Patents Assigned to Glykos Finland Oy
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Publication number: 20200040314Abstract: The present disclosure relates to compositions and methods useful for the production of heterologous proteins with reduced O-mannosylation in filamentous fungal cells, such as Trichoderma cells. More specifically, the invention provides a PMT-deficient filamentous fungal cell comprising a) at least a first mutation that reduces an endogenous protease activity compared to a parental filamentous fungal cell which does not have said first mutation, and, b) at least a second mutation in a PMT gene that reduces endogenous O-mannosyltransferase activity compared to a parental filamentous fungal cell which does not have said second mutation, wherein said filamentous fungal cell is selected from the group consisting of Trichoderma, Neurospora, Myceliophthora or Chrysosporium cell.Type: ApplicationFiled: September 7, 2018Publication date: February 6, 2020Applicant: Glykos Finland OYInventors: Jari Natunen, Jukka Hiltunen, Anne Huuskonen, Markku Saloheimo, Christian Ostermeier, Benjamin Patrick Sommer, Ramon Wahl
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Patent number: 10544440Abstract: The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.Type: GrantFiled: March 11, 2019Date of Patent: January 28, 2020Assignee: GLYKOS FINLAND OYInventors: Christopher Landowski, Anne Huuskonen, Ann Westerholm-Parvinen, Markku Saloheimo, Anne Kanerva, Jukka Hiltunen
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Patent number: 10513724Abstract: The present disclosure relates to compositions and methods useful for the production of recombinant glycoproteins in filamentous fungal cells, such as Trichoderma cells, wherein at least 90% (mol %), preferably at least 95% of the total neutral N-glycans of said produced recombinant glycoprotein are mammalian-like N-glycans. More specifically, the invention provides a filamentous fungal cell comprising i. one or more mutations that reduces or eliminates one or more endogenous protease activity compared to a parental filamentous fungal cell which does not have said mutation(s); ii. a polynucleotide encoding a heterologous catalytic subunit of oligosaccharyl transferase; iii. a recombinant polynucleotide for increasing ?1, 2 mannosidase activity;and, iv. a recombinant polynucleotide encoding said heterologous glycoprotein.Type: GrantFiled: July 21, 2015Date of Patent: December 24, 2019Assignee: GLYKOS FINLAND OYInventors: Jari Natunen, Anne Leppänen, Heidi Salminen, Jukka Hiltunen, Anne Kanerva, Annamari Heiskanen, Ann Westerholm-Parvinen, Georg Schmidt, Anne Huuskonen, Eero Mustalahti, Christopher Landowski, Markku Saloheimo, Juhani Saarinen, Benjamin Patrick Sommer, Ramon Wahl, Christian Ostermeier, Bernhard Helk
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Publication number: 20190309338Abstract: The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.Type: ApplicationFiled: March 11, 2019Publication date: October 10, 2019Applicants: Glykos Finland OY, Novartis AGInventors: Christopher Landowski, Anne Huuskonen, Ann Westerholm-Parvinen, Markku Saloheimo, Anne Kanerva, Jukka Hiltunen
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Patent number: 10435731Abstract: The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.Type: GrantFiled: July 10, 2014Date of Patent: October 8, 2019Assignee: Glykos Finland OyInventors: Christopher Landowski, Anne Huuskonen, Ann Westerholm-Parvinen, Markku Saloheimo, Anne Kanerva, Jukka Hiltunen
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Publication number: 20190276867Abstract: The present disclosure relates to compositions and methods useful for the production of heterologous proteins with increased N-glycosylation site occupancy in filamentous fungal cells, such as Trichoderma cells. More specifically, the invention provides a filamentous fungal cell comprising i. one or more mutation that reduces or eliminates one or more endogenous protease activity compared to a parental filamentous fungal cell which does not have said mutation(s), ii. a polynucleotide encoding a heterologous catalytic subunit of oligosaccharyl transferase, and iii. a polynucleotide encoding a heterologous glycoprotein, wherein said catalytic subunit of oligosaccharyl transferase is selected from Leishmania oligosaccharyl transferase catalytic subunits.Type: ApplicationFiled: March 26, 2019Publication date: September 12, 2019Applicant: Glykos Finland OyInventors: Jari Natunen, Christopher Landowski, Markku Saloheimo, Christian Ostermeier, Benjamin Patrick Sommer, Ramon Wahl
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Publication number: 20190233831Abstract: The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.Type: ApplicationFiled: January 18, 2019Publication date: August 1, 2019Applicant: GLYKOS FINLAND OYInventors: Christopher Landowski, Anne Huuskonen, Juhani Saarinen, Ann Westerholm-Parvinen, Anne Kanerva, Jari Natunen, Anna-Liisa Hänninen, Noora Salovuori, Merja PENTTILÄ, Markku Saloheimo
