Abstract: A method for preparing a biopesticide preparation for preventing and controlling Solenopsis invicta comprising: a: preparing an agent containing pathogenic microorganisms for preventing and controlling Solenopsis invicia, the agent comprises Beauveria bassiana and sodium chloride, with a Beauveria bassiana spores to sodium chloride ratio of (1-8)×20 billion spores: 4.0-7.5 g; b: placing the agent into a solid container, and adding a corresponding amount of solution in a liquid container; inserting the solid container into the liquid container, wherein a retaining ring is stuck at the opening of the liquid container, a rubber gasket is pressed against the retaining ring, and a lid covers the rubber gasket and the retaining ring and is fixed to the opening of the liquid container.

Abstract: An artificial diet for ghost moth larvae and a preparation method thereof. The artificial diet comprises root of Polygonum viviparum, soybean powder, bran, yeast powder, sucrose, casein, agar, water, choline chloride, ascorbic acid, sorbic acid, nipagin and multivitamin. After being fed for 60 days, third-instar larvae fed with the artificial diet showed an average survival rate of up to 92.67%, higher than those fed with carrots with an average survival rate of 90.33%. After being fed for 140 days, the larvae fed with the artificial diet showed an average survival rate of up to 86.67%, significantly higher than those fed with carrots with an average survival rate of 73.67%. Also, the larvae fed with the artificial diet showed significant increase in body weight, without the contamination of bacterium and fungi.

Abstract: The use of Pyemotes zhonghuajia for preparing biological control agents of Diaphorina citri. The use of Pyemotes zhonghuajia for preparing agents for controlling Diaphorina citri. By employing Pyemotes zhonghuajia to control Diaphorina citri, it is a biological control method which can avoid the problems caused by chemical pesticides such as environmental pollution, health risk and increased pest resistance to the pesticides.

Abstract: An artificial feeding method at low altitude for a host insect ghost moth of Ophiocordyceps sinensis including selecting an appropriate disinfectant for performing surface disinfection on ghost moth eggs, and then placing the disinfected eggs in sterile humus; feeding ghost moth larvae with carrots, disinfecting and detecting the fed carrots, and culturing the larvae till male and female pupae are obtained; identifying male and female pupae of the ghost moths, placing them at different culture temperatures, wherein the culture temperature for the male pupae is 2-6° C. lower than that of the female pupae, resulting synchronously emerged male and female adults; placing the emerged male and female adults in an adult cage with simulated plants for mating and staying of the adults, maintaining a humidity over 80%, and employing 50-100 Lux shimmer for facilitating activity and mating of the adults.

Abstract: A method for artificial cultivation of Ophiocordyceps sinensis fruiting bodies. The method comprises: inoculating Ophiocordyceps sinensis into a sterile rice medium, cultivating at 9-13° C. for 40-60 days, after the medium is covered with mycelia, performing low-temperature induction at 1-8° C. for 60-80 days to develop a fruiting body primordium, and transferring the cultivation to 11-16° C. till harvest of the fruiting bodies. The method requires no low-oxygen environment, which can reduce cultivation cost; it only needs 3-4 months from induction to harvest of fruiting bodies: the rice medium for use has a low cost, which is suitable for commercial cultivation of Ophiocordyceps sinensis fruiting bodies.

Abstract: The use of Pyemotes zhonghuajia for preparing biological control agents of Diaphorina citri. The use of Pyemotes zhonghuajia for preparing agents for controlling Diaphorina citri. By employing Pyemotes zhonghuajia to control Diaphorina citri, it is a biological control method which can avoid the problems caused by chemical pesticides such as environmental pollution, health risk and increased pest resistance to the pesticides.

Abstract: An artificial diet for ghost moth larvae and a preparation method thereof. The artificial diet comprises root of Polygonum viviparum, soybean powder, bran, yeast powder, sucrose, casein, agar, water, choline chloride, ascorbic acid, sorbic acid, nipagin and multivitamin. After being fed for 60 days, third-instar larvae fed with the artificial diet showed an average survival rate of up to 92.67%, higher than those fed with carrots with an average survival rate of 90.33%. After being fed for 140 days, the larvae fed with the artificial diet showed an average survival rate of up to 86.67%, significantly higher than those fed with carrots with an average survival rate of 73.67%. Also, the larvae fed with the artificial diet showed significant increase in body weight, without the contamination of bacterium and fungi.

Abstract: The present invention discloses a rapid diagnosis method of citrus huanglongbing. In the present invention, the accuracy of diagnosis of citrus huanglongbing is significantly improved in the following manners: eliminating the interference of residual starch by effectively removing the residual starch contained in the leaf; eliminating the interference of chlorophyll by effectively removing chlorophyll; and directly developing the color on the leaf rather than mixing and grinding the leaf in water, so as to avoid the problem that the accuracy of color development reaction conducted by mixing the leaf with water is liable to be interfered since the starch is insoluble in water. Therefore a rapid diagnosis of the citrus huanglongbing with much higher accuracy is achieved, which provides effective detection means for the control of the citrus huanglongbing and is beneficial for the control of the citrus huanglongbing.