Abstract: The present invention provides methods and apparatus for detecting and discriminating multiple analytes within a test sample which are rapid, accurate, and convenient enough for routine use in a clinical laboratory. In particular, the present invention provides methods and apparatus for permitting multiple PCR-amplified target nucleic acid sequence hybrids within a single sample, labeled with different fluorescent dyes, to be spectrally distinguished using the read-out data directly from a fluorescence reader instrument.
Type:
Grant
Filed:
September 8, 1997
Date of Patent:
January 19, 1999
Assignee:
Gull Laboratories
Inventors:
Michael J. Glass, Jana Coombs, Sharon L. Malmstrom, Linxian Wu
Abstract: The present invention provides methods and apparatus for detecting and discriminating multiple analytes within a test sample which are simple, user-friendly, cost-effective and fast. In particular, it is preferred that the overall time for sample preparation, nucleic acid sequence amplification, and nucleic acid sequence differentiation be about 5 hours or less.
Type:
Grant
Filed:
August 6, 1996
Date of Patent:
December 8, 1998
Assignee:
Gull Laboratories
Inventors:
Linxian Wu, Jana Coombs, Sharon L. Malmstrom, Michael J. Glass
Abstract: The present invention provides methods and apparatus for detecting and discriminating multiple analytes within a test sample which are simple, user-friendly, cost-effective and fast. In particular, it is preferred that the overall time for sample preparation, nucleic acid sequence amplification, and nucleic acid sequence differentiation be about 5 hours or less.
Type:
Grant
Filed:
August 6, 1996
Date of Patent:
May 26, 1998
Assignee:
Gull Laboratories, Inc.
Inventors:
Linxian Wu, Jana Coombs, Sharon L. Malmstrom, Michael J. Glass
Abstract: The present invention provides methods and apparatus for detecting and discriminating multiple analytes within a test sample which are simple, user-friendly, cost-effective and fast. In particular, it is preferred that the overall time for sample preparation, nucleic acid sequence amplification, and nucleic acid sequence differentiation be about 5 hours or less.
Type:
Grant
Filed:
August 7, 1996
Date of Patent:
May 19, 1998
Assignee:
Gull Laboratories, Inc.
Inventors:
Linxian Wu, Jana Coombs, Sharon L. Malmstrom, Michael J. Glass
Abstract: The present invention provides methods and apparatus for detecting and discriminating multiple analytes within a test sample which are simple, user-friendly, cost-effective and fast. In particular, it is preferred that the overall time for sample preparation, nucleic acid sequence amplification, and nucleic acid sequence differentiation be about 5 hours or less.
Type:
Grant
Filed:
August 7, 1996
Date of Patent:
April 14, 1998
Assignee:
Gull Laboratories, Inc.
Inventors:
Linxian Wu, Jana Coombs, Sharon L. Malmstrom, Michael J. Glass
Abstract: The present invention provides methods and apparatus for detecting and discriminating multiple analytes within a test sample which are rapid, accurate, and convenient enough for routine use in a clinical laboratory. In particular, the present invention provides methods and apparatus for permitting multiple PCR-amplified target nucleic acid sequence hybrids within a single sample, labeled with different fluorescent dyes, to be spectrally distinguished using the read-out data directly from a fluorescence reader instrument.
Type:
Grant
Filed:
March 5, 1996
Date of Patent:
March 3, 1998
Assignee:
Gull Laboratories
Inventors:
Michael J. Glass, Jana Coombs, Sharon L. Malmstrom, Linxian Wu
Abstract: The present invention provides methods and apparatus for detecting and discriminating multiple analytes within a test sample which are simple, user-friendly, cost-effective and fast. In particular, it is preferred that the overall time for sample preparation, nucleic acid sequence amplification, and nucleic acid sequence differentiation be about 5 hours or less.
Type:
Grant
Filed:
January 16, 1996
Date of Patent:
March 18, 1997
Assignee:
Gull Laboratories
Inventors:
Linxian Wu, Jana Coombs, Sharon L. Malmstrom, Michael J. Glass
Abstract: A multiple stage anti-microbial apparatus and methods are disclosed, which provide capacity to process a handpiece between patients including soaking, de-overburdening via use of ultrasonic energy, treating with exposing to anti-microbial heat, accommodating coordinated though separate autoclaving, and accommodating contiguously packaging in a heat shrunk sheath after multiple anti-microbial treatment to prevent later contamination through the air. The distal portion of the heat shrunk sheath is initially removed along weakened paths to expose the operative head of the handpiece, accommodating grasping of the handpiece handle through the remainder of the sheath during use of the handpiece on the patient. After such use, the remainder of the sheath is removed along a weakened path and discarded.The anti-microbial apparatus comprises a portable casing with one or more exterior lids or covers. An ultrasonic basin provides a bath for soaking, liquid anti-microbial activity and removal of debris.
Abstract: A multiple stage anti-microbial apparatus and methods are disclosed, which provide capacity to process a handpiece between patients including soaking, de-overburdening via use of ultrasonic energy, treating with exposing to anti-microbial heat, accommodating coordinated though separate autoclaving, and accommodating contiguously packaging in a heat shrunk sheath after multiple anti-microbial treatment to prevent later contamination through the air. The distal portion of the heat shrunk sheath is initially removed along weakened paths to expose the operative head of the handpiece, accommodating grasping of the handpiece handle through the remainder of the sheath during use of the handpiece on the patient. After such use, the remainder of the sheath is removed along a weakened path and discarded.The anti-microbial apparatus comprises a portable casing with one or more exterior lids or covers. An ultrasonic basin provides a bath for soaking, liquid anti-microbial activity and removal of debris.