioscapulohumeral muscular dystrophy (FSHD) and to a diagnostic kit therefore. The invention further relates to a method for identifying biochemical events and/or genetic in regions containing such tandem repeat arrays.

Abstract: A method for detecting in vitro the presence of a genome of a DNA virus or a viral derived DNA in an infected eukaryotic cell, tissue or biological fluid using Molecular Combing or other nucleic acid stretching methods together with probes, especially nucleic acid probes, having a special design. A method for monitoring in vitro the effects of anti-viral treatment by following the presence of genomic viral or viral derived DNA polynucleotides in a virus-infected cell, tissue or biological fluid. Detection of an infectious form of a virus using Molecular Combing and DNA hybridization. A kit comprising probes used to carry out these methods and a composition comprising the probes.