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Patent number: 10240159Abstract: The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.Type: GrantFiled: December 12, 2016Date of Patent: March 26, 2019Assignee: Glykos Finland OYInventors: Christopher Landowski, Anne Huuskonen, Juhani Saarinen, Ann Westerholm-Parvinen, Anne Kanerva, Jari Natunen, Anna-Liisa Hanninen, Noora Salovuori, Merja Penttila, Markku Saloheimo
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Publication number: 20180256732Abstract: The invention relates to a glycoprotein-toxic payload molecule conjugate, a toxic payload molecule-glycan conjugate, and a pharmaceutical composition. The invention further relates to a method for preparing the glycoprotein-toxic payload molecule conjugate, the method for modulating growth of a cell population and a method of treating tumour cells.Type: ApplicationFiled: September 14, 2017Publication date: September 13, 2018Applicant: GLYKOS FINLAND OYInventors: Tero SATOMAA, Jari HELIN, Filip S. EKHOLM
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Publication number: 20180228906Abstract: A molecule comprising a saccharide bound via an O-glycosidic bond to a hydroxyl group of a toxic payload molecule is disclosed. An antibody-drug conjugate comprising an antibody covalently bound to a toxic payload molecule, optionally via a linker group, and a saccharide bound via an O-glycosidic bond to a hydroxyl group of the toxic payload molecule is further disclosed.Type: ApplicationFiled: June 26, 2015Publication date: August 16, 2018Applicant: Glykos Finland OyInventors: Jari Helin, Juhani Saarinen, Tero Satomaa, Filip S. Ekholm
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Publication number: 20180215797Abstract: The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.Type: ApplicationFiled: August 11, 2016Publication date: August 2, 2018Applicant: Glykos Finland OYInventors: Christopher Landowski, Ann Westerholm-Parvinen, Markku Saloheimo
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Patent number: 10000734Abstract: The invention describes specific sialylated structures present on human stem cells and cell populations derived thereof. The invention is especially directed to methods to control the status of stem cells by observing changes in sialylation of the cells; and control of potential contaminations of biological materials; and reagents and methods used in connection with the cells in order to avoid alterations of the cell glycosylation by contaminating materials. The invention is further directed to novel stem cells, the glycosylation of which has been specifically altered.Type: GrantFiled: May 23, 2016Date of Patent: June 19, 2018Assignee: GLYKOS FINLAND OYInventors: Jarmo Laine, Taina Jaatinen, Heidi Anderson, Johanna Nystedt, Sari Tiitinen, Anita Laitinen, Ulla Impola, Tero Satomaa, Jari Natunen, Annamari Heiskanen, Maria Blomqvist, Anne Olonen
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Publication number: 20170275634Abstract: The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.Type: ApplicationFiled: December 12, 2016Publication date: September 28, 2017Applicant: Glykos Finland OYInventors: Christopher Landowski, Anne Huuskonen, Juhani Saarinen, Ann Westerholm-Parvinen, Anne Kanerva, Jari Natunen, Anna-Liisa Hanninen, Noora Salovuori, Merja Penttila, Markku Saloheimo
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Publication number: 20170189549Abstract: The present invention relates to a conjugate represented by the formula [D-L-Y—(CH2)n-O]m—P-T wherein T is a protein; P is a polymer selected from the group consisting of dextran, mannan, pullulan, hyaluronic acid, hydroxyethyl starch, chondroitin sulphate, heparin, heparin sulphate, polyalkylene glycol, Ficoll, polyvinyl alcohol, amylose, amylopectin, chitosan, cyclodextrin, pectin and carrageenan, or a derivative thereof; m is at least 1; n is in the range of 1 to 10; each Y is independently selected from the group consisting of S, NH and 1,2,3-triazolyl, wherein 1,2,3-triazolyl is optionally substituted; each L is independently absent or comprises a linker group covalently joining D and Y; and each D is a payload molecule.Type: ApplicationFiled: June 12, 2015Publication date: July 6, 2017Applicant: Glykos Finland OyInventors: Jari Helin, Juhani Saarinen, Filip S. Ekholm, Anne Leppanen
