Abstract: This invention relates to the use of a unique apparatus and method for extracting radioactive components from liquids, such as electrophoresis buffers. In the present invention, the radioactive liquid is pumped or transported by other suitable means and is passed through a cartridge having an elongated chamber that contains an exchange medium. The exchange medium, i.e. exchange resin, is contained within the elongated chamber between an upper frit and a lower frit. Further, the cartridge has a means within the elongated chamber for retaining pressure between the upper frit and the lower frit. The nucleotides, being charged molecules, bind to the exchange medium and are removed from the liquid. The liquid then exits the cartridge and has such a low amount of radioactivity that it can be disposed of to a conventional drain.
Abstract: A system for identification of DNA bases in a nucleotide sequence includes a microprocessor and a set of executable instructions and is configured to accept a set of input data corresponding to a DNA sequence image from an autoradiograph. In part by interpolating a distribution of density peaks derived from the set of input data, the system is configured to generate a set of output data substantially identifying or presenting the bases in the DNA sequence in a linear fashion, corresponding to the linear order of nucleotides comprising the DNA sequence. The system thereby enables identification of substantially more bases than could be read by the human eye. Enhanced electrophoresis equipment and consumables may also be applied to generate a more predictable and linear set of input data and, in turn, to generate a set of output data identifying an even greater number of DNA bases in the sequence under analysis.
Type:
Grant
Filed:
December 9, 1994
Date of Patent:
September 9, 1997
Assignee:
Genomyx Corporation
Inventors:
Dean S. Burgi, Daniel M. Bartell, Donald D. Sleeter, Lucina P. Mastro
Abstract: A method for drying electrophoresis gel comprising, in combination, using a gaseous moisture removing medium, a gaseous moisture removing medium driving means, and an impingement means, whereby the gaseous moisture removing medium is driven by the gaseous moisture removing medium driving means across the impingement means to provide a flow of the gaseous moisture removing medium on the surface of the gel plates, whereby the flow induced by passage of the gaseous moisture removing medium through the impingement means thereby minimizes temperature gradients within the gel by forced convection and reduces drying time of the gel.