Abstract: Invertase is produced by a process wherein, first the yeast cells are disrupted to produce a disrupted cell suspension, second the disrupted cell suspension is adjusted to an acidic pH, third denatured undersired proteins are removed with the cell detritus and lastly the invertase is isolated. The improvement of the invention to the state of the art is that it comprises subjecting the disrupted cell suspension prior to removing the undesired proteins and cell detritus, to a pH of less than 4.5 and to a heat treatment in a continuous thermal denaturation system at a temperature in a range of from 44.degree. C. to about 51.degree. C.
Type:
Grant
Filed:
August 13, 1990
Date of Patent:
October 26, 1993
Assignee:
Gesellschaft fur Biotechnologische Forschung GmbH (GBF)
Inventors:
Helmut Hustedt, Kay Buntemeyer, Karl-Heinz Kroner, Bernhard Borner