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Patent number: 9695454Abstract: Described herein are compositions including filamentous fungal cells, such as Trichoderma fungal cells, having reduced protease activity and expressing fucosylation pathway. Further described herein are methods for producing a glycoprotein having fucosylated N-glycan, using genetically modified filamentous fungal cells, for example, Trichoderma fungal cells, as the expression system.Type: GrantFiled: May 23, 2013Date of Patent: July 4, 2017Assignee: GLYKOS FINLAND OYInventors: Jari Natunen, Ann Westerholm-Parvinen, Hanna Salo, Jussi Joensuu, Heli Viskari, Christopher Landowski, Anne Huuskonen, Anne Kanerva, Anna-Liisa Hanninen, Noora Salovuori, Merja Penttila, Juhani Saarinen, Markku Saloheimo
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Publication number: 20170159094Abstract: The present disclosure relates to compositions and methods useful for the production of recombinant glycoproteins in filamentous fungal cells, such as Trichoderma cells, wherein at least 90% (mol %), preferably at least 95% of the total neutral N-glycans of said produced recombinant glycoprotein are mammalian-like N-glycans. More specifically, the invention provides a filamentous fungal cell comprising i. one or more mutations that reduces or eliminates one or more endogenous protease activity compared to a parental filamentous fungal cell which does not have said mutation(s); ii.a polynucleotide encoding a heterologous catalytic subunit of oligosaccharyl transferase; iii. a recombinant polynucleotide for increasing ?1, 2 mannosidase activity;and, iv. a recombinant polynucleotide encoding said heterologous glycoprotein.Type: ApplicationFiled: July 21, 2015Publication date: June 8, 2017Applicant: GLYKOS FINLAND OYInventors: Jari Natunen, Anne Leppänen, Heidi Salminen, Jukka Hiltunen, Anne Kanerva, Annamari Heiskanen, Ann Westerholm-Parvinen, Georg Schmidt, Anne Huuskonen, Eero Mustalahti, Christopher Landowski, Markku Saloheimo, Juhani Saarinen, Benjamin Patrick Sommer, Ramon Wahl, Christian Ostermeier, Bernhard Helk
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Patent number: 9567596Abstract: The present disclosure relates to compositions and methods useful for the production of heterologous proteins in filamentous fungal cells.Type: GrantFiled: January 4, 2013Date of Patent: February 14, 2017Assignee: Glykos Finland OYInventors: Christopher Landowski, Anne Huuskonen, Juhani Saarinen, Ann Westerholm-Parvinen, Anne Kanerva, Jari Natunen, Anna-Liisa Hanninen, Noora Salovuori, Merja Penttila, Markku Saloheimo
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Publication number: 20170002337Abstract: The present disclosure relates to recombinant proteins having N-acetylglucosaminyltransferase activity. The present disclosure further relates to methods for producing complex N-glycans including the steps of providing host cells containing such recombinant proteins and culturing the host cells such that the recombinant proteins are expressed.Type: ApplicationFiled: July 8, 2016Publication date: January 5, 2017Applicant: Glykos Finland OyInventors: Jari Natunen, Anne Kanerva, Jukka Hiltunen, Markku Saloheimo, Heli Viskari, Anne Huuskonen
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Publication number: 20170002095Abstract: The present invention describes glycans, which are specifically expressed by certain cancer cells, tumours and other malignant tissues. The present invention describes methods to detect cancer specific glycans as well as methods for the production of reagents binding to said glycans. The invention is also directed to the use of said glycans and reagents binding to them for the diagnostics of cancer and malignancies. Furthermore, the invention is directed to the use of said glycans and reagents binding to them for the treatment of cancer and malignancies. Moreover, the present invention comprises efficient methods to differentiate between malignant and benign tumors by analyzing glycan structures.Type: ApplicationFiled: September 14, 2016Publication date: January 5, 2017Applicant: Glykos Finland OyInventors: Tero SATOMAA, Jari NATUNEN, Annamari HEISKANEN, Anne OLONEN, Juhani SAARINEN, Noora SALOVUORI, Jari HELIN
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Publication number: 20160333309Abstract: The invention describes specific sialylated structures present on human stem cells and cell populations derived thereof. The invention is especially directed to methods to control the status of stem cells by observing changes in sialylation of the cells; and control of potential contaminations of biological materials; and reagents and methods used in connection with the cells in order to avoid alterations of the cell glycosylation by contaminating materials. The invention is further directed to novel stem cells, the glycosylation of which has been specifically altered.Type: ApplicationFiled: May 23, 2016Publication date: November 17, 2016Applicant: Glykos Finland OyInventors: Jarmo LAINE, Taina JAATINEN, Heidi ANDERSON, Johanna NYSTEDT, Sari TIITINEN, Anita LAITINEN, Ulla IMPOLA, Tero Satomaa, Jari NATUNEN, Annamari Heiskanen, Maria Blomqvist, Anne Olonen